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Title:Določitev optimalnih pogojev superkritične ekstrakcije s programom Design Expert in bioaktivnih komponent arnike (Arnica montana) : magistrsko delo
Authors:ID Tržan, Primož (Author)
ID Knez Marevci, Maša (Mentor) More about this mentor... New window
ID Žitek Makoter, Taja (Comentor)
ID Rozman, Urška (Comentor)
Files:.pdf MAG_Trzan_Primoz_2021.pdf (3,75 MB)
MD5: E9A9C9CBB6A2CE54F2268B56667CB041
PID: 20.500.12556/dkum/b1254e18-67e6-48ce-a80f-dd085bcb6ea6
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Rastlinske bioaktivne spojine (antioksidanti, totalni fenoli, proantocianidini, flavonoidi, itd.) so sekundarni metaboliti, ki jih proizvajajo rastline in izzovejo farmakološke ali toksične učinke pri človeku in živalih. Postopek priprave in izbira topila vplivata na kvaliteto in kvantiteto ekstrakta oz. komponent v ekstraktu. V magistrski nalogi smo raziskali rastlino Arnico Montano, ki ima v farmacevtski in kozmetični industriji velik potencial. Osredotočili smo se na postopek superkritične ekstrakcije in določili optimalne pogoje superkritične ekstrakcije arnike s CO2 na podagi izrisanega eksperimentalnega načrta, programa Design Expert Pro 12. Ugotovili smo, da višja kot sta temperatura in tlak, višji je izkoristek ekstrakcije. Pridobljenim ekstraktom smo s spektrofotometrično metodo determinirali biološko aktivne komponente (antioksidanti, totalni fenoli, proantocianidini, totalni flavonoidi), katerih prisotnost naj bi vplivala na protivnetni, antiradikalni, antibakterijski, protiglivični in antioksidativni potencial. Rezultati so nam pokazali, da ekstrakti, ki so bili pridobljeni pri višji temperaturi ter tlaku, vsebujejo višje vrednosti biološko aktivnih komponent. Slednjim smo nato določali še protimikrobni potencial, ki smo ga potrdili le ekstraktu 8 z najvišjo vsebnostjo antioksidantov ter totalnih flavonoidov, pridobljenem pri tlaku 100 bar in temperaturi 60 C.
Keywords:gorska arnika, antioksidativnost, fenolne spojine, proantocianidini, protimikrobni potencial
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[P. Tržan]
Year of publishing:2021
Number of pages:XI, 40 str.
PID:20.500.12556/DKUM-80201 New window
UDC:604.4:615.33(043.2)
COBISS.SI-ID:77464323 New window
Publication date in DKUM:22.09.2021
Views:823
Downloads:161
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:KTFMB - FKKT
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Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:01.09.2021

Secondary language

Language:English
Title:Determination of optimal conditions of supercritical extraction with program design expert and bioactive components of arnica (arnica montana)
Abstract:Plant bioactive compounds (antioxidants, total phenols, proanthocyanidins, flavonoids, etc.) are secondary metabolites produced by plants and cause pharmacological or toxic effects in humans and animals. The preparation process and the choice of solvent affect the quality and quantity of the extract or. components in the extract. In our master's thesis, we researched the Arnico Montano plant, which has great potential in the pharmaceutical and cosmetic industries. We focused on the supercritical extraction process and determined the optimal conditions for supercritical extraction of arnica with CO2 on the basis of a plotted experimental plan, Design Expert Pro 12. We found that the higher the temperature and pressure, the higher the extraction efficiency. The obtained extracts were determined by the spectrophotometric method of biologically active components (antioxidants, total phenols, proanthocyanidins, total flavonoids), the presence of which is expected to affect the anti-inflammatory, antiradical, antibacterial, antifungal and antioxidant potential. The results showed us that the extracts obtained at higher temperature and pressure contain higher values of biologically active components. The latter were then determined for their antimicrobial potential, which was confirmed only in extract 8 with the highest content of antioxidants and total flavonoids, obtained at a pressure of 100 bar and a temperature of 60 C.
Keywords:mountain arnica, antioxidant activity, phenolic compounds, proanthocyanidins, antimicrobial potential


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