| | SLO | ENG | Cookies and privacy

Bigger font | Smaller font
First page > Show document

Show document Help

Title:Typing Clostridium difficile strains based on tandem repeat sequences
Authors:ID Henning Zaiß, N (Author)
ID Rupnik, Maja (Author)
ID Kuijper, Ed J (Author)
ID Harmanus, Celine (Author)
ID Michielsen, Dolf (Author)
ID Janssens, Koen (Author)
ID Nübel, Ulrich (Author)
Files:.pdf BMC_Microbiology_2009_ZaiSS_et_al._Typing_Clostridium_difficile_strains_based_on_tandem_repeat_sequences.pdf (792,24 KB)
MD5: 7935102399729F22747BA7836244BE54
 
URL http://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-9-6
 
Language:English
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Abstract:Background: Genotyping of epidemic Clostridium difficile strains is necessary to track their emergence and spread. Portability of genotyping data is desirable to facilitate inter-laboratory comparisons and epidemiological studies. Results: This report presents results from a systematic screen for variation in repetitive DNA in the genome of C. difficile. We describe two tandem repeat loci, designated 'TR6' and 'TR10', which display extensive sequence variation that may be useful for sequence-based strain typing. Based on an investigation of 154 C. difficile isolates comprising 75 ribotypes, tandem repeat sequencing demonstrated excellent concordance with widely used PCR ribotyping and equal discriminatory power. Moreover, tandem repeat sequences enabled the reconstruction of the isolates' largely clonal population structure and evolutionary history. Conclusion: We conclude that sequence analysis of the two repetitive loci introduced here may be highly useful for routine typing of C. difficile. Tandem repeat sequence typing resolves phylogenetic diversity to a level equivalent to PCR ribotypes. DNA sequences may be stored in databases accessible over the internet, obviating the need for the exchange of reference strains.
Keywords:Clostridium difficile, microbiology, strain typing
Publication status:Published
Publication version:Version of Record
Year of publishing:2009
Number of pages:str. 1-11
Numbering:Letn. 9
PID:20.500.12556/DKUM-66508 New window
ISSN:1471-2180
UDC:579.6
ISSN on article:1471-2180
COBISS.SI-ID:652063 New window
DOI:10.1186/1471-2180-9-6 New window
NUK URN:URN:SI:UM:DK:DFSXNGHG
Publication date in DKUM:29.06.2017
Views:1585
Downloads:403
Metadata:XML DC-XML DC-RDF
Categories:Misc.
:
HENNING ZAISS, N, RUPNIK, Maja, KUIJPER, Ed J, HARMANUS, Celine, MICHIELSEN, Dolf, JANSSENS, Koen and NÜBEL, Ulrich, 2009, Typing Clostridium difficile strains based on tandem repeat sequences. BMC Microbiology [online]. 2009. Vol. 9, p. 1–11. [Accessed 18 April 2025]. DOI 10.1186/1471-2180-9-6. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=66508
Copy citation
  
Average score:
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
(0 votes)
Your score:Voting is allowed only for logged in users.
Share:Bookmark and Share


Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Record is a part of a journal

Title:BMC Microbiology
Shortened title:BMC Microbiol.
Publisher:BioMed Central
ISSN:1471-2180
COBISS.SI-ID:2441492 New window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:29.06.2017

Secondary language

Language:Slovenian
Keywords:Clostridium difficile, mikrobiologija, tipizacija sevov


Comments

Leave comment

You must log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back
Logos of partners University of Maribor University of Ljubljana University of Primorska University of Nova Gorica