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Title:Vpliv prehrane in starosti goveda na kolonizacije s Clostridioides difficile : magistrsko delo
Authors:ID Erker, Urška (Author)
ID Brus, Maksimiljan (Mentor) More about this mentor... New window
ID Rupnik, Maja (Comentor)
ID Hohler, Anton (Comentor)
Files:.pdf MAG_Erker_Urska_2022.pdf (18,94 MB)
MD5: 0AEC728878D6A830BB47CE29BA847978
PID: 20.500.12556/dkum/15cc6c9c-b7a6-499f-a185-5be9d458fb49
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FKBV - Faculty of Agriculture and Life Sciences
Abstract:Clostridioides difficile je anaerobna, sporogena bakterija, ki ob porušeni normalni črevesni flori proizvaja toksine in tako povzroči širok spekter črevesnih okužb pri ljudeh in živalih. V okviru magistrskega dela smo želeli raziskati vpliv spremenjene celotne krmne mešanice za govedo (TMR) na kolonizacije s C. difficile v fekalni mikrobioti 30-ih krav molznic glede na zaporedno laktacijo (1.‒6.) in fazo laktacije (I., II., III.). Prav tako smo v raziskavo vključili tudi 32 telet iz 6-ih starostnih skupin, da bi proučili, ali obstaja vpliv starosti in prehrane na kolonizacije s C. difficile v telečjem fecesu. Skupno smo 92 vzorcev inkubirali v bogatitvenem gojišču BHI z dodanim L-cisteinom, kvasnim ekstraktom in tauroholatom (BHIST) in kulturo po alkoholnem šoku inkubirali na selektivnem kromogenem gojišču. Analizo smo izvedli v laboratoriju Katedre za mikrobiologijo na Medicinski fakulteti Univerze v Mariboru. Noben fekalni vzorec ni bil pozitiven na bakterijo C. difficile. Na podlagi rezultatov sklepamo, da na kmetiji, zajeti v raziskavo, bakterija C. difficile v fecesu krav molznic in telet ni prisotna.
Keywords:krave molznice, TMR obrok, teleta, Clostridioides difficile, fekalni vzorci
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[U. Erker]
Year of publishing:2022
Number of pages:X, 84 f., [1] f. pril.
PID:20.500.12556/DKUM-82116 New window
UDC:636.2.084:591.13:579.852(043)
COBISS.SI-ID:117244931 New window
Publication date in DKUM:03.08.2022
Views:801
Downloads:138
Metadata:XML DC-XML DC-RDF
Categories:FKBV
:
ERKER, Urška, 2022, Vpliv prehrane in starosti goveda na kolonizacije s Clostridioides difficile : magistrsko delo [online]. Master’s thesis. Maribor : U. Erker. [Accessed 10 April 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=82116
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:14.07.2022

Secondary language

Language:English
Title:Influence of Diet and Cattle Age on Colonizations with Clostridioides difficile
Abstract:Clostridioides difficile is an anaerobic, spore-forming bacterium that produces toxins when the normal intestinal flora is disrupted, causing a wide range of intestinal infections in humans and animals. This work aimed to investigate the influence of altered total mixed rations (TMR) on C. difficile colonies in the faecal microbiota of 30 dairy cows, according to successive lactations (1st‒6th) and lactation phases (I., II., III.). We also included 32 calves from 6 age groups in the study to determine if there was an effect of age and diet on colonizations with C. difficile in calf faeces. A total of 92 samples were incubated in an enrichment BHI broth with L-cysteine, yeast extract and taurocholate added (BHIST), subjected to alcohol shock and isolated on chromogenic plates. The analysis was performed in the laboratory of the Department of Microbiology at the Medical Faculty of the University of Maribor. Overall, no sample was positive for C. difficile. Our results indicate that C. difficile is not present in the faeces of dairy cows and calves on the farm included in the study.
Keywords:dairy cows, TMR feed ration, calves, Clostridioides difficile, faecal samples


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