| | SLO | ENG | Cookies and privacy

Bigger font | Smaller font
First page > Show document

Show document Help

Title:Bioaktivne snovi iz olupkov manga za kozmetično industrijo : diplomsko delo univerzitetnega študijskega programa I. stopnje
Authors:ID Dolgov, Melani (Author)
ID Leitgeb, Maja (Mentor) More about this mentor... New window
ID Primožič, Mateja (Comentor)
Files:.pdf UN_Dolgov_Melani_2022.pdf (5,39 MB)
MD5: D05623AEFD1B24B01C19A486767FC3EC
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Mango velja za najbolj pogosto gojeno tropsko sadje po vsem svetu. Poleg svežega sadja ga lahko predelamo v sokove, nektarje, koncentrate in marmelade, vendar med samo predelavo ostane veliko odpadnega materiala, kot so olupki, ki predstavljajo resen okoljski problem. Ravno olupki predstavljajo bogat vir polifenolnih spojin, flavonoidov, vitamina C in zaradi tega imajo protimikrobne in antioksidativne lastnosti. Za izvedbo eksperimentalnega dela diplomske naloge smo pripravili 4 vzorce, in sicer vodni ekstrakt suhih mangovih olupkov, etanolni ekstrakt suhih mangovih olupkov, vodni ekstrakt svežih mangovih olupkov in etanolni ekstrakt svežih mangovih olupkov ter jih analizirali s pomočjo spektrofotometričnih analiz. Protimikrobno delovanje izolatov smo ocenili s Kirby – Bauerjevo in mikrodilucijsko metodo. S Kirby – Bauerjevo metodo smo z merjenjem cone inhibicije določevali inhibitorne lastnosti izolatov na rast različnih mikroorganizmov, in sicer Gram-negativnih bakterij (Escherichia coli in Pseudomonas aeruginosa), Gram-pozitivnih bakterij (Staphylococcus aureus in Bacillus cereus) ter gliv (Candida albicans in Aspergillus brasiliensis). Z mikrodilucijsko metodo pa samo določili protimikrobno delovanje izolatov na rast bakterij, saj smo predhodno s Kirby – Bauerjevo metodo dosegli uspešno inhibicijo le na rasti bakterij. S pomočjo mikrodilucijske metode smo določili minimalno inhibitorno koncentracijo izolatov. Spektrofotometrične analize vzorcev so potrdile višjo koncentracijo totalnih fenolov in proantocianidinov ter višjo antioksidativno aktivnost etanolnih ekstraktov v primerjavi z vodnimi ekstrakti. Iz izmerjenih con inhibicije smo s Kirby – Bauerjevo metodo dokazali protimikrobno delovanje na rast Gram-pozitivnih in Gram-negativnih bakterij, medtem ko testirani vzorci niso zavirali rast gliv. Rezultati mikrodilucijske metode so pokazali, da so bile boljše protimikrobne lastnosti izražene proti Gram-pozitivnim bakterijam v primerjavi z Gram-negativnimi. Glede na dobljene rezultate smo dokazali, da so olupki manga bogat vir bioaktivnih snovi s protimikrobnim in antioksidativnim delovanjem, zato bi se lahko v prihodnosti izolati iz olupkov manga uporabljali v različnih industrijskih panogah, kot so kozmetična, prehrambna in farmacevtska. Z njihovo nadaljnjo uporabo bi lahko zmanjšali količino odpadnega materiala, ki nastane med predelavo manga in s tem zmanjšali vpliv odpadkov na okolje.
Keywords:olupki manga, mikroorganizmi, bioaktivne snovi, protimikrobnost, minimalna inhibitorna koncentracija
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[M. Dolgov]
Year of publishing:2022
Number of pages:1 spletni vir (1 datoteka PDF (XI, 70 f.))
PID:20.500.12556/DKUM-82295 New window
UDC:[604.4:615.33]:634.441(043.2)
COBISS.SI-ID:130711555 New window
Publication date in DKUM:19.09.2022
Views:723
Downloads:106
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
:
DOLGOV, Melani, 2022, Bioaktivne snovi iz olupkov manga za kozmetično industrijo : diplomsko delo univerzitetnega študijskega programa I. stopnje [online]. Bachelor’s thesis. Maribor : M. Dolgov. [Accessed 29 April 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=82295
Copy citation
  
Average score:
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
(0 votes)
Your score:Voting is allowed only for logged in users.
Share:Bookmark and Share


Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Licences

License:CC BY-ND 4.0, Creative Commons Attribution-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nd/4.0/
Description:Under the NoDerivatives Creative Commons license one can take a work released under this license and re-distribute it, but it cannot be shared with others in adapted form, and credit must be provided to the author.
Licensing start date:10.08.2022

Secondary language

Language:English
Title:Bioactive substances from mango peels for the cosmetics industry
Abstract:Mango is considered the most farmed tropical fruit in the world. In addition to fresh fruit, you can process it into juices, nectars, concentrates, and marmalades, but during the processing itself remains a lot of waste material, such as peels, which are a serious environmental problem. However, flat peels are a rich source of polyphenol compounds, flavonoids, and vitamin C and have antimicrobial and antioxidant properties. For the implementation of the experimental part of the thesis, we prepared four samples, aqueous extract of dry mango peels, ethanolic extract of dry mango peels, aqueous extract of fresh mango peels, and ethanolic extract of fresh mango peels and analyzed using spectrophotometric analysis. The antimicrobial activity of isolates was assessed using Kirby-Bauer and microdilution Method. By measuring the inhibition zone, the Kirby – Bauer method determined the inhibitory properties of isolates on the growth of various micro-organisms, namely Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and fungi (Candida albicans and Aspergillus brasiliensis).With microdilution method only to determined the antimicrobial activity of isolates on bacterial growth, as previously using the Kirby-Bauer method achieved only successful inhibition of bacterial growth. Using the microdilution method, we determined the minimum inhibitory concentration of isolates and monitored the development of the bacterium. Spectrophotometric analyses of samples confirmed higher total phenol content, proanthocyanidin, and the higher antioxidant activity of ethanol extracts compared to aqueous extracts. From the measured inhibition zones, the Kirby-Bauer method demonstrated antimicrobial activity on the growth of Gram-positive and Gram-negative bacteria, while the samples tested did not inhibit the growth of fungi. The results of the microdilution method showed that antimicrobial properties expressed in Gram-positive bacteria compared to Gram-positive bacteria compared to Gram-negative. Based on the results obtained, we have demonstrated that manga peels are a rich source of bioactive agents, including antimicrobial and antioxidant activity. Therefore, in the future, it can be used in various industries such as cosmetics, food, and pharmaceuticals. Their further use could reduce the amount of waste material generated during the recovery of mangos, thus reducing the environmental impact of waste.
Keywords:mango peels, microorganisms, bioactive agents, antimicrobial, minimum inhibitory concentration


Comments

Leave comment

You must log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back
Logos of partners University of Maribor University of Ljubljana University of Primorska University of Nova Gorica