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Title:Vpliv sestave gojišča na odpornost ocetnokislinskih bakterij proti antibiotikom : magistrsko delo
Authors:ID Cepec, Eva (Author)
ID Trček, Janja (Mentor) More about this mentor... New window
Files:.pdf MAG_Cepec_Eva_2021.pdf (1,32 MB)
MD5: 7A3EF865E4545F31CADF1E72DB2B790D
PID: 20.500.12556/dkum/e7315a9f-a992-4268-8708-64852d6766ca
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:Zaradi vse večje ozaveščenosti potrošnikov o pomenu uživanja naravnih živil se povečuje tudi povpraševanje po jabolčnem nefiltriranem kisu, tako imenovanem motnem jabolčnem kisu. Motni jabočlni kis poleg ostalih sestavin vsebuje tudi ocetnokislinske bakterije. Povečan trend uporabe takšnega kisa pa pred proizvajalca in potrošnika postavlja vprašanje o potencialni odpornosti ocetnokislinskih bakterij proti antibiotikom. V tem delu smo zato preiskali odpornost različnih vrst in rodov ocetnokislinskih bakterij proti klinično pomembnim antibiotikom (ampicilinu, kloramfenikolu, ciprofloksacinu, trimetoprimu, eritromicinu in gentamicinu) na treh rastnih gojiščih (RAE, MA in GY). Odpornost smo analizirali z disk-difuzijsko metodo glede na prisotnost/odsotnost cone inhibicije okoli diskov z antibiotiki. Preiskali smo 12 sevov iz rodu Acetobacter in 22 predstavnikov iz rodu Komagateibacter. Delež odpornih sevov proti posameznemu antibiotiku se je med rastnimi gojišči razlikoval, na vseh gojiščih pa so bili preiskani sevi v visokem deležu odporni proti trimetoprimu (97,1 %), eritromicinu (73,5 %), ciprofloksacinu (50,0 %) in kloramfenikolu (32,4 %). Rezistenco proti štirim antibiotikom smo ugotovili pri kar 17,6 % sevov, 29,4 % testiranih sevov pa je bilo odpornih proti trem antibiotikom. Ker ocetnokislinske bakterije ali pa njihova DNA po zaužitju kisa, kefirja in drugih živilskih proizvodov, pri katerih sodelujejo ocetnokislinske bakterije, lahko pride v kontakt z mikrobioto prebavil človeka, bi bilo v nadaljevanju potrebno preučiti možnost horizontalnega prenosa genov za odpornost proti antibiotikom iz ocetnokislinskih bakterij v bakterije, ki sestavljajo mikrobioto prebavil človeka.
Keywords:ocetnokislinske bakterije, odpornost proti antibiotikom
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[E. Cepec]
Year of publishing:2021
Number of pages:VIII, 57 f.
PID:20.500.12556/DKUM-80937 New window
UDC:579:575(043.2)
COBISS.SI-ID:90076931 New window
Publication date in DKUM:20.12.2021
Views:1572
Downloads:122
Metadata:XML DC-XML DC-RDF
Categories:FNM
:
CEPEC, Eva, 2021, Vpliv sestave gojišča na odpornost ocetnokislinskih bakterij proti antibiotikom : magistrsko delo [online]. Master’s thesis. Maribor : E. Cepec. [Accessed 15 April 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=80937
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:23.11.2021

Secondary language

Language:English
Title:The influence of Growth Medium Composition on Antibiotic Resistance of Acetic Acid Bacteria
Abstract:Due to the growing awareness of consumers about the importance of consuming natural foods, the demand for apple cider vinegar that is not filtered at the end of the technological process is increasing. The unfiltered apple cider vinegar contains in addition to other ingredients, also acetic acid bacteria. Increasing trends of using this type of vinegar raises a question of potential resistance of the acetic acid bacteria to antibiotics. In this work, we therefore investigated the resistance of different species and genera of acetic acid bacteria against clinically relevant antibiotics (ampicillin, chloramphenicol, ciprofloxacin, trimethoprim, erythromycin, and gentamicin) on three growth media (RAE, MA and GY). We analyzed resistance by the disk-diffusion method with respect to the presence/absence of inhibition zones around the discs with antibiotics. We investigated 12 strains of the genus Acetobacter and 22 representatives of the genus Komagateibacter. The percentages of resistant strains against antibiotics varied among growth media. However, on all three media high percentages of strains were resistant against trimethoprim (97.1%), erythromycin (73.5%), ciprofloxacin (50.0%) and chloramphenicol (32.5%). Resistance against four antibiotics was detected in 17.6% of strains and 29.4% of tested strains were resistant to three antibiotics. Because the acetic acid bacteria or their DNA may come into contact with the microbiota of the human gastrointestinal tract after ingestion of vinegar, kefir and other foods produced by acetic acid bacteria, it will be necessary to analyze in the future a possibility of horizontal transfer of antibiotic resistance gene determinants from acetic acid bacteria to human bacterial gastrointestinal microbiota.
Keywords:acetic acid bacteria, antibiotic resistance


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