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Title:Kontrolirano sproščanje febuksostata in določitev koncentracije v serumu in urinu : diplomsko delo univerzitetnega študijskega programa I. stopnje
Authors:ID Kouter, Marjetka (Author)
ID Kotnik, Petra (Mentor) More about this mentor... New window
ID Knez Marevci, Maša (Comentor)
Files:.pdf UN_Kouter_Marjetka_2020.pdf (2,79 MB)
MD5: CE08BE3DC7048C778DF9D1B8E0F095DF
PID: 20.500.12556/dkum/e07a11a7-3bc4-4737-9ed5-d8102574405b
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Febuksostat, zdravilo za zaviranje nastajanja sečne kisline, preprečuje napade putike. Protin, domače imenovan putika, je obolenje, ki predstavlja najpogostejši vnetni artitis pri moških, starejših od 40 let. V diplomskem delu smo v simulacijskih raztopinah (0,1 M HCl, fosfatni pufer s pH = 7,41 in deionizirana voda) kontrolno sproščali aktivno učinkovino febuksostat z metodo USP 1, ter določevali koncentracijo v različnih raztopinah, serumu in urinu. Za kvantifikacijo febuksostata sta bili uporabljeni občutlivi in specifični kromatografski metodi: tankoplastna kromatografija visoke ločljivosti (HPTLC) in tekočinska kromatografija z masno spektrometrijo (LC-MS/MS). Spreminjanju koncentracije febuksostata smo sledili z uporabo stresnih testov, staranja raztopin in kontroliranega raztapljanja v različnih simulacijskih raztopinah. Analizni metodi HPTLC in LC-MS/MS sta bili pripravljeni za določevanje koncentracije febuksostata v serumu in urinu. Najvišja vsebnost FEB po 24 h raztapljanja je bila zaznana v fosfatnem pufru. Z višanjem pH vrednosti topil smo ugotovili pozitiven vpliv na topnost FEB. S pomočjo stresnih testov smo določili degradacijo aktivne učinkovine. Najvišja degradacija se je pojavila pri uporabi 1M NaOH, kjer je po 48 h ostalo le še 0,03 % začetne mase FEB. Shranjevanje raztopin FEB pri višjih temperaturah povzroča staranje in posledično zmanjševanje koncentracije.
Keywords:febuksostat, kontrolirano raztapljanje, stresni testi, HPTLC, LC-MS/MS
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[M. Kouter]
Year of publishing:2020
Number of pages:IX, 29 str.
PID:20.500.12556/DKUM-77027 New window
UDC:544.351-145.1(043.2)
COBISS.SI-ID:31899139 New window
NUK URN:URN:SI:UM:DK:SCJRHM9L
Publication date in DKUM:08.10.2020
Views:758
Downloads:122
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
:
KOUTER, Marjetka, 2020, Kontrolirano sproščanje febuksostata in določitev koncentracije v serumu in urinu : diplomsko delo univerzitetnega študijskega programa I. stopnje [online]. Bachelor’s thesis. Maribor : M. Kouter. [Accessed 23 January 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=77027
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:11.08.2020

Secondary language

Language:English
Title:Controlled release of febuxostat and determination of serum and urine concentrations
Abstract:Febuxostat, a drug for inhibiting uric acid production, prevents gout attacks. Gout is a common condition representing the most frequent inflammatory arthritis occurring in men aged over 40. In our study, active ingredient febuxostat was released by the USP 1 method in simulation solutions (0.1 M HCl, phosphate buffer with pH = 7.4 and deionised water), and the concentration in several solutions, serum and urine was determined. For febuxostat quantification were used two sensitive and specific chromatographic methods: high- performance thin-layer chromatography (HPTLC) and liquid chromatography-mass spectrometry (LC-MS/MS). Changes in febuxostat concentration were monitored using stress tests, stability tests, and controlled dissolution in various simulation solutions. HPTLC and LC-MS/MS analytical methods were prepared to determine concentration of FEB in serum and urine. The highest FEB content after 24 h of dissolution was detected in phosphate buffer. By increasing the pH of the solvents, we found a positive effect on the solubility of FEB. Degradation of the active ingredient was determined using stress tests. The highest degradation occurred with the use of 1M NaOH, where only 0.03 % of the initial mass remained after 48 h. Storage of FEB solutions at higher temperatures causes aging and reduction in concentration.
Keywords:Febuxostat, controlled dissolution, stress testing, HPTLC, LC-MS/MS


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