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Title:16S rRNA in situ hybridization followed by flow cytometry for rapid identification of acetic acid bacteria involved in submerged industrial vinegar production
Authors:ID Trček, Janja (Author)
ID Lipoglavšek, Luka (Author)
ID Avguštin, Gorazd (Author)
Files:.pdf Food_Technology_and_Biotechnology_2016_Trcek,_Lipoglavsek,_Avgustin_16S_rRNA_in_situ_Hybridization_Followed_by_Flow_Cytometry_for_Rapid.pdf (500,81 KB)
MD5: 7809705424A5B6A20D971CB67CD2D3E2
PID: 20.500.12556/dkum/7d57fe7f-ea23-45d0-9276-d7210ee370c2
 
URL http://www.ftb.com.hr/index.php/archives/156-volume-54-issue-no-1/1404-16s-rrna-in-situ-hybridization-followed-by-flow-cytometry-for-rapid-identifi-cation-of-acetic-acid-bacteria-involved-in-submerged-industrial-vinegar-production
 
Language:English
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:Acetic acid bacteria are involved in many biotechnological processes such as vitamin C, gluconic acid, miglitol or acetic acid production, and others. For a technologist trying to control the industrial process, the ability to follow the microbiological development of the process is thus of importance. During the past few years hybridization in a combination with flow cytometry has oft en been used for this purpose. Since vinegar is a liquid, it is an ideal matrix for flow cytometry analysis. In this work we have constructed a specific probe for highly acetic acid-resistant species of the acetic acid bacteria and a protocol for in situ hybridization, which in combination with flow cytometry enables direct monitoring of bacteria producing vinegar with >10 % of acetic acid. The approach was successfully applied for monitoring microbiota during industrial vinegar production.
Keywords:microbiology, acetic acid bacteria, flow cytometry
Publication status:Published
Publication version:Version of Record
Year of publishing:2016
Number of pages:str. 108-112
Numbering:Letn. 54, št. 1
PID:20.500.12556/DKUM-66997 New window
ISSN:1330-9862
UDC:579.66
ISSN on article:1330-9862
COBISS.SI-ID:22612232 New window
DOI:10.17113/ftb.54.01.16.4041 New window
NUK URN:URN:SI:UM:DK:QWDOJMCU
Publication date in DKUM:24.07.2017
Views:27785
Downloads:397
Metadata:XML DC-XML DC-RDF
Categories:Misc.
:
TRČEK, Janja, LIPOGLAVŠEK, Luka and AVGUŠTIN, Gorazd, 2016, 16S rRNA in situ hybridization followed by flow cytometry for rapid identification of acetic acid bacteria involved in submerged industrial vinegar production. Food Technology and Biotechnology [online]. 2016. Vol. 54, no. 1, p. 108–112. [Accessed 1 April 2025]. DOI 10.17113/ftb.54.01.16.4041. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=66997
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Record is a part of a journal

Title:Food Technology and Biotechnology
Shortened title:Food Technol. Biotechnol.
Publisher:Faculty of Food Technology and Biotechnology, University of Zagreb
ISSN:1330-9862
COBISS.SI-ID:40116737 New window

Licences

License:CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
Link:http://creativecommons.org/licenses/by-nc/4.0/
Description:A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.
Licensing start date:24.07.2017

Secondary language

Language:Slovenian
Keywords:mikrobiologija, ocetnokislinske bakterije, pretočna citometrija


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