Naslov: | Ensiling, in vitro rumen digestion and soaking in slurry altered the germination capacity of Rumex obtusifolius seeds |
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Avtorji: | ID Gselman, Anastazija (Avtor) ID Brus, Maksimiljan (Avtor) |
Datoteke: | Gselman-2023-Ensiling,_In_Vitro_Rumen_Digestio.pdf (293,08 KB) MD5: DFDF26D005B37D9B517A8D5EB1F5BA35
https://doi.org/10.3390/agriculture13071347
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Jezik: | Angleški jezik |
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Vrsta gradiva: | Znanstveno delo |
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Tipologija: | 1.01 - Izvirni znanstveni članek |
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Organizacija: | FKBV - Fakulteta za kmetijstvo in biosistemske vede
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Opis: | This study investigated whether the process of ensiling and in vitro digestion in rumen juice, as well as the response to soaking in pig or cattle slurry, affects the germination rate and germination energy of Rumex obtusifolius (broad-leaved dock) seeds. Seeds were subjected to different treatments (200 seeds each) in three experiments: (I) seed ensiling (8 weeks) followed by in vitro rumen digestion (24, 36 and 48 h); (II) the soaking of non-ensiled and ensiled seeds in cattle or pig slurry (2, 4 and 24 weeks); and (III) the in vitro rumen digestion (24, 36 and 48 h) of non-ensiled and ensiled seeds followed by soaking in cattle or pig slurry (24 weeks). The control treatment included untreated seed (0—non-ensiled seed; 0—no in vitro rumen digestion; and 0—no soaking in slurry). Germination tests (germination rate and germination energy) were then conducted in four replicates in the germination chamber under alternating day (20–35 °C for 14 h under light) and night conditions (17–20 °C for 10 h without light) at 75% relative humidity. Experiment I showed that ensiling significantly (p ≤ 0.001) reduced both the germination rate and germination energy of R. obtusifolius seeds. In addition, the length of in vitro digestion duration that the non-ensiled seeds were subjected to significantly (p ≤ 0.001) reduced their germination energy but not the total germination rate. However, the seeds that were subjected to the process of ensiling and in vitro digestion in the rumen lost their germination completely. The Experiment II investigated the effects of soaking non-ensiled seeds in slurry and showed that germination rates were comparable in pig and cattle slurry. Longer soaking times significantly reduced the germination rate, with no germination observed after 24 weeks. The Experiment III considered the combined effects of in vitro digestion and slurry soaking and showed that rumen digestion reduced the proportion of germinable seeds. Germination was inhibited in pig slurry, while in cattle slurry, a decreasing germination rate was observed with increasing digestion time. |
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Ključne besede: | broad-leaved dock, in vitro rumen digestion, ensiling, pig and cattle slurry, seed germination |
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Status publikacije: | Objavljeno |
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Verzija publikacije: | Objavljena publikacija |
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Poslano v recenzijo: | 25.05.2023 |
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Datum sprejetja članka: | 02.07.2023 |
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Datum objave: | 04.07.2023 |
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Založnik: | MDPI |
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Leto izida: | 2023 |
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Št. strani: | Str. 1-13 |
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Številčenje: | Letn. 13, Št. 7, št. članka 1347 |
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PID: | 20.500.12556/DKUM-88035 |
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UDK: | 633.2/.3:636.084 |
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COBISS.SI-ID: | 157642499 |
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DOI: | 10.3390/agriculture13071347 |
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ISSN pri članku: | 2077-0472 |
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Datum objave v DKUM: | 05.04.2024 |
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Število ogledov: | 302 |
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Število prenosov: | 7 |
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Metapodatki: | |
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Področja: | Ostalo
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