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Title:Fiziologija acinarnih celic trebušne slinavke; pomen kalcijevih signalnih poti v fizioloških in patofizioloških pogojih
Authors:ID Marolt, Urška (Author)
ID Potrč, Stojan (Mentor) More about this mentor... New window
ID Stožer, Andraž (Co-mentor)
Files:.pdf DOK_Marolt_Urska_2023.pdf (6,86 MB)
MD5: 8D809A1788D525F40498135D8F7AA16E
 
Language:Slovenian
Work type:Doctoral dissertation
Typology:2.08 - Doctoral Dissertation
Organization:MF - Faculty of Medicine
Abstract:Fiziologija in patofiziologija eksokrinega dela trebušne slinavke sta v tesni povezavi s spremembami znotrajcelične koncentracije kalcijevih ionov. Večina našega znanja o kalcijevih signalnih poteh v acinarnih celicah temelji na poskusih in vitro, kjer celice encimsko izoliramo, kar spremeni njihovo strukturo, fiziologijo in omeji naše razumevanje. Zaradi teh omejitev smo uvedli metodo tkivne rezine trebušne slinavke kot eksperimentalni model in situ. Ocenili smo sposobnost za preživetje in morfološke značilnosti acinarnih celic z uporabo testa preživetja celic (LIVE/DEAD assay), transmisijske elektronske mikroskopije in imunofluorescenčnih metod. V glavnem delu raziskave smo opredelili funkcionalni odziv acinarnih celic na stimulacijo z acetilholinom in ga primerjali z odzivi na cerulein. Spremembo znotrajcelične koncentracije kalcijevih ionov smo merili s konfokalno mikroskopijo. Pokazali smo, da so različni parametri kalcijevih oscilacij koncentracijsko odvisni in da je aktivnost precej dobro usklajena znotraj acinusov, ne pa med acinusi. Metoda tkivne rezine trebušne slinavke tako v naših očeh predstavlja pomemben nov in zanesljiv eksperimentalni model za oceno normalne in patološke morfologije in fiziologije acinarnih celic.
Keywords:trebušna slinavka, acinarna celica, kalcijeve oscilacije, metoda tkivne rezine, konfokalna mikroskopija
Place of publishing:Maribor
Year of publishing:2023
PID:20.500.12556/DKUM-82937 New window
COBISS.SI-ID:149742595 New window
Publication date in DKUM:19.04.2023
Views:599
Downloads:66
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:MF
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:09.09.2022

Secondary language

Language:English
Title:Physiology of Pancreatic Acinar Cells; the Importance of Calcium Signaling Pathways in Physiological and Pathophysiological Conditions
Abstract:The physiology and pathophysiology of the exocrine pancreas are closely related to changes in intracellular calcium concentration. Most of our knowledge of calcium signaling pathways in acinar cells is based on in vitro experiments where cells or acini are enzymatically isolated from their environment, which can change their structure and physiology and limit our understanding. Due to these limitations, the pancreatic tissue slice technique was introduced as an experimental in situ model. The viability and morphological characteristics of acinar cells within the tissue slice were assessed using the LIVE/DEAD assay, transmission electron microscopy, and immunofluorescence imaging. In the main part of the study, we define the response of acinar cells to acetylcholine stimulation and compare them with the responses to cerulein. Changes in intracellular calcium concentration were detected by confocal microscopy. We have shown that the different parameters of calcium oscillations are concentration-dependent and that the activity is rather well synchronized within acini but not between acini. The pancreatic tissue slice technique represents in our eyes an important, new and reliable experimental model for evaluating the normal and pathological morphology and physiology of acinar cells.
Keywords:pancreas, acinar cell, calcium oscillations, tissue slice technique, confocal microscopy


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