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Title:Skupne genske značilnosti bolnikov z astmo in atopijskim dermatitisom : diplomsko delo visokošolskega strokovnega študijskega programa I. stopnje
Authors:ID Jeraj, Amadeja (Author)
ID Potočnik, Uroš (Mentor) More about this mentor... New window
ID Repnik, Katja (Comentor)
ID Skerbinjek-Kavalar, Maja (Comentor)
Files:.pdf VS_Jeraj_Amadeja_2020.pdf (1,67 MB)
MD5: AE3F27ED0FF1304254693CDDEF695ED1
PID: 20.500.12556/dkum/e7aab0b9-d91a-44b4-91e1-23331cfbb4c2
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Astma in atopijski dermatitis sta kompleksni alergijski bolezni, katerih prisotnost v današnjem razvitem svetu narašča. K razvoju obeh bolezni pripomore več različnih genov, bivalno okolje in način življenja posameznika. Namen raziskave je bil poiskati podobnosti in razlike v genetski arhitekturi astme in atopijskega dermatitisa. Na osnovi objavljenih študij smo izbrali tiste gene, ki so bili povezani z nastankom obeh boleznih ter analizirali genetske polimorfizme v izbranih genih pri slovenskih otrocih z astmo in/ali atopijskim dermatitisom. V našo študijo smo vključili 219 bolnikov in 97 zdravih posameznikov kot kontrolno skupino. Iz brisov ustne sluznice in krvnih limfocitov bolnikov ter zdravih posameznikov smo izolirali DNA molekule, na katerih smo genotipizirali izbrane polimorfizme genov STAT5B (rs9900213), TSLP (rs1898671) in STAT6 (rs324015). Genotipizacijo smo izvedli z metodoma qPCR-HRM in PCR-RFLP. Rezultate smo statistično analizirali s programom SPSS, kjer smo primerjali genotipske in alelne frekvence bolnikov in kontrol ter iskali povezavo med polimorfizmi in boleznima. V raziskovalni nalogi smo potrdili vpliv gena TSLP na razvoj astme. Sočasno smo ugotovili, nekoliko povišano frekvenco alela T polimorfizma rs9900213 v genu STAT5B pri astmatikih in bolnikih z atopijskim dermatitisom in pridruženo astmo, kar bi lahko povečalo tveganje za razvoj astme. Za boljše razumevanje povezave astme in atopijskega dermatitisa bi bile potrebne nadaljnje raziskave na večjem številu vzorcev. S tem bi dobili realnejšo sliko povezav med atopijskim dermatitisom in astmo, kar bi prispevalo k uspešnejšemu zdravljenju. Pridobljeni podatki lahko v prihodnosti prispevajo k razumevanju povezav med astmo in atopijskim dermatitisom.
Keywords:atopijski dermatitis, astma, asociacijska študija, polimorfizem posameznega nukleotida (SNP), kandidatni geni
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[A. Jeraj]
Year of publishing:2020
Number of pages:X, 33 f.
PID:20.500.12556/DKUM-76770 New window
UDC:575.1:616-056.7(043.2)
COBISS.SI-ID:24525059 New window
NUK URN:URN:SI:UM:DK:ZSNBRBX6
Publication date in DKUM:30.07.2020
Views:1433
Downloads:131
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
:
JERAJ, Amadeja, 2020, Skupne genske značilnosti bolnikov z astmo in atopijskim dermatitisom : diplomsko delo visokošolskega strokovnega študijskega programa I. stopnje [online]. Bachelor’s thesis. Maribor : A. Jeraj. [Accessed 18 April 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=76770
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:03.07.2020

Secondary language

Language:English
Title:Shared genetic characteristics of asthma and atopic dermatitis
Abstract:Asthma and atopic dermatitis are a complex allergic disease, the presence of which is increasing in today's developed world. To the development of this disease contributes several different genes, living environment and lifestyle of the individual. The purpose of the study was to find similarities in differences in the genetic architecture of asthma in atopic dermatitis. Based on the published study, we selected the appropriate genes, which were associated with the occurrence among both diseases, and analyzed genetic polymorphisms in selected genes in Slovenian children with asthma and / or atopic dermatitis. We included 219 patients and 97 healthy individuals as control groups in our study. DNA molecules were isolated from swabs of the oral mucosa and blood lymphocytes of patients and healthy individuals, on which selected polymorphisms of the STAT5B (rs9900213), TSLP (rs1898671) and STAT6 (rs324015) genes were genotyped. Genotyping was performed by qPCR-HRM and PCR-RFLP methods. The results were statistically analyzed with the SPSS program, where we compared the genotypic and allelic frequencies of patients and controls and looked for a link between polymorphisms and diseases. In a research paper, we confirmed the influence of the TSLP gene on the development of asthma. At the same time, we found a slightly increased frequency of the T allele of the rs9900213 polymorphism in the STAT5B gene in asthmatics and patients with atopic dermatitis and associated asthma, which could increase the risk of developing asthma.To better understand the link between asthma and atopic dermatitis, further research on a larger number of samples would be needed. This would give a more realistic picture of the links between atopic dermatitis and asthma, which would lead to more successful treatment. The data obtained may contribute in the future to understanding the link between asthma and atopic dermatitis.
Keywords:atopic dermatitis, asthma, association studies, single nucleotide polymorphism (SNP), candidate genes


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