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Title:Določanje rastlinskim hormonom podobnih snovi v organskem dodatku za izboljšanje zemlje Big start s pomočjo biotestov (Cucummis, Mung in Triticum) : magistrsko delo
Authors:ID Ovčar, Andreja (Author)
ID Ambrožič-Dolinšek, Jana (Mentor) More about this mentor... New window
ID Ciringer, Terezija (Comentor)
Files:.pdf MAG_Ovcar_Andreja_2023.pdf (1,41 MB)
MD5: B0CA15C1508060E5A0BA5BA6554EA80C
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:Komercialni pripravek za izboljšanje kakovosti zemlje Big start lahko vpliva na morfološko-funkcionalne poteze poljščin, pri čemer bi nekatere učinke lahko pripisali rastlinskim rastnim regulatorjem podobnim snovem. V magistrskem delu smo z uporabo biotestov preverjali, ali izbrani komercialni pripravek Big start vsebuje avsksinom podobne spojine in ali vsebuje citokininom podobne spojine. Za določanje vsebnosti rastlinskim rastnim regulatorjem podobnih snovi v preparatu smo uporabili Mung, Cucummis in Triticum biotest. Na osnovi biotestov Mung in Cucummis smo potrdili vsebnost avksinom podobnih spojin. V Mung biotestu je biološki pripravek Big start največjo avksinsko aktivnost izkazal pri koncentraciji 0,5 mL L-1, kar je ekvivalentno koncentraciji med 1,0 mg L-1 in 3,0 mg L-1 indol-3-butanojske kisline (IBA). Z uporabo Cucummis biotesta smo potrdili vsebnost avksinom podobnih snovi primerljivo koncentraciji med 1,0 mg L-1 in 2,0 mg L-1 IBA. Z uporabo biotesta Cucummis smo potrdili tudi vsebnost citokininom podobnih spojin v koncentraciji primerljivi od 0,0 mg L-1 do 0,5 mg L-1 6-benzilaminopurina (BAP). Nasprotno z izvedbo biotesta Triticum nismo potrdili vsebnosti citokininom podobnih spojin. Zaradi nizke specifičnosti biotestov bi bilo točnost rezultatov smiselno nadgraditi še z drugimi metodami dela, kot je masna spektrometrija v kombinaciji z označevanjem molekul s težkimi izotopi ali celičnimi markerji.
Keywords:biotest, Mung, Cucummis, Triticum, Big start
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[A. Ovčar]
Year of publishing:2023
Number of pages:X f., 53 str.
PID:20.500.12556/DKUM-84022 New window
UDC:633/635(043.2)
COBISS.SI-ID:150491651 New window
Publication date in DKUM:26.04.2023
Views:625
Downloads:43
Metadata:XML DC-XML DC-RDF
Categories:FNM
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:29.03.2023

Secondary language

Language:English
Title:Determination of plant hormones like substances in organic supplement for soil enrichment Big start with bioassays (Cucummis, Mung and Triticum)
Abstract:The commercial soil preperation Big start can affect the morphological-functional characteristics of crops, and some of the effects could be attributed to plant growth regulator-like substances. In the MSc thesis, the selected commercial formulation Big start was analysed by bioassays for the presence of auxin-like compounds and fort he presence of cytokinin-like compounds. Mung, Cucummis and Triticum bioassays were used to determine the levels of plant growth regulator-like compounds in the preparation. The content of auxin-like compounds was confirmed using the Mung and Cucummis bioassays. In the Mung bioassay, the Big start bioassay showed the highest auxin activity at a concentration of 0.5 mL L-1, which corresponds to a concentration between 1.0 mL L-1 and 3.0 mL L-1 indole-3-butanoic acid (IBA). Using the Cucummis bioassay, we confirmed the content of auxin-like substances corresponding to a concentration between 1.0 mL L-1 and 2.0 mL L-1 IBA. The Cucummis bioassay also confirmed the presence of cytokinin-like compound at concentration comparable to 0.0 mL L-1 to 0.5 mL L-1 6-benzylaminopurine (BAP). In contrast, the Triticum bioassay did not confirm the presence of cytokinin-like compounds. Because of the low specificity of the bioassays, the accuracy of the results could be improved by other methods such as mass spectrometry in combination with labeling of molecules with heavy isotopes or cell markers.
Keywords:biotest, Mung, Cucummis, Triticum, Big start


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