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Title:Porozne membrane na osnovi poli(etilen glikola) kot matrike za rast celic
Authors:ID Skušek, Nika (Author)
ID Krajnc, Peter (Mentor) More about this mentor... New window
ID Lipovšek, Saška (Comentor)
ID Dariš, Barbara (Comentor)
Files:.pdf MAG_Skusek_Nika_2024.pdf (4,54 MB)
MD5: 9FD93BAACCD624EB59205BEDE45E5DBD
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Zdravljenje poškodb ali s starostjo povezanih bolezni predstavlja velik izziv v trenutni klinični praksi. Transplantati, ki se pri tem uporabljajo, imajo številne pomanjkljivosti kot je razpoložljivost donorskih tkiv, invazivnost kirurških posegov in možnost okužbe ali zavrnitve tkiva. Zaradi tega se na biomedicinskem področju vse bolj uveljavlja tkivno inženirstvo, ki temelji na uporabi matrik iz različnih biomaterialov. Za sintezo le-teh se uporabljajo tudi porozni polimerni materiali pripravljeni z uporabo emulzij z visokim deležem notranje faze. V magistrskem delu smo sintetizirali porozne poliHIPE membrane na osnovi poli(etilen glikol) diakrilata in poli(etilen glikol) dimetakrilata v kombinaciji z 2-hidroksietil metakrilatom. Z namenom izboljšanja celične proliferacije preko povečanja povprečnih premerov por smo spreminjali parametre priprave emulzij. Spreminjali smo koncentracijo surfaktanta, temperaturo, hitrost mešanja in formulacijam dodajali različna topila. Dobljeno morfologijo smo preučili z uporabo vrstične elektronske mikroskopije (SEM) in na podlagi dobljenih slik izmerili velikosti primarnih, sekundarnih in površinskih por, ki so značilne za poliHIPE materiale. Izbrane vzorce smo uporabili za testiranje metabolne aktivnosti celičnih kultur WM-266-4 (ATCC®) in NHEK (PromoCell). Na podlagi karakterističnih signalov na FTIR spektrih smo preverili uspešnost sinteze. Nenazadnje smo določili še celokupno gostoto in poroznost s pomočjo GeoPyc analizatorja gostote ter lastnosti razgradnje s termogravimetrično analizo (TGA).
Keywords:poliHIPE, tkivno inženirstvo, poli(etilen glikol), metabolna aktivnost celic, PEGDMA/PEGDA
Place of publishing:Maribor
Year of publishing:2024
PID:20.500.12556/DKUM-89636 New window
Publication date in DKUM:21.08.2024
Views:106
Downloads:30
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
:
SKUŠEK, Nika, 2024, Porozne membrane na osnovi poli(etilen glikola) kot matrike za rast celic [online]. Master’s thesis. Maribor. [Accessed 9 April 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=89636
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:30.07.2024

Secondary language

Language:English
Title:Poly(Ethylene glycol) based porous membranes as cell growth scaffolds
Abstract:The treatment of injuries or age-related diseases poses a major challenge in current clinical practice. The transplants used in this process have several disadvantages, such as the availability of donor tissues, the invasiveness of surgical procedures and the possibility of an infection or a rejection of the tissue. For this reason, tissue engineering based on the use of matrices from various biomaterials is becoming favourable in the biomedical field. To prepare such (porous) materials high internal phase emulsions were used. In the master's thesis, porous polyHIPE membranes based on poly(ethylene glycol) diacrylate and poly(ethylene glycol) dimethacrylate in combination with 2-hydroxyethyl methacrylate were synthesized. In order to improve cell proliferation by increasing the average pore diameters, the parameters of the emulsion preparation were varied. Different surfactant concentrations, preparation temperature, mixing rates and different solvents were used to alter the emulsions. The obtained morphology was studied using scanning electron microscopy (SEM) and based on the obtained images, the sizes of the primary, the secondary and the surface pores were measured. The prepared samples were used to test the metabolic activity of cell cultures WM-266-4 (ATCC®) and NHEK (PromoCell). Based on characteristic signals on the FTIR spectra, the success of the synthesis was evaluated. Lastly, the overall density and porosity using the GeoPyc density analyser, and degradation properties using thermogravimetric analysis (TGA) were determined.
Keywords:polyHIPE, tissue engineering, poly(ethylene glycol), metabolic activity of cells, PEGDMA/PEGDA


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