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Title:Microbiota in vitro modulated with polyphenols shows decreased colonization resistance against Clostridioides difficile but can neutralize cytotoxicity
Authors:ID Mahnič, Aleksander (Author)
ID Auchtung, Jennifer (Author)
ID Poklar Ulrih, Nataša (Author)
ID Britton, Robert A. (Author)
ID Rupnik, Maja (Author)
Files:.pdf Mahnic-2020-Microbiota__i_in_vitro__i__modulat.pdf (1,67 MB)
MD5: 2CC00F4AB632A741987E0AB2C703F5C0
 
URL https://doi.org/10.1038/s41598-020-65253-0
 
Language:English
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Abstract:While the knowledge on gut microbiota - C. difficile interactions has improved over the years, the understanding of the underlying mechanisms providing colonization resistance as well as preventative measures against the infection remain incomplete. In this study the antibiotic clindamycin and polyphenol extracts from pomegranate and blueberries were used individually and in combination to modulate fecal microbial communities in minibioreactor arrays (MBRA). Modulated communities were inoculated with C. difficile (ribotype 027). Subsequent 7-day periodical monitoring included evaluation of C. difficile growth and activity of toxins TcdA and TcdB as well as analysis of MBRA bacterial community structure (V3V4 16%S metagenomics). Polyphenols affected multiple commensal bacterial groups and showed different synergistic and antagonistic effects in combination with clindamycin. Exposure to either clindamycin or polyphenols led to the loss of colonization resistance against C. difficile. The successful growth of C. difficile was most significantly correlated with the decrease in Collinsella and Lachnospiraceae. Additionally, we demonstrated that Clostridium sporogenes decreased the activity of both C. difficile toxins TcdA and TcdB. The feature was shown to be common among distinct C. sporogenes strains and could potentially be applicable as a non-antibiotic agent for the alleviation of C. difficile infection.
Keywords:microbiota in vitro, Clostridioides difficile, resistance, cytotoxicity
Publication status:Published
Publication version:Version of Record
Submitted for review:13.01.2020
Article acceptance date:21.03.2020
Publication date:20.05.2020
Publisher:Nature Publishing Group
Year of publishing:2020
Number of pages:Str. 1-11
Numbering:Letn. 10, št. 8358
PID:20.500.12556/DKUM-91707 New window
UDC:579.852.13
ISSN on article:2045-2322
COBISS.SI-ID:16205827 New window
DOI:10.1038/s41598-020-65253-0 New window
Publication date in DKUM:29.01.2025
Views:0
Downloads:3
Metadata:XML DC-XML DC-RDF
Categories:Misc.
:
MAHNIČ, Aleksander, AUCHTUNG, Jennifer, POKLAR ULRIH, Nataša, BRITTON, Robert A. and RUPNIK, Maja, 2020, Microbiota in vitro modulated with polyphenols shows decreased colonization resistance against Clostridioides difficile but can neutralize cytotoxicity. Scientific reports [online]. 2020. Vol. 10, no. 8358, p. 1–11. [Accessed 7 April 2025]. DOI 10.1038/s41598-020-65253-0. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=91707
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Record is a part of a journal

Title:Scientific reports
Shortened title:Sci. rep.
Publisher:Nature Publishing Group
ISSN:2045-2322
COBISS.SI-ID:18727432 New window

Document is financed by a project

Funder:ARRS - Slovenian Research Agency
Project number:P3-0387
Name:Črevesna mikrobiota - vloga v zdravju in pri boleznih
Funder:Other - Other funder or multiple funders
Project number:FEMS-RG-2016-0048

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:20.05.2020

Secondary language

Language:Slovenian
Keywords:mikrobiota, C. difficile, odpornost, citotoksičnost


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