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Title:Določanje sledov benzo(a)pirena v vodi s plinsko kromatografijo z masno spektrometrijo : magistrsko delo
Authors:ID Štrakl, Anja (Author)
ID Islamčević Razboršek, Maša (Mentor) More about this mentor... New window
Files:.pdf MAG_Strakl_Anja_2024.pdf (4,16 MB)
MD5: 6B66A629767C216E7627B8563DE429AC
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Policiklični aromatski ogljikovodiki (PAO) so močno lipofilne organske komponente, ki so vseprisotne v okolju in v zadnjih letih vzbujajo veliko pozornost zaradi svoje rakotvornosti. Nastajajo med nepopolnim izgorevanjem organskih snovi, tudi med pripravo živil in pri kajenju. Vedno jih najdemo kot mešanico posameznih komponent. V vodi so, zaradi nizke vodotopnosti in velike afinitete do trdnih delcev, njihove koncentracije zelo nizke. Prisotnost PAO v vodah tako kaže na onesnaženje. Namen magistrske naloge je bil kvantitativno določiti benzo(a)piren v vzorcih površinskih vod na zelo nizkih koncentracijskih nivojih. Uredba o stanju površinskih voda določa okoljski standard kakovosti za benzo(a)piren, ki znaša 0,05 ng/L, kar zahteva uporabo občutljivih instrumentalnih analiznih tehnik. Vsebnost benzo(a)pirena v vodi smo določili z uporabo optimizirane analizne metode, ki temelji na že uporabljeni in validirani metodi. Uporabili smo ekstrakcijo tekoče-tekoče z diklorometanom v lijih ločnikih. Po ekstrakciji smo ločeni organski fazi dodali brezvodni natrijev sulfat in ustrezne raztopine standardov ter jo skoncentrirali na željeni volumen. V tako pripravljenih vzorcih smo benzo(a)piren določili s tehniko plinske kromatografije z masno selektivnim detektorjem (GC/MSD). Kromatograme smo posneli z metodo izbranih ionov (SIM – selected ion monitoring). Za kvantitativno določitev smo uporabili mešano raztopino šestnajstnih internih standardov. Rezultati so pokazali, da je optimizirana analizna metoda primerna za zanesljivo določanje sledov benzo(a)pirena v vodi, saj so bili izkoristki pri določanju ponovljivi in znotraj predpisanega kriterija. Ugotovili smo prisotnost vpliva matrice oz. osnove vzorca, saj so bili izkoristki v topilu previsoki. Zato smo pripravili umeritveno krivuljo v matrici vode, kjer smo zadostili kriterijem (izkoristki so bili od 70 % do 110 %).
Keywords:policiklični aromatski ogljikovodiki, benzo(a)piren, plinska kromatografija, masni spektrometer, ekstrakcija tekoče-tekoče
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[A. Štrakl]
Year of publishing:2024
Number of pages:1 spletni vir (1 datoteka PDF (IX, 71 f.))
PID:20.500.12556/DKUM-90535 New window
UDC:543.5:[543.38:547.6](043.2)
COBISS.SI-ID:221660419 New window
Publication date in DKUM:26.09.2024
Views:0
Downloads:13
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
:
ŠTRAKL, Anja, 2024, Določanje sledov benzo(a)pirena v vodi s plinsko kromatografijo z masno spektrometrijo : magistrsko delo [online]. Master’s thesis. Maribor : A. Štrakl. [Accessed 26 March 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=90535
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:07.09.2024

Secondary language

Language:English
Title:Trace level determination of benzo(a)pyrene in water by gas chromatography with mass spectrometry
Abstract:Polycyclic aromatic hydrocarbons (PAHs) are highly lipophilic organic compounds that are ubiquitous in the environment and have attracted much attention in recent years due to their carcinogenicity. They are formed during the incomplete combustion of organic substances, including during food preparation and smoking. They always occur as a mixture of individual compounds. Their concentrations in water are very low due to their low solubility in water and their high affinity for solid particles. The presence of PAHs in water therefore indicates pollution. The aim of the master's thesis was the quantitative determination of benzo(a)pyrene in surface water samples at very low concentration levels. Ordinance on the Status of Surface Waters sets an environmental quality standard for benzo(a)pyrene of 0,05 ng/L, which requires the use of sensitive instrumental analysis techniques. The level of benzo(a)pyrene in water was determined using an optimized analytical method based on a previously used and validated method. We used a liquid-liquid extraction with dichloromethane in separatory funnels. After extraction, anhydrous sodium sulfate and appropriate standard solutions were added to the separated organic phase and concentrated to the desired volume. Benzo(a)pyrene was determined in the samples prepared in this way using the gas chromatography technique with mass selective detector (GC/MSD). The chromatograms were recorded using the selected ion monitoring (SIM) method. A mixed solution of sixteen internal standards was used for the quantitative determination. The results showed that the optimized analytical method is suitable for the reliable determination of traces of benzo(a)pyrene in water, as the determination results were reproducible and within the specified criteria. We found an influence of the matrix, as the yields in the solvent were too high. Therefore, we prepared a calibration curve in the water matrix in which we met the criteria (the yields were between 70 % and 110 %).
Keywords:polycyclic aromatic hydrocarbons, benzo(a)pyrene, gas chromatography, mass spectrometer, liquid-liquid extraction


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