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Title:The role of ascorbate–glutathione system and volatiles emitted by insect-damaged lettuce roots as navigation signals for insect and slug parasitic nematodes
Authors:ID Laznik, Žiga (Author)
ID Križman, Mitja (Author)
ID Zekič, Jure (Author)
ID Roškarič, Mihaela (Author)
ID Trdan, Stanislav (Author)
ID Urbanek Krajnc, Andreja (Author)
Files:.pdf Laznik-2023-The_Role_of_Ascorbate-Glutathione.pdf (3,96 MB)
MD5: B7CFC9C066180014A0C869FBFEC95870
 
URL https://doi.org/10.3390/insects14060559
 
Language:English
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:FKBV - Faculty of Agriculture and Life Sciences
Abstract:The effect of wireworm-damaged lettuce roots on the antioxidative defense system (ascorbate–glutathione cycle, photosynthetic pigments) and movement of insect/slug parasitic nematodes towards determined root exudates was studied in a glasshouse experiment. Lettuce seedlings were grown in a substrate soil in the absence/presence of wireworms (Elateridae). The ascorbate– glutathione system and photosynthetic pigments were analyzed by HPLC, while volatile organic compounds (VOC) emitted by lettuce roots were investigated by GC-MS. Herbivore-induced root compounds, namely 2,4-nonadienal, glutathione, and ascorbic acid, were selected for a chemotaxis assay with nematodes Steinernema feltiae, S. carpocapsae, Heterorhabditis bacteriophora, Phasmarhabditis papillosa, and Oscheius myriophilus. Root pests had a negative effect on the content of photosynthetic pigments in the leaves of infested plants, indicating that they reacted to the presence of reactive oxygen species (ROS). Using lettuce as a model plant, we recognized the ascorbate–glutathione system as a redox hub in defense response against wireworms and analyzed its role in root-exudate-mediated chemotaxis of nematodes. Infected plants also demonstrated increased levels of volatile 2,4-nonadienal. Entomopathogenic nematodes (EPNs, S. feltiae, S. carpocapsae, and H. bacteriophora) proved to be more mobile than parasitic nematodes O. myriophilus and P. papillosa towards chemotaxis compounds. Among them, 2,4-nonadienal repelled all tested nematodes. Most exudates that are involved in belowground tritrophic interactions remain unknown, but an increasing effort is being made in this field of research. Understanding more of these complex interactions would not only allow a better understanding of the rhizosphere but could also offer ecologically sound alternatives in the pest management of agricultural systems.
Keywords:lettuce, wireworms, ascorbate–glutathione system, root volatile organic compounds, entomopathogenic nematodes, slug parasitic nematodes
Publication status:Published
Publication version:Version of Record
Submitted for review:21.04.2023
Article acceptance date:14.06.2023
Publication date:15.06.2023
Publisher:MDPI
Year of publishing:2023
Number of pages:Str. 1-18
Numbering:Letn. 14, Št. 6, št. članka 559
PID:20.500.12556/DKUM-87074 New window
UDC:632
ISSN on article:2075-4450
COBISS.SI-ID:156166915 New window
DOI:10.3390/insects14060559 New window
Publication date in DKUM:15.02.2024
Views:395
Downloads:20
Metadata:XML DC-XML DC-RDF
Categories:Misc.
:
LAZNIK, Žiga, KRIŽMAN, Mitja, ZEKIČ, Jure, ROŠKARIČ, Mihaela, TRDAN, Stanislav and URBANEK KRAJNC, Andreja, 2023, The role of ascorbate–glutathione system and volatiles emitted by insect-damaged lettuce roots as navigation signals for insect and slug parasitic nematodes. Insects [online]. 2023. Vol. 14, no. Št. 6,  članka 559, p. 1–18. [Accessed 23 January 2025]. DOI 10.3390/insects14060559. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=87074
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Record is a part of a journal

Title:Insects
Shortened title:Insects
Publisher:MDPI AG
ISSN:2075-4450
COBISS.SI-ID:519122457 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:J4-3090-2021
Name:Pomen hlapnih izločkov korenin gojenih rastlin pri posredni obrambi pred talnimi škodljivimi žuželkami

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P4-0431-2022
Name:Kmetijstvo naslednje generacije

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0005-2020
Name:Funkcionalna živila in prehranska dopolnila

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0164-2019
Name:Raziskave za zagotavljanje varne hrane in zdravja

Funder:Other - Other funder or multiple funders
Funding programme:Ministrstvo za kmetijstvo, gozdarstvo in prehrano
Project number:-
Name:Expert Tasks from the Field of Plant Protection

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:15.06.2023

Secondary language

Language:Slovenian
Keywords:solata, strune, škodljivci rastlin, hlapne organske spojine, entomopatogene ogorčice, parazitske ogorčice


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