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Title:Vpliv procesnih parametrov na kakovost in obstojnost ekstrakta ameriškega slamnika (Echinacea purpurea) : diplomsko delo
Authors:ID Fingušt, Katja (Author)
ID Knez Marevci, Maša (Mentor) More about this mentor... New window
ID Cör Andrejč, Darija (Comentor)
Files:.pdf UN_Fingust_Katja_2023.pdf (2,30 MB, This file will be accessible after 08.09.2026)
MD5: 0A0F66C84446BAF235963414CC711870
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:V diplomskem delu smo raziskovali vpliv procesnih parametrov na kakovost in obstojnost ekstrakta ameriškega slamnika. Želeli smo pridobiti stabilne ekstrakte ter izolirati čim višje vsebnosti polifenolov in polisaharidov. Najprej smo izvedli sejalno analizo, s katero smo določili mediansko zrno, najpogosteje zastopano zrno in koeficient zrnatosti, ter ugotovili, da je bila zrnatost neenakomerna. V laboratoriju smo izvajali vročo in hladno maceracijo ter ultrazvočno ekstrakcijo. Uporabljali smo pet različnih topil: destilirano vodo, absolutni etanol ter mešanice etanol:voda (70:30), etanol:voda (50:50) ter etil acetat:voda (70:30). Ko smo izvedli vse ekstrakcije, smo pričeli z analiznim delom, kjer smo s spektrofotometričnimi metodami določili vsebnosti totalnih fenolov in proantocianidinov ter antioksidativno aktivnost ekstraktov in ugotovili, da je imel ekstrakt, pridobljen z vročo maceracijo in topilom etanol:voda (70:30), najvišji izkoristek, najvišjo vsebnost totalnih fenolov in najvišjo antioksidativno aktivnost. Najnižji izkoristek pa je imel ekstrakt, ki smo ga pridobili z ultrazvočno ekstrakcijo, kjer smo kot topilo uporabili absolutni etanol. Prav tako je imel ta ekstrakt tudi najnižjo vsebnost totalnih fenolov in proantocianidinov. Večinoma so ekstrakti pokazali visoke vsebnosti fenolnih spojin. Etanolni ekstrakti so pokazali višje vsebnosti fenolnih spojin, prisotnost vode pa je povečala ekstrakcijo proantocianidinov, kar je potrdilo naši hipotezi. Kot predvidevano smo najvišje izkoristke dobili pri vroči maceraciji. Kasneje smo izvedli mikroenkapsulacijo in pridobljenim formulatom določili antioksidativno delovanje ter ugotovili, da je bila učinkovitost precej nizka.
Keywords:ameriški slamnik, ekstrakcija, totalni fenoli, antioksidanti, proantocianidini, sejalna analiza
Place of publishing:Maribor
Place of performance:Maribor
Publisher:K. Fingušt
Year of publishing:2023
Number of pages:1 spletni vir (1 datoteka PDF (X, 36 f.))
PID:20.500.12556/DKUM-85247 New window
UDC:66.061.3:582.991.1(043.2)
COBISS.SI-ID:167501571 New window
Publication date in DKUM:14.09.2023
Views:573
Downloads:0
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
:
FINGUŠT, Katja, 2023, Vpliv procesnih parametrov na kakovost in obstojnost ekstrakta ameriškega slamnika (Echinacea purpurea) : diplomsko delo [online]. Bachelor’s thesis. Maribor : K. Fingušt. [Accessed 25 March 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=85247
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:23.08.2023

Secondary language

Language:English
Title:Impact of process parameters on the quality and stability of Echinacea purpurea extract
Abstract:In the thesis the influence of process parameters on the quality and stability of purple coneflower (Echinacea purpurea) were investigated. The aim was to obtain stable extracts and isolate the highest possible polyphenols and polysaccharides contents. First, a sieve analysis was carried out to determine the median grain, the most commonly represented grain and the coefficient of grain size, and it was found that the grain size was uneven. Hot and cold maceration and ultrasonic extraction were performed. Five different solvents were used: distilled water, absolute ethanol and ethanol:water (70:30), ethanol:water (50:50) and ethyl acetate:water (70:30) mixtures. After the extractions, the analytical part was followed by the determination of the total phenolic and proanthocyanidin contents and antioxidant activity of the extracts by spectrophotometric methods. The extract obtained by hot maceration with ethanol:water (70:30) solvent had the highest yield, the highest total phenolic content and antioxidant activity. However, the extract obtained by ultrasonic extraction using absolute ethanol as solvent had the lowest yield. This extract had the lowest total phenols and proanthocyanidins content. Most of the extracts showed high contents of phenolic compounds. Ethanol extracts resulted higher phenolic compounds and the presence of water increased the extraction of proanthocyanidins, which confirmed our hypothesis. As expected, the highest yields were obtained with hot maceration. Subsequently, microencapsulation was conducted and the antioxidant activity of the resulting formulations was determined and the value was found to be quite low.
Keywords:purple coneflower, extraction, total phenols, antioxidants, proanthocyanidins, sieve analysis


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