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Title:Izolacija biološko aktivnih komponent iz Betonica officinalis
Authors:ID Dovnik, Urška (Author)
ID Knez Marevci, Maša (Mentor) More about this mentor... New window
ID Cör Andrejč, Darija (Comentor)
Files:.pdf UN_Dovnik_Urska_2024.pdf (2,18 MB)
MD5: FF4CB4AEA7F2D6B1F4DB86F7F66B254A
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Betonika (Betonica officinalis) ima zaradi svoje zdravilnosti široko paleto uporabe v zgodovini. Odločili smo se izolirati biološko aktivne komponente iz posušenih delov betonike in jih okarakterizirati. Pri tem smo uporabili dve različni ekstrakcijski metodi (maceracijo in UZ ekstrakcija) in topili etanol in voda ter njihove mešanice. Ugotavljali smo kako različne ekstrakcijske metode in topila vplivajo na izolacijo biološko aktivnih komponent iz betonike. Najvišje izkoristke ekstrakcij smo dosegli z vročo maceracijo (približno 14 %). Pridobljenim ekstraktom smo nato določili vsebnost totalnih fenolov, proantocianidinov in antioksidativni potencial. Totalne fenole smo določali s pomočjo Follin-Ciocalteu reagenta. Kot standardno raztopino smo uporabili galno kislino. Rezultate smo podali v mg GA na g ekstrakta oz. materiala. Vsebnost proantocianidinov v ekstraktih smo določili z uporabo Porter-Hrstich-Chantovo metode. Pri tem smo kot standard uporabili cianidin. Rezultate smo podali v mg PAC na g ekstrakta oz. materiala. Nenazadnje smo s pomočjo DPPH testa določili antioksidativni potencial izoliranih spojin. Rezultati analiz so pokazali, da betonika vsebuje od 1-13,4 mg GA/g materiala. Prisotnost proantocianidinov je med 0,09 in 0,77 mg PAC/g materiala. Test DPPH je pokazal, da antioksidativna aktivnost ekstraktov betonike niha med 16,7 % in 31%.
Keywords:ekstrakcija, betonika, totalni fenoli, proantocianidini, antioksidativnost
Place of publishing:Maribor
Year of publishing:2024
PID:20.500.12556/DKUM-90984 New window
Publication date in DKUM:02.12.2024
Views:0
Downloads:8
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:14.10.2024

Secondary language

Language:English
Title:Isolation of bioactive compounds from Betonica officinalis
Abstract:Wood betony or purple betony (Betonica officinalis) has been widely used throughout history for its medicinal properties. We decided to isolate bioactive compounds from the dried parts of purple betony and characterise them. Two different extraction methods were used (maceration and ultrasonic extraction) and the solvents ethanol and water and their mixtures. We analysed the effect of the different extraction methods and solvents on the isolation of the bioactive compounds in purple betony. The highest extraction yields were obtained with hot maceration (about 14 %). The extracts obtained were then analysed for total phenols, proanthocyanidins and their antioxidant potential. Total phenols were determined using the Follin-Ciocalteu reagent. Gallic acid was used as a standard solution. The results are expressed in mg GA per g extract or material. The extracts were also analysed for proanthocyanidins using the Porter-Hrstich-Chant method. Cyanidin was used as standard. The results are expressed in mg PAC per g of extract or material. We were also interested in the antioxidant potential of the isolated compounds, which was determined using the DPPH assay. The results of the analyses showed that wood betony contains between 1-13.4 mg GA/g material, of which the proportion of proanthocyanidins is between 0.09 and 0.77 mg PAC/g material. The DPPH assay showed that the antioxidant activity of the wood betony extracts varies between 16.7% and 31%.
Keywords:extraction, betony, total phenols, proanthocyanidins, antioxidant activity


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