| Naslov: | Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus ▫$AV436^T$▫ and Komagataeibacter xylinus LMG 1518 |
|---|
| Avtorji: | ID Gorgieva, Selestina (Avtor)
ID Jančič, Urška (Avtor)
ID Cepec, Eva (Avtor)
ID Trček, Janja (Avtor) |
|---|
| Datoteke: |  RAZ_Gorgieva_Selestina_2023.pdf (8,06 MB)
MD5: 2C73066B287F241767D911392EE6C56E
 https://doi.org/10.1016/j.ijbiomac.2023.125368
|
|---|
| Jezik: | Angleški jezik |
|---|
| Vrsta gradiva: | Članek v reviji |
|---|
| Tipologija: | 1.01 - Izvirni znanstveni članek |
|---|
| Organizacija: | FS - Fakulteta za strojništvo
FNM - Fakulteta za naravoslovje in matematiko
|
|---|
| Opis: | The microbial production of cellulose using different bacterial species has been extensively examined for various
industrial applications. However, the cost-effectiveness of all these biotechnological processes is strongly related to the culture medium for bacterial cellulose (BC) production. Herein, we examined a simple and modified
procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium
for BC production by acetic acid bacteria (AAB). The central composite design (CCD) was used to optimise the GP
hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents
(4.8 g/L). The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the
recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/
L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane). The
membranes were synthesized in only 4 days of bacteria culturing, 1 st day with shaking, followed by 3 days of
static incubation. The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes
made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose
allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile
modulus, and elongation, respectively. Here presented study is the first report on utilising a GP-hydrolysate
without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently
described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste
material. The scale-up protocol of the scheme presented here will be needed for the cost-optimisation of BC
production at the industrial levels. |
|---|
| Ključne besede: | grape pomace hydrolysate, bacterial cellulose, acetic acid bacteria, Komagataeibacter melomenusus |
|---|
| Status publikacije: | Objavljeno |
|---|
| Verzija publikacije: | Objavljena publikacija |
|---|
| Poslano v recenzijo: | 16.04.2023 |
|---|
| Datum sprejetja članka: | 11.06.2023 |
|---|
| Datum objave: | 15.06.2023 |
|---|
| Založnik: | Elsevier |
|---|
| Leto izida: | 2023 |
|---|
| Št. strani: | 16 str. |
|---|
| Številčenje: | Vol. 244, [article no.] ǂ125368 |
|---|
| PID: | 20.500.12556/DKUM-88194  |
|---|
| UDK: | 579.6 |
|---|
| COBISS.SI-ID: | 155994883 |
|---|
| DOI: | 10.1016/j.ijbiomac.2023.125368 |
|---|
| ISSN pri članku: | 0141-8130 |
|---|
| Datum objave v DKUM: | 26.07.2024 |
|---|
| Število ogledov: | 98 |
|---|
| Število prenosov: | 13 |
|---|
| Metapodatki: |    |
|---|
| Področja: | Ostalo
|
|---|
|
:
|
Kopiraj citat |
|---|
| | | | Skupna ocena: | (0 glasov) |
|---|
| Vaša ocena: | Ocenjevanje je dovoljeno samo prijavljenim uporabnikom. |
|---|
| Objavi na: |  |
|---|
Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše
podrobnosti ali sproži prenos. |
