Title: | Use of emulsion-templated, highly porous polyelectrolytes for in vitro germination of chickpea embryos : a new substrate for soilless cultivation |
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Authors: | ID Majer Kovačič, Janja (Author) ID Ciringer, Terezija (Author) ID Ambrožič-Dolinšek, Jana (Author) ID Kovačič, Sebastijan (Author) |
Files: | Kovacic-2022-Use_of_Emulsion-Templated,_Highly.pdf (4,70 MB) MD5: 63C45CD6873FBB42DD02F668E7CBA680
https://doi.org/10.1021/acs.biomac.2c00593
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Language: | English |
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Work type: | Scientific work |
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Typology: | 1.01 - Original Scientific Article |
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Organization: | FNM - Faculty of Natural Sciences and Mathematics
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Abstract: | The application of highly porous and 3D interconnected microcellular polyelectrolyte polyHIPE (PE-PH) monoliths based on (3-acrylamidopropyl)-trimethylammonium chloride as soilless cultivation substrates for in vitro embryo culture is discussed. The embryo axes isolated from chickpea seeds are inoculated onto the surface of the monoliths and allowed to germinate. Germination study show that the newly disclosed PE-PH substrate performs much better than the conventionally used agar as the germination percentage, shoot and root length, fresh and dry weight as well as the number of leaves are enhanced. The PE-PHs exhibit a higher absorption capacity of the plant growth medium, that is, 36 g·g–1 compared to agar, that is, 20 g·g–1, and also survive autoclaving conditions without failing. The key advantage over standard agar substrates is that they can be reused several times and also without prior sterilization. These results suggest that PE-PHs with exceptional absorption/retention properties and robustness have great potential as soilless substrates for in vitro plant cultivation. |
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Keywords: | absorption, catalyst supports, germination, plants, porosity |
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Publication status: | Published |
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Publication version: | Version of Record |
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Submitted for review: | 11.05.2022 |
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Article acceptance date: | 21.06.2022 |
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Publication date: | 08.07.2022 |
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Publisher: | American Chemical Society |
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Year of publishing: | 2022 |
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Number of pages: | Str. 3452-3457 |
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Numbering: | Letn. 23, Št. 8 |
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PID: | 20.500.12556/DKUM-88723 |
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UDC: | 577.2 |
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ISSN on article: | 1525-7797 |
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COBISS.SI-ID: | 118269187 |
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DOI: | 10.1021/acs.biomac.2c00593 |
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Publication date in DKUM: | 15.07.2024 |
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Views: | 114 |
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Downloads: | 11 |
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Metadata: | |
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Categories: | Misc.
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