| | SLO | ENG | Cookies and privacy

Bigger font | Smaller font
First page > Show document

Show document Help

Title:Encimi v likvorju
Authors:ID Kravos, Matej (Author)
ID Kralj, Grega (Author)
ID Malešič, Ivan (Author)
Files:.pdf Zdravniski_vestnik_2008_Kravos,_Kralj,_Malesic_ENCIMI_V_LIKVORJU.pdf (120,89 KB)
MD5: 83784D7D300679ED65A93AE5EFFD9505
 
URL http://vestnik.szd.si/index.php/ZdravVest/article/view/861
 
Language:Slovenian
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Abstract:Izhodišča: V Univerzitetnem kliničnem centru (UKC) Maribor smo za leto 2006 hoteli ugotoviti število punkcij in spekter naročenih preiskav s poudarkom na encimski diagnostiki v likvorju. Možgani so zelo bogati z glutamat-dehidrogenazo (GLDH). Ker v literaturi ni podatkov o njegovi aktivnosti v likvorju, smo poskušali ugotoviti, ali obstajajo merljive aktivnosti GLDH. Poškodbe celic centralnega živčnega sistema (CŽS) povzročijo sproščanje encimov in dvig njihove aktivnosti v likvorju enako kot povzročijo poškodbe drugih tkiv sproščanje encimov v zunajcelično tekočino. Krvno-likvorska pregrada je normalno neprepustna za številne encime krvi, zatougotovljene encimske aktivnosti v likvorju večinoma ne izvirajo iz krvi. GLDH je parenhimski encim in se nahaja v mitohondrijih vseh celic, v možganih pa v celicah nevronov in glije. Njegova znižana aktivnost v možganih lahko privede do degenerativnih okvar oziroma bolezni. Bolniki in metode: V UKC Maribor je bilo opravljenih 1241 lumbalnih punkcij. Določanje beljakovin in glukoze je bilo opravljeno v vseh vzorcih. Izvršene ni bilo nobene encimske preiskave v likvorju. Do sedaj smo na aktivnost GLDH analizirali 71 likvorjev. Encimske aktivnosti so variirale od 0-340 nkat/L. Samo v petih primerih nismo ugotovili aktivnosti GLDH. Raziskava še poteka. Rezultati: V večini analiziranih likvorjev (92,96%) smo ugotovili merljive aktivnosti GLDH.Največ (32,08%) jih je bilo od oseb, obolelih z multiplo sklerozo in pri vseh smo ugotovili merljivo aktivnost GLDH. Podatek še ni objavljen v strokovni literaturi. Zaključki: Ugotovitev merljivih aktivnosti GLDH v likvorju je pomembna glede na njegovo specifično vlogo v možganih. Nadaljnje raziskave bodo ugotovile, ali so tudi diagnostično značilne za določene bolezni CŽS.
Keywords:lumbalna punkcija, likvor, encimska diagnostika, glutamat-dehidrogenaza, glutamat, multipla skleroza
Publication status:Published
Publication version:Version of Record
Year of publishing:2008
Number of pages:str. 593-599
Numbering:Letn. 77, št. 9
PID:20.500.12556/DKUM-65262 New window
ISSN:1318-0347
UDC:616.8
ISSN on article:1318-0347
COBISS.SI-ID:24973785 New window
NUK URN:URN:SI:UM:DK:LC4PWKEK
Publication date in DKUM:27.03.2017
Views:2306
Downloads:168
Metadata:XML DC-XML DC-RDF
Categories:Misc.
:
KRAVOS, Matej, KRALJ, Grega and MALEŠIČ, Ivan, 2008, Encimi v likvorju. Zdravniški vestnik. glasilo Slovenskega zdravniškega društva [online]. 2008. Vol. 77, no. 9, p. 593–599. [Accessed 24 March 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=65262
Copy citation
  
Average score:
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
(0 votes)
Your score:Voting is allowed only for logged in users.
Share:Bookmark and Share


Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Record is a part of a journal

Title:Zdravniški vestnik. glasilo Slovenskega zdravniškega društva
Publisher:Slovensko zdravniško društvo
ISSN:1318-0347
COBISS.SI-ID:32893696 New window

Licences

License:CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
Link:http://creativecommons.org/licenses/by-nc/4.0/
Description:A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.
Licensing start date:27.03.2017

Secondary language

Language:English
Title:Cerebrospinal fluid enzymes
Abstract:Background: Our goal was to determine the actual amount of lumbar punctures andspectrum of analyses, particularly on enzyme diagnostics, ordered at the University Clinical Center Maribor in year 2006. As the brain is very rich on glutamate dehydrogenaze (GLDH) we also attempted to discover, if there is measurable GLDH activity in cerebrospinal fluid as there is no reliable data. Brain cellular damage involves enzyme release and increases their activity in liquor in the same way as the damages of other tissues causes the enzyme release in extracellular fluids. As the haemathoencephalic barrier is normallynonpermeable for enzymes in blood does their activity in cerebrospinalfluid (CSF) mostly not derive from it. Results: There were obtained 1241 lumbar punctures. Proteins and glucose were determined in all CSF samples. We did not determine any enzyme analyses in CSF. 71 CSF samples were analyzed for the presence of GLDH activity which varied form 0-340 nkat/L. Only in five CSF samples we did not find any GLDH activity. Research is still in progress. Conclusions: We determined the measurable GLDH activity in the majority (92.96%) of analysed cerebrospinal fluids. This is a completely new finding. Most of analyzed CSF (32.08%) belongs to patients withmultiple sclerosis and all had measurable GLDH activity. We conclude that determining of GLDH activity in CSF is an important tool for further investigations and probably an useful diagnostic help.
Keywords:lumbar puncture, cerebrospinal fluid, enzyme diagnostics, glutamate dehydrogenase, glutamate, multiple sclerosis


Comments

Leave comment

You must log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back
Logos of partners University of Maribor University of Ljubljana University of Primorska University of Nova Gorica