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Title:
DOLOČANJE VSEBNOSTI BETULINA IN BETULINSKE KISLINE V LUBJU BREZE
Authors:
ID
Staniša, Robi
(Author)
ID
Knez, Željko
(Mentor)
More about this mentor...
ID
Škerget, Mojca
(Comentor)
Files:
UNI_Stanisa_Robi_2010.pdf
(2,64 MB)
MD5: D1C21F82F44AE994488159C9F64832A3
PID:
20.500.12556/dkum/a3aca2a8-152e-41aa-9e55-de616b90b19e
Language:
Slovenian
Work type:
Undergraduate thesis
Organization:
FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:
Eni največjih virov prostih radikalov oz. največjih oksidantov so kisik, kajenje, UV-sevanje in onesnaženost, ki prihaja iz okolja. O antioksidantih je bilo narejenih ogromno raziskav in študij, saj so bistvenega pomena za naše zdravje in vitalnost, imajo zavirajoč vpliv na rakave celice, staranje in bolezni nasploh.(1) Betulin in betulinska kislina iz brezinega lubja nas varujeta pred škodljivimi prostimi radikali, saj sodita med antioksidante oziroma lovilce prostih radikalov.(2) V diplomski nalogi smo izolirali betulin in betulinsko kislino iz lubja breze s superkritično ekstrakcijo s CO2. Poskuse smo opravili pri različnih temperaturah, tlakih in času, da smo ugotovili, pri katerih pogojih je izkoristek najboljši. Najboljši izkoristek smo dobili pri ekstrakciji, ki je potekala 2 uri pri 80°C in tlaku 300 bar. K superkritičnemu CO2 smo dodali tudi kotopili metanol in etanol ter primerjali rezultate. Dobljene produkte smo nato analizirali s HPLC, kjer smo predhodno s standardom betulina in betulinske kisline določili vrednosti na kromatogramih pri valovni dolžini 215 nm in z računalniško integracijo ploščin izračunali maso aktivnih komponent na gram ekstrakta pri različnih pogojih in topilih. Določili smo tudi kinetiko poteka ekstrakcije.
Keywords:
betulin
,
betulinska kislina
,
ekstrakcija
,
breza
,
superkritični fluid
Place of publishing:
Maribor
Publisher:
[R. Staniša]
Year of publishing:
2010
PID:
20.500.12556/DKUM-17121
UDC:
665.5.061(043.2)
COBISS.SI-ID:
15002390
NUK URN:
URN:SI:UM:DK:5ZGKZ0NU
Publication date in DKUM:
23.12.2010
Views:
2835
Downloads:
193
Metadata:
Categories:
KTFMB - FKKT
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:
STANIŠA, Robi, 2010,
DOLOČANJE VSEBNOSTI BETULINA IN BETULINSKE KISLINE V LUBJU BREZE
[online]. Bachelor’s thesis. Maribor : R. Staniša. [Accessed 21 January 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=17121
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Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.
Secondary language
Language:
English
Title:
OBTAINING CONTAIN OF BETULIN AND BETULINIC ACID FROM BIRCH BARK
Abstract:
Some of the biggest free-floating radicals i.e. biggest oxidants are oxygen, smoking, UV-radiation and pollution coming from environment. Many researches and studies on antioxidants have been made, and they are of a crucial importance to our health and vitality, and have obstructive influence upon cancer cells, aging and deseases in general. Betulin and Betulinic acid from birch-tree bark, the cathcers of free radiccals, protect us from harming free-floating radicals, whic belong to antioxidants. In diploma, betulin and betulin-ic acid from bark were isolated by means of extraction with CO2. Experiments were made at different temperatures, pressures and at different times, to find out the influence of conditions on the extraction yield. The best result was obtained when extraction was performed at 80 °C under 300 bar pressure. Co-solvents methane and ethane were added to supercritical CO2 and results were compared. Resulting products were analysed with HPLC, by means of standards betulin and betulinic acid, for which the chromatograms were measured at 215 nm wave length, and with the help of the computer integration of areas, the weight of active components per g of extract were calculated. Kinetic of extraction process was also determined experimentaly.
Keywords:
betulin
,
betulinic acid
,
extraction
,
birch
,
supercritical fluid
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