| | SLO | ENG | Cookies and privacy

Bigger font | Smaller font
First page > Show document

Show document Help

Title:A co-culture model of the developing small intestine offers new insight in the early immunomodulation of enterocytes and macrophages by Lactobacillus spp. through STAT1 and NF-kB p65 translocation
Authors:ID Trapečar, Martin (Author)
ID Goropevšek, Aleš (Author)
ID Gorenjak, Mario (Author)
ID Gradišnik, Lidija (Author)
ID Rupnik, Marjan (Author)
Files:.pdf PLoS_ONE_2014_Trapecar_et_al._A_Co-Culture_Model_of_the_Developing_Small_Intestine_Offers_New_Insight_in_the_Early_Immunomodulation_of_E.PDF (2,86 MB)
MD5: F53EB2983D397E8F292C7D208E876F5B
 
URL http://dx.plos.org/10.1371/journal.pone.0086297
 
Language:English
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Abstract:The early establishment of a complete microbiome has been shown to play an integral part in the development and maintenance of an intact intestine and its immune system, although much remains unknown about the specific mechanisms of immune modulation in newborns. In our study we show in a co-culture model of the undeveloped small intestine that members of Lactobacillus spp. influence STAT1 and NF-kB p65 nuclear translocation in both intestinal epithelial cells as well as underlying macrophages. Moreover, by using imaging flow cytometry we were able to monitor each individual cell and create a framework of the percentage of cells in which translocation occurred in challenged versus control cell populations. We also observed a significant difference in baseline translocation in intestinal cells when cultured alone versus those in a co-culture model, underpinning the importance of 3D models over monolayer set-ups in epithelial in vitro research. In conclusion, our work offers new insights into the potential routes by which the commensal microbiome primes the early immune system to fight pathogens, and shows how strain-specific these mechanisms really are.
Keywords:microbiome, Lactobacillus, immune system, pathogens
Publication status:Published
Publication version:Version of Record
Year of publishing:2014
Number of pages:str. 1-8
Numbering:Letn. 9, št. 1
PID:20.500.12556/DKUM-66266 New window
ISSN:1932-6203
UDC:579.864
ISSN on article:1932-6203
COBISS.SI-ID:512370232 New window
DOI:10.1371/journal.pone.0086297 New window
NUK URN:URN:SI:UM:DK:AZJFRQRO
Publication date in DKUM:19.06.2017
Views:1485
Downloads:372
Metadata:XML DC-XML DC-RDF
Categories:Misc.
:
TRAPEČAR, Martin, GOROPEVŠEK, Aleš, GORENJAK, Mario, GRADIŠNIK, Lidija and RUPNIK, Marjan, 2014, A co-culture model of the developing small intestine offers new insight in the early immunomodulation of enterocytes and macrophages by Lactobacillus spp. through STAT1 and NF-kB p65 translocation. PloS one [online]. 2014. Vol. 9, no. 1, p. 1–8. [Accessed 9 April 2025]. DOI 10.1371/journal.pone.0086297. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=66266
Copy citation
  
Average score:
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
(0 votes)
Your score:Voting is allowed only for logged in users.
Share:Bookmark and Share


Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Record is a part of a journal

Title:PloS one
Publisher:Public Library of Science
ISSN:1932-6203
COBISS.SI-ID:2005896 New window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:19.06.2017

Secondary language

Language:Slovenian
Keywords:mikrobiom, Lactobacillus, imunski sistem, patogeni


Comments

Leave comment

You must log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back
Logos of partners University of Maribor University of Ljubljana University of Primorska University of Nova Gorica