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Title:A novel approach for assuring and following inoculum activity during kefir grains growth studies : application of dynamic pH profiles
Authors:ID Tramšek, Marko (Author)
ID Goršek, Andreja (Author)
Files:.pdf Acta_Chimica_Slovenica_2007_Tramsek,_Gorsek_A_novel_approach_for_assuring_and_following_inoculum_activity_during_kefir_grains_growth_stu.pdf (384,93 KB)
MD5: E6D0AF5DEDE623654A5CED2BC71FD13D
PID: 20.500.12556/dkum/ac746058-bbd5-4b31-9af9-53b6592a9c5a
 
URL http://acta-arhiv.chem-soc.si/54/54-4-693.pdf
 
Language:English
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:The profiles of kefir grains growth curves strongly depend on bioprocess conditions and inoculum viability. Therefore, accurate growth curve studies require the use of optimally active kefir grains as inoculum. Accordingly, the main objective of our study was experimental optimization of classic kefir grains activation procedure and afterwards, comparison among growth curves of differently activated kefir grains. For this purpose some experiments were initially performed in computer-controlled RC1 reactor provided data of dynamic pH profiles during batch propagation of differently activated grains. Experimental data were mathematically analyzed and using the special methodology of minimizing the absolute deviation of pH(t) profiles the minimal time for grains activation was determined. We established that optimal inoculum activity could be ensured only by the kefir grains, which were previously activated at least over eleven successive days. In the second part of research the growth curve of optimally activated kefir grains was constructed using experimental measurements and fitted with Gompertz model. The present results were compared with the results obtained by our previous study, where classically activated grains were used. We established that, inspite of equal daily kefir grain mass increase, considerable difference in growth curves of differently activated kefir grains exist.
Keywords:chemical processing, kefir grains, growth curve, minimal activation time, dynamic pH profiles, Gompertz model
Publication status:Published
Publication version:Version of Record
Year of publishing:2007
Number of pages:str. 693-699
Numbering:Letn. 54, št. 4
PID:20.500.12556/DKUM-26032 New window
ISSN:1318-0207
UDC:66:519.853
ISSN on article:1318-0207
COBISS.SI-ID:11991062 New window
NUK URN:URN:SI:UM:DK:EFOICXH3
Publication date in DKUM:31.05.2012
Views:2134
Downloads:123
Metadata:XML DC-XML DC-RDF
Categories:Misc.
:
TRAMŠEK, Marko and GORŠEK, Andreja, 2007, A novel approach for assuring and following inoculum activity during kefir grains growth studies : application of dynamic pH profiles. Acta Chimica Slovenica [online]. 2007. Vol. 54, no. 4, p. 693–699. [Accessed 7 April 2025]. Retrieved from: https://dk.um.si/IzpisGradiva.php?lang=eng&id=26032
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Record is a part of a journal

Title:Acta Chimica Slovenica
Shortened title:Acta Chim. Slov.
Publisher:Slovensko kemijsko društvo
ISSN:1318-0207
COBISS.SI-ID:14086149 New window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:31.05.2012

Secondary language

Language:Slovenian
Abstract:Profili krivulj rasti kefirnih zrn so v veliki meri odvisni od bioprocesnih pogojev in aktivnosti inokuluma, zato so za njihove natančne študije potrebna optimalno aktivna zrna. Namen naše raziskave je bil v prvi fazi eksperimentalno optimiranje klasičnega postopka aktivacije kefirnih zrn in nadalje primerjava krivulj rasti različno aktiviranih zrn. V ta namen smo v računalniško vodenem reakcijskem kalorimetru RC1 izvedli serijo eksperimentov, pri katerih smo zasledovali spreminjanje pH vrednosti med šaržnim razmnoževanjem različno aktiviranih kefirnih zrn. Eksperimentalne podatke smo matematično analizirali in hkrati s posebno metodologijo minimiziranja absolutnega odstopanja pH(t) profilov določili minimalni čas aktivacije zrn. Dokazali smo, da optimalno aktivnost inokuluma lahko zagotovimo le z zrni, ki so bila predhodno aktivirana najmanj 11 zaporednih dni. V drugem delu raziskave smo na osnovi eksperimentalnih meritev konstruirali krivuljo rasti optimalno aktiviranih zrn in jo prilegali k Gompertz-ovemu modelu. Dobljene rezultate smo primerjali s podatki iz predhodne raziskave, kjer so bila uporabljena klasično aktivirana zrna. Ugotovili smo, da kljub enakemu dnevnemu prirastu obstajajo znatne razlike v krivuljah rasti različno aktiviranih kefirnih zrn.
Keywords:kemijska procesna tehnika, kefirjeva zrna, modeli rasti, minimalni aktivacijski čas, Gompertzov model


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