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Title:RAZVOJ HPLC ANALIZNE METODE ZA DOLOČANJE SPROŠČANJA ZDRAVILNIH UČINKOVIN IZ MEDICINSKIH OBLOG OB SOČASNEM UPOŠTEVANJU EROZIJE NOSILNEGA MATERIALA
Authors:ID Virant, Natalija (Author)
ID Kolar, Mitja (Mentor) More about this mentor... New window
ID Brodnjak-Vončina, Darinka (Comentor)
ID Stana-Kleinschek, Karin (Comentor)
Files:.pdf MAG_Virant_Natalija_2015.pdf (4,03 MB)
MD5: 52DE4DCFE25C49819A805AC30B2DA4CD
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Namen magistrskega dela je bil razviti in validirati HPLC analizno metodo za določanje sproščanja benzokaina iz nanovlaken. V ta namen smo pripravili elektropredena nanovlakna z benzokainom in optimizirali postopek elektropredenja iz celuloznega acetata (CA). Pri tem smo preučevali vpliv procesnih parametrov in parametrov predilne raztopine na potek elektropredenja. Dobljena nanovlakna smo okarakterizirali s SEM in FTIR analizo, določili smo tudi stopnjo nabrekanja in stični kot. Koncentracije benzokaina smo določali z uporabo kolone Supelcosil LC-18 (20 cm x 4,6 cm, 3µm) in UV-VIS detektorja pri valovni dolžini 285 nm. Kot optimalno mobilno fazo smo izbrali acetonitril:voda (60:40) pri pretoku 0,8 ml/min. Pri teh pogojih se je benzokain eluiral pri 4,26 min. Določili smo, da je razvita metoda natančna, točna in linearna v območju med 5 in 50 mg/l. Meja zaznavnosti (LOD) znaša 1,08 mg/l, meja določljivosti (LOQ) pa 3,27 mg/l. Gladka nanovlakna, s premerom med 400 in 900 nm, smo izpredli iz 17 ut. % CA v 85 % ocetni kislini. Optimalni procesni parametri elektropredenja so: napetost 75 kV in razdalja med elektrodama 160 mm. Optimalni predilni raztopini smo dodali 5 % benzokaina (glede na maso CA) in ta dodatek ni vplival na postopek elektropredenja.
Keywords:tekočinska kromatografija visoke ločljivosti (HPLC), validacija, elektropredenje, benzokain, kinetika sproščanja
Place of publishing:Maribor
Publisher:[N. Virant]
Year of publishing:2015
PID:20.500.12556/DKUM-54920 New window
UDC:543.544.5(043.2)
COBISS.SI-ID:19035670 New window
NUK URN:URN:SI:UM:DK:FIX4AOIV
Publication date in DKUM:22.10.2015
Views:4463
Downloads:317
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
:
VIRANT, Natalija, 2015, RAZVOJ HPLC ANALIZNE METODE ZA DOLOČANJE SPROŠČANJA ZDRAVILNIH UČINKOVIN IZ MEDICINSKIH OBLOG OB SOČASNEM UPOŠTEVANJU EROZIJE NOSILNEGA MATERIALA [online]. Master’s thesis. Maribor : N. Virant. [Accessed 22 March 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=54920
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Secondary language

Language:English
Title:DEVELOPMENT OF HPLC METHOD FOR DETERMINATION OF VARIOUS PHARMACEUTICAL COMPOUNDS AND STUDY OF SUPPORTING MATERIAL EROSION
Abstract:The aim of the master thesis was developed and validated HPLC analysis method for determination of benzocaine release from nanofibers. For this purpose we have prepared electrospun nanofibers with benzocaine and optimized electrospinning process of cellulose acetate (CA). We have studied the influence of solution parameters and process parameters of electrospinning. Electrospun nanofibers were characterized by SEM and FTIR analysis. Swelling behaviors and contact angle were also investigated. Concentrations of benzocaine were determined using Supelcosil LC-18 (20 cm x 4,6 cm, 3µm) column. The UV-VIS detector was set at 285 nm. The mobile phase consisted of acetonitrile:water (60:40) and used flow rate was 0,8 ml/min. Under these conditions benzocaine eluted at 4,26 min. We confirmed that method was precise, accurate and linear from 5 to 50 mg/l. The limits of detection (LOD) and quantification (LOQ) values were 1,08 mg/l and 3,27 mg/l, respectively. Smooth nanofibers with diameters ranging from 400 to 900 nm were electrospun from a 17 ut. % CA solution in 85 % acetic acid. Optimal electrospinning parameters are 160 mm distance between electrodes and 75 kV voltage. The addition of 5 % benzocaine (based on the weight of CA) in the spinning formulation did not affect the fiber formation.
Keywords:high performance liquid chromatography (HPLC), validation, electrospinning, benzocaine, drug release kinetics


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