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Title:Sinteza membran in kroglic bakterijske nanoceluloze kot potencialnih sistemov za sproščanje bioaktivnih komponent
Authors:ID Tratnjek, Tara (Author)
ID Primožič, Mateja (Mentor) More about this mentor... New window
ID Leitgeb, Maja (Comentor)
ID Kupnik, Kaja (Comentor)
Files:.pdf MAG_Tratnjek_Tara_2024.pdf (4,65 MB)
MD5: ACBFF134210CA3AB72E52FD2EBD00E46
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Nanoceluloza, še posebej tista, ki izvira iz naravnih virov, kaže ogromen potencial za uporabo na različnih področjih, kot so toplotnoizolacijski materiali, biomedicinsko inženirstvo, embalaža, kompoziti itd. Raziskave in razvoj na tem področju se še naprej širijo, kar odpira nove možnosti za uporabo tega naravnega polimera v tehnologiji in medicini. V magistrski nalogi smo za sintezo bakterijske nanoceluloze (BNC) uporabili bakterijo rodu Komagataeibacter in proučevali vpliv različnih parametrov na produktivnost BNC. V eksperimentalnem delu smo z uporabo statičnega gojenja pridobili BNC v obliki membran, z uporabo dinamičnega gojenja pa BNC v obliki kroglic. Ugotovili smo, da produktivnost bakterije Komagataeibacter narašča z naraščanjem časa gojenja, medtem ko hitrost stresanja vpliva na število, premer in maso proizvedenih BNC kroglic. Za dosego protibakterijske učinkovitosti BNC kroglic smo le-te modificirali z naravnim ekstraktom iz koščic avokada ter proučili kinetiko sproščanja ekstrakta iz BNC kroglic. Z uporabo bioreaktorja je bil proces pridobivanja BNC kroglic prenesen tudi iz laboratorijskega v polindustrijsko merilo.
Keywords:bakterijska nanoceluloza, bioreaktor, FTIR, Komagataeibacter xylinus, SEM, protibakterijska aktivnost
Place of publishing:Maribor
Publisher:[T. Tratnjek]
Year of publishing:2024
PID:20.500.12556/DKUM-90063 New window
UDC:547.458.81.057:[604.4:615.281.9](043.2)
COBISS.SI-ID:221025539 New window
Publication date in DKUM:19.09.2024
Views:0
Downloads:14
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:21.08.2024

Secondary language

Language:English
Title:Synthesis of bacterial nanocellulose membranes and beads as potential release systems of bioactive compounds
Abstract:Nanocellulose, especially that originating from natural sources, already shows enormous potential for applications in various fields such as thermal insulation materials, biomedical engineering, packaging, composites, etc. Research and development in this area continues to expand, opening new possibilities for the use of this natural polymer in technology and medicine. In the master's thesis, we used bacteria of the genus Komagataeibacter for the synthesis of bacterial nanocellulose (BNC) and studied the influence of various parameters on the productivity of BNC. In the experimental part, we produced BNCs in the form of membranes using static cultivation and BNCs in the form of beads using dynamic cultivation. We found that the productivity of Komagataeibacter increases with longer cultivation time, while the shaking speed affects the number, diameter and mass of BNC beads produced. In order to achieve the antibacterial effectiveness of BNC beads, we modified them with a natural extract from avocado seeds and studied their release kinetics. Using a bioreactor, the process of producing BNC beads was also scaled up from a laboratory to a semi-industrial scale.
Keywords:bacterial nanocellulose, bioreactor, FTIR, Komagataeibacter xylinus, SEM, antibacterial activity


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