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Title:Effect of peptides' binding on the antimicrobial activity and biocompatibility of ‎protein-based substrates ‎
Authors:ID Kaisersberger Vincek, Maja (Author)
ID Kokol, Vanja (Mentor) More about this mentor... New window
Files:.pdf DOK_Kaisersberger_Vincek_Maja_2017.pdf (3,98 MB)
MD5: 33BA98E9E6CA1CA5567FDCFD1B1A8ABE
 
Language:English
Work type:Dissertation
Typology:2.08 - Doctoral Dissertation
Organization:FS - Faculty of Mechanical Engineering
Abstract:This work reveals the effect of coupling approach (chemical by using carbodiimide chemistry ‎and grafting-to vs. grafting-from synthesis routes, and enzymatic by using transglutaminase) ‎of a hydrophilic ε-poly-L-lysine (εPL) and an amphiphilic oligo-acyl-lysyl (OAK) derivative (K-7α12-OH) to wool fibers and gelatine (GEL) macromolecules, respectively, and substrates ‎antibacterial activity against Gram-negative E. coli and Gram-positive S. aureus bacteria ‎after 1–24 h of exposure, as well as their cytotoxicity. Different spectroscopic (ultraviolet-‎visible, infrared, fluorescence and electron paramagnetic resonance) and separation ‎techniques (size-exclusion chromatography and capillary zone electrophoresis) as well as ‎zeta potential and potentiometric titration analysis, were performed to confirm the covalent ‎coupling of εPL/OAK, and to determine the amount and orientation of its immobilisation.‎ The highest and kinetically the fastest level of bacterial reduction was achieved with ‎wool/GEL functionalised with εPL/OAK by chemical grafting-to approach. This effect ‎correlated with both the highest grafting yield and conformationally the highly-flexible ‎(brush-like) orientation linkage of εPL/OAK, implicating on the highest amount of accessible ‎amino groups interacting with bacterial membrane. However, OAK`s amphipathic structure, ‎the cationic charge and the hydrophobic moieties, resulted to relatively high reduction of S. ‎aureus for grafting-from and the enzymatic coupling approaches using OAK-functionalised ‎GEL. ‎ The εPL/OAK-functionalised GEL did not induce toxicity in human osteoblast cells, even at ‎‎~25-fold higher concentration than bacterial minimum inhibitory (MIC) concentration of ‎εPL/OAK, supporting their potential usage in biomedical applications.‎ It was also shown that non-ionic surfactant adsorbs strongly onto the wool surface during ‎the process of washing, thereby blocking the functional sites of immobilized εPL and ‎decreases its antibacterial efficiency. ‎ ‎
Keywords:wool, gelatine, antimicrobial peptides, ε-poly-L-lysine, oligo-acyl-lysyl, grafting chemistry, ‎grafting approach, peptide orientation, antibacterial activity, cytotoxicity‎
Place of publishing:Maribor
Publisher:[M. Kaisersberger Vincek]
Year of publishing:2017
PID:20.500.12556/DKUM-65459 New window
UDC:677.31.017.86:604.4(043.3)
COBISS.SI-ID:20730902 New window
NUK URN:URN:SI:UM:DK:H9R0AYPC
Publication date in DKUM:17.08.2017
Views:1931
Downloads:179
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FS
:
KAISERSBERGER VINCEK, Maja, 2017, Effect of peptides’ binding on the antimicrobial activity and biocompatibility of ‎protein-based substrates ‎ [online]. Doctoral dissertation. Maribor : M. Kaisersberger Vincek. [Accessed 17 April 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=65459
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Secondary language

Language:Slovenian
Title:Vpliv vezave peptidov na protimikrobno aktivnost in biokompatibilnost proteinskih substratov
Abstract:V doktorski nalogi je preučen vpliv načina vezave (kemijski z uporabo karbodiimida in ‎‎»vezavo-na« ‎napram »vezavo-iz« načina sinteze ter encimski z uporabo transglutaminaze, ‎TGaze) hidrofilnega ε-poli-‎L-lizina (εPL) in amfifilnega derivata (K-7α12-OH) oligo-acil-‎lizila (OAK) na volnena vlakna in ‎makromolekule želatine (GEL) ter ovrednotenje ‎inhibitorne aktivnosti substratov na Gram-negativno ‎bakterijo E. coli in Gram-pozitivno S. ‎aureus po 1–24 urni izpostavitvi ter citotoksičnost. Z uporabo ‎različnih spektroskopskih ‎‎(ultravioletna-vidna, infra-rdeča, fluorescenčna, elektronsko paramagnetno ‎resonančna) in ‎separacijskih (visokotlačna tekočinska izločitvena kromatografija, kapilarna ‎conska ‎elektroforeza) analiznih metod, ter zeta potenciala in potenciometrične titracije, je bila ‎potrjena ‎kovalentna vezava εPL/OAK na substrata ter določena kvantiteta in orientacija ‎njihove imobilizacije.‎ Najučinkovitejši in najhitrejši učinek redukcije bakterij je bil dosežen z uporabo ‎volne/GEL ‎funkcionalizirane z εPL/OAK po kemijskem in »vezava-na« načinu. Takšen učinek je ‎povezan ‎tako z največjo količino vezave εPL/OAK kot tudi z njihovo konformacijsko najbolj ‎razvejano ‎˝brush-like˝ orientacijo, ki posledično doprinese največjo količino dostopnih ‎amino skupin za interakcije ‎z bakterijsko membrano. Kljub temu sta oba substrata ‎finkcionalizirana s OAK po kemijskem »vezava-iz« ‎in encimskem načinu vezave izkazala ‎relativno visok inhibitorni učinek na bakterijo S. aureus, zaradi ‎svoje amfifilne strukture – ‎t.j. pozitivnega naboja in hidrofobnih ostankov. ‎ εPL/OAK-funkcionalizirana GEL ni sprožila toksičnega učinka pri človeških osteoblastnih ‎celicah niti pri ‎‎~25-krat višji koncentraciji kot je bakterijska minimalna inhibitorna ‎koncentracija (MIC) za εPL/OAK, kar ‎kaže na njihovo potencialno uporabo v biomedicini.‎ Prav tako je bilo pokazano, da se neionsko površinsko-aktivno sredstvo močno adorbira na ‎volneno ‎površino med procesom pranja, in tako blokira funkcionalne skupine ‎imobiliziranega εPL ter posledično ‎zmanjša protibakterijski učinek. ‎
Keywords:volna, želatina, protimikrobni peptidi, -poli-L-lizin, oligo-acil-lizil, način vezave, ‎konformacija, protibakterijsko delovanje, citotoksičnost‎


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