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Title:Bioactive functional nanolayers of chitosan-lysine surfactant with single- and mixed-protein-repellent and antibiofilm properties for medical implants
Authors:ID Ajdnik, Urban (Author)
ID Fras Zemljič, Lidija (Author)
ID Plohl, Olivija (Author)
ID Pérez, Lourdes (Author)
ID Trček, Janja (Author)
ID Bračič, Matej (Author)
ID Mohan, Tamilselvan (Author)
Files:.pdf ajdnik-et-al-2021-bioactive-functional-nanolayers-of-chitosan-lysine-surfactant-with-single-and-mixed-protein-repellent.pdf (4,24 MB)
MD5: AB33C47B2993FC98239EDF17508702AC
 
URL https://pubs.acs.org/doi/10.1021/acsami.1c01993#
 
Language:English
Work type:Unknown
Typology:1.01 - Original Scientific Article
Organization:FS - Faculty of Mechanical Engineering
Abstract:Medical implant-associated infections resulting from biofilm formation triggered by unspecific protein adsorption arethe prevailing cause of implant failure. However, implant surfaces rendered with multifunctional bioactive nanocoatings offer apromising alternative to prevent the initial attachment of bacteria and effectively interrupt biofilm formation. The need to researchand develop novel and stable bioactive nanocoatings for medical implants and a comprehensive understanding of their properties incontact with the complex biological environment are crucial. In this study, we developed an aqueous stable and crosslinker-freepolyelectrolyte−surfactant complex (PESC) composed of a renewable cationic polysaccharide, chitosan, a lysine-based anionicsurfactant (77KS), and an amphoteric antibiotic, amoxicillin, which is widely used to treat a number of infections caused by bacteria.We successfully introduced the PESC as bioactive functional nanolayers on the“model”and“real”polydimethylsiloxane (PDMS)surfaces under dynamic and ambient conditions. Besides their high stability and improved wettability, these uniformly depositednanolayers (thickness: 44−61 nm) with mixed charges exhibited strong repulsion toward three model blood proteins (serumalbumin,fibrinogen, andγ-globulin) and their competitive interactions in the mixture in real-time, as demonstrated using a quartzcrystal microbalance with dissipation (QCM-D). The functional nanolayers with a maximum negative zeta potential (ζ:−19 to−30mV at pH 7.4), water content (1628−1810 ng cm−2), and hydration (low viscosity and elastic shear modulus) correlated with themass, conformation, and interaction nature of proteins. In vitro antimicrobial activity testing under dynamic conditions showed thatthe charged nanolayers actively inhibited the growth of both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcusaureus) bacteria compared to unmodified PDMS. Given the ease of fabrication of multifunctional and charged biobased coatingswith simultaneous protein-repellent and antimicrobial activities, the limitations of individual approaches could be overcome leadingto a better and advanced design of various medical devices (e.g., catheters, prosthetics, and stents).
Keywords:silicone implants, protein-repellent, antimicrobial, chitosan, lysine, bioactive coatings, adsorption, QCM-D
Publication status:Published
Publication version:Version of Record
Submitted for review:29.01.2021
Article acceptance date:04.05.2021
Publication date:17.05.2021
Publisher:ACS Publications
Year of publishing:2021
Number of pages:str. 23352–23368
Numbering:Vol. 13, iss. 20
PID:20.500.12556/DKUM-88322 New window
UDC:677.017:579
ISSN on article:1944-8244
COBISS.SI-ID:63734787 New window
DOI:10.1021/acsami.1c01993 New window
Publication date in DKUM:15.04.2024
Views:266
Downloads:17
Metadata:XML DC-XML DC-RDF
Categories:Misc.
:
AJDNIK, Urban, FRAS ZEMLJIČ, Lidija, PLOHL, Olivija, PÉREZ, Lourdes, TRČEK, Janja, BRAČIČ, Matej and MOHAN, Tamilselvan, 2021, Bioactive functional nanolayers of chitosan-lysine surfactant with single- and mixed-protein-repellent and antibiofilm properties for medical implants. ACS applied materials & interfaces [online]. 2021. Vol. 13, no. 20. [Accessed 13 April 2025]. DOI 10.1021/acsami.1c01993. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=88322
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Record is a part of a journal

Title:ACS applied materials & interfaces
Shortened title:ACS appl. mater. interfaces
Publisher:American Chemical Society
ISSN:1944-8244
COBISS.SI-ID:14731286 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P2-0118-2015
Name:Tekstilna kemija
Funder:ARIS - Slovenian Research and Innovation Agency
Project number:J4-1764-2019
Name:Razvoj multifunkcionalnih polisaharidnih kompozitnih nanodelcev za razkislinjenje, izboljšanje trdnosti in preprečevanje mikrobiološkega napada zgodovinskih artefaktov na osnovi celuloze

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Abstract:
Keywords:silikonski vsadki, biomedicinski materiali, protimikrobnost, hitozan, bioaktivni premazi, adsorpcija


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