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Title:In vitro tissue culture initiation from potted and garden Hydrangea macrophylla explants
Authors:ID Šiško, Metka (Author)
Files:.pdf Agricultura_2016_Sisko_In_vitro_tissue_culture_initiation_from_potted_and_garden_Hydrangea_macrophylla_explants.pdf (271,02 KB)
MD5: 5725D3F2B8026465544872A6B219A7C3
PID: 20.500.12556/dkum/78bbc32c-2ff7-4d00-a55e-fee54243bd70
 
URL http://www.degruyter.com/view/j/agricultura.2016.13.issue-1-2/agricultura-2017-0008/agricultura-2017-0008.xml
 
Language:English
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:FKBV - Faculty of Agriculture and Life Sciences
Abstract:The effect of sterilisation treatments on the initiation of the culture of three Hydrangea macrophylla genotypes in vitro was studied. The results indicated difference among different disinfection treatment; the consequence of treatments with dichloroisocyanuric acid, in combination with silver nitrate, was a significantly higher survival rate comparing to treatments based on NaOCl. Success of sterilisation was significantly influenced by studied genotypes. Two potted hydrangea genotypes (G2 and G3) showed higher survival rate (45.00 %, 55.00 %, respectively) comparing to the genotype 1 (originated from a local garden), which exhibited the highest percentage of contamination rate (58.75 %). Culturing the explants on a McCown woody plant medium supplemented with BAP at 2 mg/L and NAA at 0.005 mg/L produced the highest number of shoots with multiplication rate of 1.31 in 5 weeks. The highest rooting percentage (100.00 %) was obtained with the genotype 2 on a McCown woody plant medium supplemented with 0.5 mg/L IBA.
Keywords:micropropagation, tissue culture, hydrangea, Hydrangea macrophylla, plant disinfection
Publication status:Published
Publication version:Version of Record
Year of publishing:2016
Number of pages:str. 65-69
Numbering:Letn. 13, št. 1/2
PID:20.500.12556/DKUM-68965 New window
ISSN:1580-8432
UDC:631.526:582.785
ISSN on article:1580-8432
COBISS.SI-ID:4309804 New window
DOI:10.1515/agricultura-2017-0008 New window
NUK URN:URN:SI:UM:DK:Q4BZEHYQ
Publication date in DKUM:14.11.2017
Views:1947
Downloads:421
Metadata:XML DC-XML DC-RDF
Categories:Misc.
:
ŠIŠKO, Metka, 2016, In vitro tissue culture initiation from potted and garden Hydrangea macrophylla explants. Agricultura [online]. 2016. Vol. 13, no. 1/2, p. 65–69. [Accessed 7 April 2025]. DOI 10.1515/agricultura-2017-0008. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=68965
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Record is a part of a journal

Title:Agricultura
Publisher:Univerza v Mariboru, Fakulteta za kmetijstvo, Univerza v Mariboru, Fakulteta za kmetijstvo, Univerzitetna založba Univerze v Mariboru
ISSN:1580-8432
COBISS.SI-ID:116855808 New window

Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:14.11.2017

Secondary language

Language:Slovenian
Title:Iniciacija rastlinske tkivne kulture lončne in vrtne hortenzije (Hydrangea macrophylla) v in vitro razmerah
Abstract:Proučevali smo vpliv različnih načinov sterilizacije na uspešno vzpostavitev tkivne kulture treh genotipov hortenzije (Hydrangea macrophylla). Ugotovili smo, da obstajajo razlike med različnimi načini sterilizacije. Sterilizacija z dikloroizocianurno kislino v kombinaciji s srebrovim nitratom je bila uspešnejša od sterilizacije z uporabo NaOCl. Na uspešnost sterilizacije je vplival tudi genotip. Lončne hortenzije (genotipa G2 in G3) so kazale večjo sposobnost sterilizacije (pri genotipu G2 smo tako dobili 45.00 %, pri G3 pa 55.00 % uspešno steriliziranih poganjkov) v primerjavi z vrtno hortenzijo, kjer je bilo največ okuženih poganjkov (58.75 %). Za namnoževanje poganjkov se je kot najboljše izkazalo gojišče McCown z dodatkom 2 mg/L BAP in 0.005 NAA, na katerem je bil faktor namnoževanja 1,31 v petih tednih. Najbolje je koreninil genotip 2 na McCown osnovnem gojišču z dodano 0,5 mg/L IBA in sicer je na tem gojišču koreninilo 100 % poganjkov.
Keywords:mikropropagacija, tkivne kulture, hortenzija, Hydrangea macrophylla, sterilizacija rastlin


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