| Title: | Bioactive functional nanolayers of chitosan-lysine surfactant with single- and mixed-protein-repellent and antibiofilm properties for medical implants |
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| Authors: | ID Ajdnik, Urban (Author)
ID Fras Zemljič, Lidija (Author)
ID Plohl, Olivija (Author)
ID Pérez, Lourdes (Author)
ID Trček, Janja (Author)
ID Bračič, Matej (Author)
ID Mohan, Tamilselvan (Author) |
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| Files: |  ajdnik-et-al-2021-bioactive-functional-nanolayers-of-chitosan-lysine-surfactant-with-single-and-mixed-protein-repellent.pdf (4,24 MB)
MD5: AB33C47B2993FC98239EDF17508702AC
 https://pubs.acs.org/doi/10.1021/acsami.1c01993#
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| Language: | English |
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| Work type: | Unknown |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FS - Faculty of Mechanical Engineering
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| Abstract: | Medical implant-associated infections resulting from biofilm formation triggered by unspecific protein adsorption arethe prevailing cause of implant failure. However, implant surfaces rendered with multifunctional bioactive nanocoatings offer apromising alternative to prevent the initial attachment of bacteria and effectively interrupt biofilm formation. The need to researchand develop novel and stable bioactive nanocoatings for medical implants and a comprehensive understanding of their properties incontact with the complex biological environment are crucial. In this study, we developed an aqueous stable and crosslinker-freepolyelectrolyte−surfactant complex (PESC) composed of a renewable cationic polysaccharide, chitosan, a lysine-based anionicsurfactant (77KS), and an amphoteric antibiotic, amoxicillin, which is widely used to treat a number of infections caused by bacteria.We successfully introduced the PESC as bioactive functional nanolayers on the“model”and“real”polydimethylsiloxane (PDMS)surfaces under dynamic and ambient conditions. Besides their high stability and improved wettability, these uniformly depositednanolayers (thickness: 44−61 nm) with mixed charges exhibited strong repulsion toward three model blood proteins (serumalbumin,fibrinogen, andγ-globulin) and their competitive interactions in the mixture in real-time, as demonstrated using a quartzcrystal microbalance with dissipation (QCM-D). The functional nanolayers with a maximum negative zeta potential (ζ:−19 to−30mV at pH 7.4), water content (1628−1810 ng cm−2), and hydration (low viscosity and elastic shear modulus) correlated with themass, conformation, and interaction nature of proteins. In vitro antimicrobial activity testing under dynamic conditions showed thatthe charged nanolayers actively inhibited the growth of both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcusaureus) bacteria compared to unmodified PDMS. Given the ease of fabrication of multifunctional and charged biobased coatingswith simultaneous protein-repellent and antimicrobial activities, the limitations of individual approaches could be overcome leadingto a better and advanced design of various medical devices (e.g., catheters, prosthetics, and stents). |
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| Keywords: | silicone implants, protein-repellent, antimicrobial, chitosan, lysine, bioactive coatings, adsorption, QCM-D |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 29.01.2021 |
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| Article acceptance date: | 04.05.2021 |
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| Publication date: | 17.05.2021 |
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| Publisher: | ACS Publications |
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| Year of publishing: | 2021 |
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| Number of pages: | str. 23352–23368 |
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| Numbering: | Vol. 13, iss. 20 |
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| PID: | 20.500.12556/DKUM-88322  |
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| UDC: | 677.017:579 |
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| ISSN on article: | 1944-8244 |
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| COBISS.SI-ID: | 63734787 |
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| DOI: | 10.1021/acsami.1c01993 |
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| Publication date in DKUM: | 15.04.2024 |
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| Views: | 266 |
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| Downloads: | 17 |
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| Metadata: |    |
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| Categories: | Misc.
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AJDNIK, Urban, FRAS ZEMLJIČ, Lidija, PLOHL, Olivija, PÉREZ, Lourdes, TRČEK, Janja, BRAČIČ, Matej and MOHAN, Tamilselvan, 2021, Bioactive functional nanolayers of chitosan-lysine surfactant with single- and mixed-protein-repellent and antibiofilm properties for medical implants. ACS applied materials & interfaces [online]. 2021. Vol. 13, no. 20. [Accessed 23 April 2025]. DOI 10.1021/acsami.1c01993. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=88322
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