| Naslov: | Adipose tissue gene expression of entire male, immunocastrated and surgically castrated pigs |
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| Avtorji: | ID Poklukar Žnidaršič, Klavdija (Avtor)
ID Čandek-Potokar, Marjeta (Avtor)
ID Vrecl, Milka (Avtor)
ID Batorek Lukač, Nina (Avtor)
ID Fazarinc, Gregor (Avtor)
ID Kress, Kevin (Avtor)
ID Stefanski, Volker (Avtor)
ID Škrlep, Martin (Avtor) |
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| Datoteke: |  Poklukar-2021-Adipose_Tissue_Gene_Expression_o.pdf (2,19 MB)
MD5: 935E2B64F5108913AE3468CA2E951EF8
 https://doi.org/10.3390/ijms22041768
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Znanstveno delo |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | FKBV - Fakulteta za kmetijstvo in biosistemske vede
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| Opis: | Differences in adipose tissue deposition and properties between pig male sex categories, i.e., entire males (EM), immunocastrates (IC) and surgical castrates (SC) are relatively well-characterized, whereas the underlying molecular mechanisms are still not fully understood. To gain knowledge about the genetic regulation of the differences in adipose tissue deposition, two different approaches were used: RNA-sequencing and candidate gene expression by quantitative PCR. A total of 83 differentially expressed genes were identified between EM and IC, 15 between IC and SC and 48 between EM and SC by RNA-sequencing of the subcutaneous adipose tissue. Comparing EM with IC or SC, upregulated genes related to extracellular matrix dynamics and adipogenesis, and downregulated genes involved in the control of lipid and carbohydrate metabolism were detected. Differential gene expression generally indicated high similarity between IC and SC as opposed to EM, except for several heat shock protein genes that were upregulated in EM and IC compared with SC. The candidate gene expression approach showed that genes involved in lipogenesis were downregulated in EM compared with IC pigs, further confirming RNA-sequencing results. |
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| Ključne besede: | pigs, adipose tissue, entire males, immunocastration, surgical castration, RNA-sequencing, expression |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Poslano v recenzijo: | 11.01.2021 |
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| Datum sprejetja članka: | 06.02.2021 |
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| Datum objave: | 10.02.2021 |
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| Založnik: | MDPI |
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| Leto izida: | 2021 |
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| Št. strani: | Str. 1-18 |
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| Številčenje: | Letn. 22, Št. 4, št. članka 1768 |
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| PID: | 20.500.12556/DKUM-90943  |
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| UDK: | 636.4 |
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| COBISS.SI-ID: | 50997507 |
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| DOI: | 10.3390/ijms22041768 |
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| ISSN pri članku: | 1422-0067 |
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| Datum objave v DKUM: | 10.10.2024 |
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| Število ogledov: | 0 |
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| Število prenosov: | 5 |
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| Metapodatki: |    |
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| Področja: | Ostalo
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