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Title:
Izolacija kanabinoidov iz industrijske konoplje : magistrsko delo
Authors:
ID
Horvat, Domen
(Author)
ID
Kotnik, Petra
(Mentor)
More about this mentor...
ID
Knez Marevci, Maša
(Comentor)
Files:
MAG_Horvat_Domen_2020.pdf
(2,25 MB)
MD5: 93C3EC30C9AEF6D113DF1AB95BFB634B
PID:
20.500.12556/dkum/eda08e8d-305a-4dd5-bc66-64f01fda41d4
Language:
Slovenian
Work type:
Master's thesis/paper
Typology:
2.09 - Master's Thesis
Organization:
FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:
Magistrsko delo prikazuje študijo različnih tehnik izolacije in separacije kanabinoidov iz industrijske konoplje, njihovo dekarboksilacijo ter antioksidativne lastnosti. Kanabinoide iz industrijske konoplje smo izolirali z različnimi ekstrakcijskimi metodami in primerjali rezultate vsebnosti posameznih kanabinoidov, ki so bile določene s pomočjo tekočinske kromatografije (LC-MS). Pričakovano smo večjo vsebnost kislinskih kanabinoidov zabeležili v ekstraktih, ki so bili pridobljeni pri nižjih temperaturah (maceracija), medtem ko so pri višjih temperaturah bili ekstrakti podvrženi procesu dekarboksilacije ter posledično vsebovali več nekislinskih kanabinoidov. Posamezne kanabinoide iz pripravljenih ekstraktov smo ločevali s tankoplastno kromatografijo sklopljeno z masnim spektrometrom (TLC-MS). Preverili smo vpliv pomembnih kromatografskih parametrov (relativna vlažnost, temperatura, mobilna faza) na učinkovitost ločbe. Ugotovili smo, da je ločba posameznih kanabinoidov izjemno zahtevna. Zanimal nas je tudi vpliv temperature, časa in oblike, v kateri se kanabinoidi nahajajo, na proces dekarboksilacije kislinskih kanabinoidov. Opazili smo, da hitrost dekarboksilacije s temperaturo in časom močno naraste. Kanabinoidi v ekstraktih so za popolno dekarboksilacijo kislinskih kanabinoidov potrebovali manj časa kot kanabinoidi v rastlinskem materialu. Antioksidativne lastnosti posameznih ekstraktov in kanabinoidov smo določili s pomočjo DPPH in β-karotenske metode, ter jih primerjali z dvema pogosto uporabljenima antioksidantoma - askorbinsko kislino ter BHT. Izsledki obeh metod so potrdili izjemen antioksidativni potencial kanabinoidov v ekstraktih, ki so pokazali primerljive rezultate kot askorbinska kislina. Posamezni standardi kanabinoidov so antioksidativno manj učinkoviti, kar nakazuje na pomembnost sinergističnega učinka kanabinoidov.
Keywords:
kanabinoidi
,
LC-MS
,
TLC-MS
,
dekarboksilacija
,
antioksidativnost
Place of publishing:
Maribor
Place of performance:
Maribor
Publisher:
[D. Horvat]
Year of publishing:
2020
Number of pages:
VIII, 50 str.
PID:
20.500.12556/DKUM-77872
UDC:
66.061.34:633.522(043.2)
COBISS.SI-ID:
32126211
NUK URN:
URN:SI:UM:DK:7ZIWMLQH
Publication date in DKUM:
08.10.2020
Views:
1146
Downloads:
246
Metadata:
Categories:
KTFMB - FKKT
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:
HORVAT, Domen, 2020,
Izolacija kanabinoidov iz industrijske konoplje : magistrsko delo
[online]. Master’s thesis. Maribor : D. Horvat. [Accessed 24 April 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=77872
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Licences
License:
CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:
http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:
The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:
18.09.2020
Secondary language
Language:
English
Title:
Isolation of cannabinoids from industrial hemp
Abstract:
The master's thesis presents a study of various techniques for the isolation and separation of cannabinoids from industrial hemp, their decarboxylation and antioxidant properties. Cannabinoids from industrial hemp were isolated using various extraction methods.The amount of individual cannabinoids was determined by liquid chromatography (LC-MS). As expected, a higher content of acidic cannabinoids was reported in extracts obtained at lower temperatures (maceration), while at higher temperatures the extracts were subjected to a decarboxylation process and consequently contained more non-acidic cannabinoids. Individual cannabinoids from the prepared extracts were separated with thin layer chromatography coupled with mass spectrometry (TLC-MS). The influence of important chromatographic parameters (relative humidity, temperature, mobile phase) on the separation efficiency has been studied. The separation of individual cannabinoids has proven to be extremely challenging. The influence of temperature, time and the form in which cannabinoids are found during the process of decarboxylation of acid cannabinoids has been investigated. Results clearly show that the rate of decarboxylation increases sharply with temperature and time. The cannabinoids in the extracts took less time to completely undergo decarboxylation than the cannabinoids in the plant material. The antioxidant properties of individual extracts and cannabinoids were determined using DPPH and β-carotene methods, and compared with two commonly used antioxidants - ascorbic acid and BHT. The results of both methods confirmed the exceptional antioxidant potential of cannabinoids in the extracts, which showed comparable results as ascorbic acid. Individual standards of cannabinoids have shown a lower antioxidant activity, suggesting the importance of the synergistic effect of cannabinoids.
Keywords:
cannabinoids
,
LC-MS
,
TLC-MS
,
decarboxylation
,
antioxidative properties
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