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Title:Razvoj metodologije za proučevanje korelacije med aktivacijo mitohondrijev in tvorbo bionano-kompozitov v pljučnih epitelijskih celicah po izpostavitvi nanomaterialom : magistrsko delo
Authors:ID Vajs, Tanja (Author)
ID Štrancar, Janez (Mentor) More about this mentor... New window
ID Kokot, Hana (Comentor)
Files:.pdf MAG_Vajs_Tanja_2022.pdf (11,80 MB)
MD5: D9D645C779A5B9E7886FB0598410B4C1
PID: 20.500.12556/dkum/6cbcf599-3132-4470-a80a-2f2a99b104d1
 
.zip MAG_Vajs_Tanja_2022.zip (15,52 MB)
MD5: 067E319B69DDC76666DDDD0425578240
PID: 20.500.12556/dkum/660c41ab-b0e2-4dfa-bca2-24361eb6bf64
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:V magistrskem delu smo preučili aktivno odzivanje celic in aktivacijo celičnih energijskih procesov ob karanteni izbranega nanomateriala – nanocevk titanovega dioksida (TiO2). Pri tem smo raziskovali povezavo med dolžino mitohondrijev in volumnom bionano-kompozitov celic LA-4 ob karanteni nanocevk TiO2 pri površinski dozi 1:10 (razmerje med površino celice in površino nanodelcev). Pri tem smo analizirali vsako celico posebej, s čimer smo pokazali variabilnost v dolžini mitohondrijev in nastankom bionano-kompozitov med posameznimi celicami, in s tem nadgradili dosedanje raziskave. V ta namen smo nadgradili obstoječo metodo detekcije bionano-kompozitov ter dodatno razvili primerno analizo za določanje njihovega volumna, poleg tega pa smo izboljšali obstoječe pristope za analizo dolžine mitohondrijev zato, da se zmanjša časovna zahtevnost slednje. Pokazali smo, da je po 3-urni inkubaciji celic z nanocevkami TiO2 (površinska doza 1:10) dolžina mitohondrijev primerljiva z negativno kontrolo, s časom izpostavljenosti nanocevkam TiO2 pa se dolžina mitohondrijev krajša – najkrajše mitohondrije smo opazili po 2-dnevni inkubaciji. V primerjavi s tem pa se povprečen volumen bionano-kompozitov povečuje z daljšim časom inkubacije celic z nanocevkami TiO2 in je največji po 2 dnevih inkubacije. Pokazali smo tudi, da je povprečno število bionano-kompozitov na celico po inkubaciji z nanocevkami TiO2 pri površinski dozi 1:10 najmanjše po 3 urah in največje po 1 dnevu, po 2 dnevih pa se povprečno število bionano-kompozitov ponovno zmanjša, saj se posamezni bionano-kompoziti, ki smo jih opazili po 1-dnevni inkubaciji združijo v večje bionano-kompozite. S tem delom smo dodatno osvetlili časovni potek vpliva nanocevk TiO2 na dolžino mitohondrijev in nastanek bionano-kompozitov na celični membrani celic LA-4, ter prikazali korelacijo med časovnim odzivom mitohondrijev in tvorbo bionano-kompozitov na posameznih celicah pri tej vrsti nanodelcev.
Keywords:bionano-kompoziti, nanocevke titanovega dioksida, dolžina mitohondrijev, fragmentacija mitohondrijev, fluorescenčna konfokalna mikroskopija
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[T. Vajs]
Year of publishing:2022
Number of pages:XVII, 135 f.
PID:20.500.12556/DKUM-81156 New window
UDC:543.645.4:620.26(043.2)
COBISS.SI-ID:101083907 New window
Publication date in DKUM:13.07.2022
Views:656
Downloads:61
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:FNM
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:18.01.2022

Secondary language

Language:English
Title:Methodology development to study the correlation between mitochondrial activation and assembly of bionano-composites in lung epithelial cells exposed to nanomaterials
Abstract:The master's thesis studies the active response of cells and the activation of cellular energy processes during the quarantining of a selected nanomaterial – titanium dioxide nanotubes (TiO2). We investigated the relationship between the length of mitochondria and the volume of bionano-composites of lung epithelial cells LA-4 when quarantining TiO2 nanotubes at a surface dose of 1:10 (ratio between cell surface and nanoparticle surface). Each cell was analyzed separately, thereby enabling us to show the variability in both the length of mitochondria and the formation of bionano-composites among individual cells, thus advancing previous research. For this purpose, we upgraded the existing method of bionano-composite detection and additionally developed a suitable analysis to determine their volume; moreover, we improved existing approaches for analyzing mitochondrial length in order to reduce their excessive time consumption. We showed that after 3 hours of incubating cells with TiO2 nanotubes (surface dose 1:10), the length of mitochondria was comparable to the negative control group. With longer exposure to TiO2 nanotubes, the length of the mitochondria shortened – the shortest mitochondria were observed after 2 days of incubation. In comparison, the average volume of bionano-composites increased during the incubation and was the highest after 2 days of incubation. On the other hand, the average number of bionano-composites per cell was the lowest after 3 hours of incubation, the highest after 1 day, and decreased again after 2 days as the individual bionano-composites combined into larger bionano-composites. This work additionally illuminates how TiO2 nanotubes influence mitochondrial length and bionano-composite formation on the membrane of LA-4 cells over time, and shows the correlation between mitochondrial response over time and bionano-composite formation on individual cells with this type of nanoparticles.
Keywords:bionano-composites, titanium dioxide nanotubes, mitochondrial length, mitochondrial fragmentation, fluorescence confocal microscopy


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