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Title:Določanje težkih kovin v morski hrani z ICP-OES : magistrsko delo
Authors:ID Mulec, Monika (Author)
ID Finšgar, Matjaž (Mentor) More about this mentor... New window
ID Maver, Uroš (Comentor)
Files:.pdf MAG_Mulec_Monika_2020.pdf (1,46 MB)
MD5: A6018EED72A03E2D49CE0763A984EFF7
PID: 20.500.12556/dkum/1120494e-e252-407c-979e-bd2eb42f6805
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:V magistrskem delu smo določali prisotnost težkih kovin v vzorcih rib (iverka, skuša, morski list), školjk (klapavica) in alg. Izvedli smo delno validacijo analizne metode za določanje arzena (As), barija (Ba), kadmija (Cd), kroma (Cr), mangana (Mn), niklja (Ni), svinca (Pb) in stroncija (Sr) z uporabo induktivno sklopljene plazme z optično emisijsko spektrometrijo (ICP-OES). Določili smo mejo zaznavnosti (LOD), mejo določljivosti (LOQ), linearnost metode, natančnost kot ponovljivost metode in točnost metode. Z izračunom kvadrata korelacijskega koeficienta (r2) smo ovrednotili linearnost metode, ki je bila zadovoljiva za vse merjene analite (r2 ≥ 0,9990). Z izračunom relativnega standardnega odmika (RSD) meritev raztopin standardov smo ovrednotili natančnost kot ponovljivost metode, ki je bila zadovoljiva za vse merjene analite (RSD ≤ 20,0 %). Z izračunom izkoristka smo ovrednotili točnost metode, ki je bila zadovoljiva za vse merjene analite (od 80,0 % do 120,0 %). Realne vzorce smo pripravili s kislinskim razklopom (65-odstotna raztopina HNO3) z uporabo mikrovalovne pečice. Koncentracija As je v vzorcu iverke presegla koncentracijsko območje referenčnega odmerka (BMDL01), določenega s strani znanstvenega odbora za onesnaževalce v prehranjevalni verigi (CONTAM). V vzorcu skuše je bila koncentracija Cd nad mejno vrednostjo, določeno s strani Evropske komisije (EUC). Vsebnost Ba, Cr, Mn, Ni in Sr v realnih vzorcih ni presegla mejnih vrednosti ali pa mejne vrednosti niso predpisane. Pb v realnih vzorcih nismo določili.
Keywords:ICP-OES, težke kovine, kovine v ribah, kovine v školjkah, kovine v algah
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[M. Mulec]
Year of publishing:2020
Number of pages:X, 37 str.
PID:20.500.12556/DKUM-75958 New window
UDC:543.632.495:637.56(043.2)
COBISS.SI-ID:34228995 New window
NUK URN:URN:SI:UM:DK:8XDRRPLR
Publication date in DKUM:23.10.2020
Views:1375
Downloads:190
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
:
MULEC, Monika, 2020, Določanje težkih kovin v morski hrani z ICP-OES : magistrsko delo [online]. Master’s thesis. Maribor : M. Mulec. [Accessed 27 April 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=75958
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:21.03.2020

Secondary language

Language:English
Title:Determination of heavy metals in seafood by ICP-OES
Abstract:In this master thesis the presence of heavy metals in samples of fish (flounder, mackerel, sole), shellfish (mussels), and algae is determined. A partial validation of the analytical method for the determination of arsenic (As), barium (Ba), cadmium (Cd), chromium (Cr), manganese (Mn), nickel (Ni), lead (Pb), and strontium (Sr) was carried out using inductively coupled plasma with optical emission spectrometry (ICP-OES). We determined the limit of detection (LOD), the limit of quantification (LOQ), the linearity of the method, the precision as repeatability of the method, and the accuracy of the method. We evaluated the linearity of the method by calculating the square of the correlation coefficient, and it was satisfactory for all measured analytes (r2 ≥ 0.9990). We evaluated the precision as the repeatability of the method by calculating the relative standard deviation (RSD) of the measurements of standard solutions, and it was satisfactory for all measured analytes (RSD ≤ 20.0 %). The accuracy of the method was evaluated by recovery calculation, and was satisfactory (between 80.0 % and 120.0 %) for all the analytes measured. The real samples were prepared by microwave-assisted acid digestion (65% HNO3). The concentration of As in the flounder sample exceeded the concentration range of the benchmark dose (BMDL01) set by the Panel on Contaminants in the food chain (CONTAM). In the mackerel sample, the Cd concentration was above the limit value set by the European Commission (EUC). The content of Ba, Cr, Mn, Ni, and Sr in real samples did not exceed the limit values, or limit values are not established. Pb was found in real samples.
Keywords:ICP-OES, heavy metals, metals in fish, metals in shellfish, metals in algae


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