| Title: | Diversity and content of carotenoids and other pigments in the transition from the green to the red stage of Haematococcus pluvialis microalgae identified by HPLC-DAD and LC-QTOF-MS |
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| Authors: | ID Grujić, Jaša Veno (Author)
ID Todorović, Biljana (Author)
ID Kranvogl, Roman (Author)
ID Ciringer, Terezija (Author)
ID Ambrožič-Dolinšek, Jana (Author) |
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| Files: |  RAZ_Grujic_Jasa_Veno_2022.pdf (2,92 MB)
MD5: 1B553D913D58A379FCCB00BFADF18958
 https://www.mdpi.com/2223-7747/11/8/1026
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FNM - Faculty of Natural Sciences and Mathematics
PEF - Faculty of Education
FKBV - Faculty of Agriculture and Life Sciences
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| Abstract: | H. pluvialis is a unicellular freshwater alga containing many bioactive compounds, especially carotenoids, which are the strongest antioxidants among the pigments. This study evaluates
the composition and content of carotenoids and other pigments in both stages of algae life cycle,
especially in the green vegetative stage, less studied in comparison to the red stage. To determine
the composition and content of carotenoids, a combination of HPLC-DAD and LC-QTOF-MS was
used. The content of carotenoids in the green vegetative stage was significantly lower than in the
red vegetative stage. In the green vegetative stage, 16 different carotenoids and other pigments were
identified. Among the total 8.86 mg g−1 DW of pigments, 5.24 mg g−1 DW or 59% of them were
chlorophyll a with its derivatives, and 3.62 mg g−1 DW or 41% of them were free carotenoids. After
the transition from the green to the red stage, the carotenoid composition was replaced by secondary
carotenoids, astaxanthin and its esters, which predominated in the whole carotenoid composition.
In addition to free astaxanthin, 12 astaxanthin monoesters, 6 diesters and 13 other carotenoids were
determined. The majority of 37.86 mg g−1 DW pigments were monoesters. They represented 82% of
all pigments, and their content was about 5 times higher than both, diesters (5.91 mg g−1 DW or 12%
of all) and free carotenoids (2.4 mg g−1 DW or 6% of all). The results of the study contribute to the
data on the overall pigment composition and content of H. pluvialis algae and provide the basis for
further improvement of cultivation of the H. pluvialis algae.
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| Keywords: | antioxidants, astaxanthin, chlorophylls, bioactive compounds, algae, Haematococcus, life cycle, pigment composition, secondary carotenoids |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 15.03.2022 |
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| Article acceptance date: | 07.04.2022 |
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| Publication date: | 09.04.2022 |
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| Publisher: | MDPI |
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| Year of publishing: | 2022 |
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| Number of pages: | 14 str. |
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| Numbering: | Vol. 11, iss. 8 |
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| PID: | 20.500.12556/DKUM-88637  |
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| UDC: | 582.3 |
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| ISSN on article: | 2223-7747 |
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| COBISS.SI-ID: | 104399875 |
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| DOI: | 10.3390/plants11081026 |
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| Publication date in DKUM: | 21.05.2024 |
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| Views: | 172 |
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| Downloads: | 22 |
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| Metadata: |    |
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| Categories: | Misc.
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