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Title:Novejši biooznačevalci in spremljanje znotrajceličnih signalnih poti pri avtoimunskih boleznih
Authors:ID Goropevšek, Aleš (Author)
ID Homšak, Evgenija (Author)
ID Gorenjak, Maksimiljan (Author)
ID Malešič, Ivan (Author)
ID Krajnc, Ivan (Author)
ID Cencič, Avrelija (Author)
Files:.pdf Zdravniski_vestnik_2008_Goropevsek_et_al._Novejsi_biooznacevalci_in_spremljanje_znotrajcelicnih_signalnih_poti_pri_avtoimunskih_boleznih.pdf (142,68 KB)
MD5: FB0E2106B7D01CC963D54E67D3F69801
 
URL http://vestnik.szd.si/index.php/ZdravVest/article/view/864
 
Language:Slovenian
Work type:Scientific work
Typology:1.02 - Review Article
Organization:MF - Faculty of Medicine
Abstract:Izhodišča: Avtoimunske in kronične vnetne bolezni se še vedno pogosto zdravijo le z nespecifično imunosupresijo, ki ne prinaša ozdravitve. Spoznanje, da so citokini TNF in IFN-alfa bistveni v patogenezi bolezni, kot sta revmatoidni artritis in sistemski lupus eritematozus, pomeni napredek v razumevanju avtoimunskih bolezni. Znotrajcelične signalne poti, ki se aktivirajo kot odgovor na te klinično pomembne citokine, prenašajo signale s kinazno fosforilacijo proteinov in so bistvene za delovanje celic imunskega sistema. Malo je znanega o spremembah teh signalnih poti pri avtoimunskih boleznih. Nedavne klinične raziskave so pokazale, da se spoznanja iz živalskih modelov ne morejo neposredno prenesti na človeka. Za spremljanje aktivnosti bolezni in napoved odziva na novejše zdravljenje je potrebno razviti nova orodja za spremljanje humanega imunskega odziva. Obetajoče orodje v prihodnosti so novo odkriti biomarkerji. Še več si obetamo od pristopov, ki temeljijo na spremljanju signalnih poti na celični ravni. Zaključki: Razviti so bili biokemični analizni sistemi, ki temeljijo na pretočni citometriji in omogočajo profiliranje kinaz in fosfoproteinov na ravni posameznih celic. To bo omogočilo študije signalnih poti pri avtoimunskih in kroničnih vnetnih boleznih, saj so analizni sistemi prilagojeni prav celicam imunskega sistema, npr. v periferni krvi. Prvi rezultati kažejo značilne fosfo-signature citokinov (interferonov) v imunskih celicah bolnikov s SLE. Možnost spremljanja signalnih poti na celični ravni lahko prinese razvoj novih diagnostičnih možnosti, predvsem za spremljanje aktivnosti bolezni in vodenja zdravljenja. Rezultati študij pa lahko nakažejo tudi nove tarče, bolj specifičnega in manj toksičnega, zdravljenja z inhibitorji kinaz.
Keywords:SLE, pretočna citometrija, citokinske signalne poti, IFN-alfa
Publication status:Published
Publication version:Version of Record
Year of publishing:2008
Number of pages:str. 615-622
Numbering:Letn. 77, št. 9
PID:20.500.12556/DKUM-52606 New window
ISSN:1318-0347
UDC:616-097
ISSN on article:1318-0347
COBISS.SI-ID:3096895 New window
NUK URN:URN:SI:UM:DK:HIRZRW63
Publication date in DKUM:10.07.2015
Views:2328
Downloads:109
Metadata:XML DC-XML DC-RDF
Categories:Misc.
:
GOROPEVŠEK, Aleš, HOMŠAK, Evgenija, GORENJAK, Maksimiljan, MALEŠIČ, Ivan, KRAJNC, Ivan and CENCIČ, Avrelija, 2008, Novejši biooznačevalci in spremljanje znotrajceličnih signalnih poti pri avtoimunskih boleznih. Zdravniški vestnik. glasilo Slovenskega zdravniškega društva [online]. 2008. Vol. 77, no. 9, p. 615–622. [Accessed 2 April 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=52606
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Record is a part of a journal

Title:Zdravniški vestnik. glasilo Slovenskega zdravniškega društva
Publisher:Slovensko zdravniško društvo
ISSN:1318-0347
COBISS.SI-ID:32893696 New window

Licences

License:CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
Link:http://creativecommons.org/licenses/by-nc/4.0/
Description:A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.
Licensing start date:10.07.2015

Secondary language

Language:English
Title:New biomarkers and monitoring intracellular signaling pathways in autoimmune diseases
Abstract:Background: Almost all current therapeutic concepts in many autoimmune and chronic inflammatory diseases are based on the systemic suppression of immune functions and are not curative. Identification of cytokines TNF and IFN-alpha as major factors in the pathogenesis of diseases such as rheumatoid arthritis and systemic lupus erythematosus (SLE) represent a substantial improvement in understanding of autoimmune diseases. Intracellular signalling pathways that are activated in response to those clinically relevant cytokines, mediate signals through kinase phosphorylation of proteins and are at the core of immune cell function. However, little is known about their changes in autoimmune disease states. Recent trials emphasized the importance of directl yassessing the human immune responses, and that not all of what we learn for example in the mouse can be directly translated to humans. Thus, there is a need for the development of tools and assays to directly assess the human immune system, and to predict its responses to novel therapeutic entities. Newly discovered biomarkers represent promising tools. Even more promising are approaches, that are based on monitoring immune signaling on the single cell level. Conclusions: A series of assay systems for flow cytometric-based biochemical analysis at the single-cell level for kinase and phosphoprotein profiling have been developed. This will give us opportunity to study signal pathways also in autoimmune and chronic inflammatory diseases, as the analysing systems are adapted to immunocytes for example in peripheral blood. First results show characteristic phospho-signature of cytokines (interferons) in immune cells from SLE patients. Monitoring signaling pathways on the single cell level can lead to developments in new diagnostic tools, especially in monitoring of disease activity. Results can also identify new targets of more specific and less toxic therapy with kinase inhibitors.
Keywords:SLE, flow cytometry, cytokine signalling, IFN-alpha


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