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Title:Vpliv hidrolatov damaščanske vrtnice (Rosa x damascena Mill.), sivke (Lavandula angustifolia Mill.) in laškega smilja (Helichrysum italicum (Roth) G. Don) na metabolno aktivnost človeških keratinocitov in melanomskih celic
Authors:ID Črnigoj, Neža (Author)
ID Dariš, Barbara (Mentor) More about this mentor... New window
ID Lipovšek, Saška (Comentor)
Files:.pdf MAG_Crnigoj_Neza_2023.pdf (3,52 MB)
MD5: 4FC6DBF46EFA2DC5A7D3F304EA11A077
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:Hidrolati so stranski produkti pri pridelavi eteričnih olj. Proučevali smo vpliv hidrolatov damaščanske vrtnice, sivke in laškega smilja v dodanih celičnih kulturah keratinocitov NHEK in melanomskih celic WM-266-4 na obliko celic in njihovo metabolno aktivnost. Pripravili smo gojišča za celični kulturi keratinocitov NHEK in melanomskih celic WM-266-4. Celice smo izpostavili raztopinam hidrolatov. S pomočjo svetlobne mikroskopije smo izvedli morfološko analizo, s testom WST-8 analizo metabolne aktivnosti. Z morfološko analizo smo ugotovili spremenjeno obliko keratinocitov NHEK v celičnih kulturah, v katere smo dodali hidrolat sivke in laškega smilja. Metabolna aktivnost keratinocitov NHEK se je znižala pri hidrolatu sivke in laškega smilja. Hidrolat damaščanske vrtnice ni imel vpliva na obliko in metabolno aktivnost keratinocitov NHEK, prav tako ni vplival na melanomske celice WM-266-4. Hidrolata sivke in laškega smilja sta vplivala na obliko melanomskih celic WM-266-4. Na obliko celic je imel hidrolat sivke večji vpliv. Hidrolata sivke in laškega smilja sta povzročila inhibicijo metabolne aktivnosti melanomskih celic WM-266-4.
Keywords:hidrolati, sivka, damaščanska vrtnica, laški smilj, melanom, melanociti in keratinociti
Place of publishing:Maribor
Publisher:[N. Črnigoj]
Year of publishing:2023
PID:20.500.12556/DKUM-86198 New window
UDC:616-006.81:57/59(043.2)
COBISS.SI-ID:174976259 New window
Publication date in DKUM:06.12.2023
Views:454
Downloads:33
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:FNM
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:09.11.2023

Secondary language

Language:English
Title:Effect of hydrolates ofrose rosa mill.),( lavandula angustifolia mill.)and immortelle( helichrysum italicum (roth) g. don)on the metabolic activity of human keratinocytes and melanoma cells
Abstract:Hydrolates are by-products of essential oil production. The effect of hydrolates of Damascene rose, lavender, and immortelle in added cell cultures of NHEK keratinocytes and WM-266-4 melanoma cells on cell shape and metabolic activity was investigated. Cell culture media for NHEK keratinocytes and WM-266-4 melanoma cells were prepared. The cells were exposed to hydrolat solutions. Morphological analysis was performed by light microscopy and metabolic activity was analysed by WST-8 assay. The morphological analysis revealed an altered shape of NHEK keratinocytes in cell cultures supplemented with lavender and immortelle hydrolates. The metabolic activity of NHEK keratinocytes decreased with lavender and immortelle hydrolates. Damascene rose hydrolat did not affect the shape and metabolic activity of NHEK keratinocytes, nor did it affect WM-266-4 melanoma cells. Lavender and immortelle hydrolates had no effect on the shape of WM-266-4 melanoma cells. Lavender hydrolates had a greater effect on cell shape. Lavender and immortelle hydrolates caused inhibition of the metabolic activity of WM-266-4 melanoma cells.
Keywords:hydrolates, lavender, damask rose, immortelle, melanoma, melanocytes and keratinocytes


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