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Title:Stabilnost lizocima pri ekstremnih pogojih : diplomsko delo visokošolskega strokovnega študijskega programa I. stopnje
Authors:ID Dolinšek, Lucija (Author)
ID Leitgeb, Maja (Mentor) More about this mentor... New window
ID Primožič, Mateja (Comentor)
Files:.pdf VS_Dolinsek_Lucija_2021.pdf (2,22 MB)
MD5: 1493215A0A11CFAF5E3B2AA236369D6B
PID: 20.500.12556/dkum/1dcda873-8615-4942-93f7-fe1ea87e904b
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:V diplomskem delu smo preučevali vpliv SC CO2 na aktivnost prostega in imobiliziranega encima lizocim ob spreminjanju različnih parametrov, kot so tlak, temperatura in čas izpostavitve. Za primerjavo smo proučili tudi vpliv temperature in časa izpostavitve na stabilnost obeh oblik encima pri atmosferskem tlaku. Analizirali smo prosti in imobilizirani encim brez nosilca v obliki CLEAs. Namen diplomske naloge je bilo določiti optimalne pogoje za izpostavitev prostega encima in CLEAs v SC CO2 z morebitno hiperaktivacijo encima. Koncentracijo celokupnih proteinov smo določili z Bradfordovo metodo. S pomočjo UV-Vis spektrofotometra smo prostemu in imobiliziranemu encimu določili prisotnost celokupnih proteinov in aktivnost lizocima. Delež vode v vzorcih smo proučevali s pomočjo tehtanja mikrocentifugirk z vzorcem, in sicer pred in po izpostavitvi v SC CO2. Rezultati prikazujejo ugoden vpliv SC CO2 na aktivnost prostega in imobiliziranega encima, saj se je aktivnost obeh encimov po izpostavitvi v SC CO2 zvišala. Ugotovili smo, da je imobiliziran encim bolj stabilen kot prosti encim, kar se še posebej vidi pri ekstremnih pogojih (višja temperatura in tlak izpostavitve) in daljšem času izpostavitve v SC CO2. Z višanjem temperature in tlaka izpostavitve je prišlo do upada deleža vode v CLEAs.
Keywords:lizocim, superkritični ogljikov dioksid, zamreženi encimski skupki, aktivnost in stabilnost lizocima
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[L. Dolinšek]
Year of publishing:2021
Number of pages:IX, 44 str.
PID:20.500.12556/DKUM-79403 New window
UDC:544.032.1:577.15(043.2)
COBISS.SI-ID:72407043 New window
Publication date in DKUM:26.07.2021
Views:1413
Downloads:162
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
:
DOLINŠEK, Lucija, 2021, Stabilnost lizocima pri ekstremnih pogojih : diplomsko delo visokošolskega strokovnega študijskega programa I. stopnje [online]. Bachelor’s thesis. Maribor : L. Dolinšek. [Accessed 23 January 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=79403
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:24.06.2021

Secondary language

Language:English
Title:Stability of lysozyme under extreme conditions
Abstract:In this thesis, we studied the influence of SC CO2 on the activity of free and immobilized enzyme Lysozyme by changing various parameters such as pressure, temperature, and exposure time. For comparison, we also studied the effect of temperature and exposure time on the stability of both forms of the enzyme at atmospheric pressure. Free and immobilized carrier-free enzyme in the form of CLEAs was analysed. The purpose of the thesis was to determine the optimal conditions for exposure of free enzyme and CLEAs in SC CO2 with possible hyperactivation of the enzyme. The concentration of total proteins was determined by the Bradford method. Using an UV-Vis spectrophotometer, the presence of total proteins and lysozyme activity was determined fort the free and immobilized enzyme. The water content in the samples was studied by weighing the sample microcentrifuges tubes before and after exposure to SC CO2. The results show a beneficial effect of SC CO2 on the activity of the free and immobilized enzyme, as the activity of both enzymes increased after exposure to SC CO2. We found that the immobilized enzyme is more stable than the free enzyme, which is especially seen under extreme conditions (higher temperature and pressure exposure) and longer exposure time in SC CO2. As the temperature and pressure exposure increased, the proportion of water in the CLEAs decreased.
Keywords:Lysozyme, supercritical carbon dioxide, cross-linked enzyme aggregates, lysozyme activity and stability


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