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Title:Antimikrobna učinkovitost Punica granatum L. na Escherichia coli : diplomsko delo visokošolskega strokovnega študijskega programa I. stopnje
Authors:ID Peklar, Anže (Author)
ID Leitgeb, Maja (Mentor) More about this mentor... New window
ID Primožič, Mateja (Comentor)
Files:.pdf VS_Peklar_Anze_2019.pdf (1,93 MB)
MD5: 8D83BE02BE1A886471966388E6FF2675
PID: 20.500.12556/dkum/d15a0ef8-1eed-42c4-b3dd-8824ddf6ed4f
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Namen diplomske naloge je pridobiti učinkovine iz različnih delov Punica granatum L. in proučiti njihov inhibitorni učinek na rast mikrobnih celic Escherichia coli ter določiti vsebnost totalnih fenolov in vitamina C v vzorcih. S klasično vodno ekstrakcijo in Soxhletovo ekstrakcijo z etanolom smo pridobili učinkovine iz svežih in liofiliziranih semen, membrane in eksokarpa Punica granatum L. Proučili smo inhibitorni učinek ekstraktov na rast mikrobne kulture E. coli z disk difuzijsko metodo ter določili vsebnost totalnih fenolov in vitamina C v vzorcih. Ugotovili smo, da se v celotnem sadežu Punica granatum L. nahajajo totalni fenoli, vitamin C in antimkrobne spojine, ki zavirajo rast bakterijske kulture E. coli. Koncentracije naštetih spojin so najvišje v membrani in eksokarpu Punica granatum L., saj smo v njihovih ekstraktih določili najvišjo vsebnost totalnih fenolov in vitamina C in omenjena dela Punica granatum L. izkazujeta tudi največji inhibitorni učinek na rast E. coli. Najmanj totalnih fenolov in vitamina C se nahaja v ekstraktih semen.
Keywords:Punica granatum L., antimikrobne lastnosti, totalni fenoli, vitamin C, inhibicija, bakterije, sekundarni metaboliti
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[A. Peklar]
Year of publishing:2019
Number of pages:IX, 29 str.
PID:20.500.12556/DKUM-74802 New window
UDC:[604.4:615.33]:582.772.1(043.2)
COBISS.SI-ID:22676758 New window
NUK URN:URN:SI:UM:DK:XEAIBLFT
Publication date in DKUM:10.10.2019
Views:1831
Downloads:91
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
:
PEKLAR, Anže, 2019, Antimikrobna učinkovitost  Punica granatum L.  na  Escherichia coli : diplomsko delo visokošolskega strokovnega študijskega programa I. stopnje [online]. Bachelor’s thesis. Maribor : A. Peklar. [Accessed 8 April 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=74802
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Licences

License:CC BY-ND 4.0, Creative Commons Attribution-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nd/4.0/
Description:Under the NoDerivatives Creative Commons license one can take a work released under this license and re-distribute it, but it cannot be shared with others in adapted form, and credit must be provided to the author.
Licensing start date:07.09.2019

Secondary language

Language:English
Title:Antimicrobial efficiency of Punica granatum L. on Escherichia coli
Abstract:The purpose of this work was to obtain bioactive substances from different parts of pomegranate (Punica granatum L.) and to study their inhibitory properties on the growth of Escherichia coli microbial cells and the content of total phenols and vitamin C. With the use of classical aqueous extraction and Soxhlet extraction with ethanol we have extracted bioactive substances from fresh and lyophilized pomegranate seeds, membranes and exocarp. The extracts were then tested for their inhibitory properties on the growth of Escherichia coli microbial culture by disk diffusion method. They were also tested for their total phenol content and vitamin C content. The results indicated that one whole pomegranate fruit contains total phenols, vitamin C and antimicrobial compounds that inhibit the growth of Escherichia coli bacterial culture. The concentration of these compounds is highest in the pomegranate membrane and exocarp, and the lowest in pomegranate seeds.
Keywords:pomegranate, antimicrobial properties, total phenols, vitamin C, inhibition, bacteria, secondary metabolites


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