| Naslov: | Natural Deep Eutectic Solvent-Based Matrix Solid Phase dispersion (MSPD) extraction for determination of bioactive compounds from sandy everlasting (Helichrysum arenarium L.) : a case of stability study |
|---|
| Avtorji: | ID Ivanović, Milena (Avtor)
ID Krajnc, Peter (Avtor)
ID Mlinarič, Aleš (Avtor)
ID Islamčević Razboršek, Maša (Avtor) |
|---|
| Datoteke: |  plants-11-03468.pdf (2,47 MB)
MD5: 23B22B93EDAF0C7196E3419315B3DF37
 https://www.mdpi.com/2223-7747/11/24/3468
|
|---|
| Jezik: | Angleški jezik |
|---|
| Vrsta gradiva: | Članek v reviji |
|---|
| Tipologija: | 1.01 - Izvirni znanstveni članek |
|---|
| Organizacija: | FKKT - Fakulteta za kemijo in kemijsko tehnologijo
|
|---|
| Opis: | In the present study, vortex-assisted matrix solid-phase dispersion (VA-MSPD) extraction
was used to isolate the major bioactive compounds from H. arenarium. To reduce the negative environmental impact of the conventionally used organic solvents, four different choline chloride-based
natural deep eutectic solvents (NADES) were investigated as possible eluents. The most influential
VA-MSPD extraction parameters: stationary phase (adsorbent), adsorbent/sample ratio, vortex time,
and volume of extraction solvent were systematically optimized. Ultrasound-assisted extraction
with 80% MeOH was used as the standard method for the comparison of results. The stability of the
obtained extracts was studied over a period of 0 to 60 days at three different temperatures (−18 ◦C,
4
◦C, and 25 ◦C). All extracts were evaluated both spectrophotometrically (determination of total
phenolic content (TPC) and antioxidant activity by ABTS and FRAP assay) and chromatographically
(HPLC-UV). NADES based on choline chloride and lactic acid (ChCl-LA) was selected as the most
effective extractant, with a determined TPC value of its extract of 38.34 ± 0.09 mg GA/g DW (27%
higher than the methanolic VA-MSPD extract) and high antioxidant activity. The content of individual phenolic compounds (chlorogenic acid, dicaffeoylquinic acid isomers, naringenin isomers,
and chalcones) in the ChCl-LA extract, determined by HPLC-UV, was comparable to that of the
conventionally obtained one. Moreover, the stabilization effect of ChCl-LA was confirmed for the
studied compounds: chlorogenic acid, naringenin-40
-O-glucoside, tomoroside A, naringenin-5-Oglucoside, isosalipurposide, and naringenin. The optimum VA-MSPD conditions for the extraction of
H. arenarium polyphenols were: florisil/sample ratio of 0.5/1, a vortex time of 2 min, and an elution
volume of ChCl-LA of 10 mL. |
|---|
| Ključne besede: | matrix solid phase dispersion extraction, MSPD, natural deep eutectic solvents, NADES, extraction optimization, sandy everlasting, phenolic compounds, stability studies, antioxidant activity |
|---|
| Status publikacije: | Objavljeno |
|---|
| Verzija publikacije: | Objavljena publikacija |
|---|
| Poslano v recenzijo: | 11.11.2022 |
|---|
| Datum sprejetja članka: | 06.12.2022 |
|---|
| Datum objave: | 11.12.2022 |
|---|
| Založnik: | MDPI |
|---|
| Leto izida: | 2022 |
|---|
| Št. strani: | 17 str. |
|---|
| Številčenje: | Vol. 11, iss. 24 |
|---|
| PID: | 20.500.12556/DKUM-92750  |
|---|
| UDK: | 66.02 |
|---|
| COBISS.SI-ID: | 134436867 |
|---|
| DOI: | 10.3390/plants11243468 |
|---|
| ISSN pri članku: | 2223-7747 |
|---|
| Avtorske pravice: | © 2022 by the authors |
|---|
| Datum objave v DKUM: | 09.05.2025 |
|---|
| Število ogledov: | 0 |
|---|
| Število prenosov: | 3 |
|---|
| Metapodatki: |    |
|---|
| Področja: | Ostalo
|
|---|
|
:
|
Kopiraj citat |
|---|
| | | | Skupna ocena: | (0 glasov) |
|---|
| Vaša ocena: | Ocenjevanje je dovoljeno samo prijavljenim uporabnikom. |
|---|
| Objavi na: |  |
|---|
Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše
podrobnosti ali sproži prenos. |
