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Title:Vpliv sestave gojišča na aktivnost encimov iz Pleurotus ostreatus : diplomsko delo univerzitetnega študijskega programa I. stopnje
Authors:ID Konajzler, Anja (Author)
ID Leitgeb, Maja (Mentor) More about this mentor... New window
ID Primožič, Mateja (Comentor)
ID Kravanja, Gregor (Comentor)
Files:.pdf UN_Konajzler_Anja_2019.pdf (2,98 MB)
MD5: 2BBBB2BDE2DE7C73873C6EF0903A7C37
PID: 20.500.12556/dkum/0e239e73-158c-4efe-9107-5365638366dc
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:V diplomskem delu je opisano gojenje glive Pleurotus ostreatus na različnih gojiščih, ki so bila sestavljena iz mešanice pšeničnih otrobov ter sezonskega sadja in zelenjave. Proučevali smo vpliv sestave gojišča na prirast glive ter na aktivnost encimov (lakaze, celulaze in α-amilaze) v ekstraktih po 8 dneh gojenja glive. Razrast glive smo spremljali 8 dni in v določenih intervalih spremljali prirast oz. zmanjšanje biomase. Po 8 dneh smo ekstrahirali encime iz razrastlega P. ostreatus na izbranih gojiščih. Določili smo koncentracijo proteinov ter aktivnost in specifično aktivnost posameznih encimov (lakaze, celulaze in α-amilaze) v encimskih ekstraktih. Nato smo iz encimskih ekstraktov pripravili zamrežene encimske skupke (CLEAs). Določili smo učinkovitost imobilizacije, nato pa še aktivnost in specifično aktivnost za vse 3 encime v CLEAs. Zanimala nas je tudi stabilnost CLEAs, ki smo jo skladiščili pri 4°C. Preostalo aktivnost CLEAs smo zasledovali po 2 in 4 tednih skladiščenja pri 4°C.
Keywords:Pleurotus ostreatus, celulaza, lakaza, α-amilaza, CLEAs, stabilnost encimov
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[A. Konajzler]
Year of publishing:2019
Number of pages:X, 52 str.
PID:20.500.12556/DKUM-74357 New window
UDC:577.15:582.28(043.2)
COBISS.SI-ID:22682390 New window
NUK URN:URN:SI:UM:DK:4XTYPVKO
Publication date in DKUM:10.10.2019
Views:1847
Downloads:152
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
:
KONAJZLER, Anja, 2019, Vpliv sestave gojišča na aktivnost encimov iz  Pleurotus ostreatus : diplomsko delo univerzitetnega študijskega programa I. stopnje [online]. Bachelor’s thesis. Maribor : A. Konajzler. [Accessed 26 March 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=74357
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:25.08.2019

Secondary language

Language:English
Title:The influence of composition of the medium of enyzmes activity Pleurotus ostreatus
Abstract:The diploma work describe the cultovation of Pleurotus ostreatus on various residues of fruits and vegetables, in addition to wheat bran. The fungus growth was observed for 8 days and weight in between to measure the weight of the fungus. After 8 days, we did the enzyme extraction. The enzymes like cellulase, laccase and α-amylase were observed and the protein concentration was determined according to the Bradford method. Activity and specific activity in enzyme extract were also determined. Subsequently, cross-linked enzyme aggregates (CLEAs) were made. The immobilization efficiency was determined, and then the residual activity and residual specific activity for all 3 enzymes were measured. We were also interested in the stability of enzymes in CLEAs over time at 4°C, so we set cross-linked enzyme aggregates at 4°C and measured the enzyme activity after 2 and 4 weeks.
Keywords:Pleurotus ostreatus, cellulase, laccase, α-amylase, CLEAs, stability of enzymes


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