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Naslov:Razvoj in karakterizacija nanovlaken s kapsuliranimi probiotiki : magistrsko delo
Avtorji:ID Slapničar, Špela (Avtor)
ID Simonič, Marjana (Mentor) Več o mentorju... Novo okno
ID Fras Zemljič, Lidija (Komentor)
ID Peršin, Zdenka (Komentor)
Datoteke:.pdf MAG_Slapnicar_Spela_2021.pdf (3,27 MB)
MD5: 5AF90AACF978609AD498C45952BF2A53
PID: 20.500.12556/dkum/9a5ce585-49ab-4d0e-9148-581712b15042
 
Jezik:Slovenski jezik
Vrsta gradiva:Magistrsko delo/naloga (mb22)
Tipologija:2.09 - Magistrsko delo
Organizacija:FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Opis:Probiotiki so živi mikroorganizmi, ki so koristni za gostitelja za preprečevanje rasti potencialno škodljivih bakterij ter za izboljšanje imunskega odziva. Razvoj nanovlaken s kapsuliranimi probiotiki je razmeroma nov postopek, saj nanovlakna predstavljajo nov in učinkovit dostavni sistem za probiotike. Eksperimentalni del je zajemal pripravo in karakterizacijo posameznih raztopin polimerov (PEO, NaALG) ter izbiro ustrezne koncentracije probiotika (svežega in liofiliziranega), ki smo ga nato dodali v polimerno raztopino (NaAlg:PEO v volumskem razmerju 1:1, oznaka PA). Za določitev optimalnih parametrov elektropredenja smo raztopinam PEO, PA, PA z inulinom (PAI), PA z liofiliziranim probiotikom (PAP), PA z inulinom in liofiliziranim probiotikom (PAIP) ter PA z inulinom in svežim probiotikom (PAIPs) izmerili prevodnost, viskoznost, pH ter površinsko napetost. Raztopine PAI, PAIP in PAIPs vsebujejo inulin, ki deluje kot prebiotik in smo ga dodali, da izboljša preživelost probiotika Lactobacillus paragasseri K7. Učinkovitost formiranih nanovlaken smo preverili z vrstičnim elektronskim mikroskopom (SEM), ujetje probiotika v nanovlakna pa smo dokazovali s Fourierjevo infrardečo (FT-IR) ter rentgensko fotoelektronsko spektroskopijo (XPS). Antioksidativen potencial vgrajenega probiotika v nanovlaknih smo preverjali z indirektno metodo določanja z ABTS radikalom ((2,2'-azino-bis(3-etilbenzotiazolin-6-sulfonska kislina)). Efektivnost učinkovanja sproščenega probiotika v različnih časovnih intervalih smo spremljali z metodo sproščanja probiotika, z metodo cone inhibicije pa smo preverili protimikrobno učinkovitost. Ugotovili smo, da se pri dodatku 3*1010 liofiliziranega probiotika L. paragasseri K7 v 20 ml polimerne raztopine, nanovlakna niso formirala, zaradi visoke prevodnosti. Dokazali smo, da se z nižanjem pH zniža tudi viskoznost polimerne raztopine z liofiliziranimi probiotikom L. paragasseri K7. Pri dodatku svežega probiotika v polimerno raztopino NaALG:PEO ter inulina (PAIPs) se nanovlakna niso formirala. Pri ostalih polimernih raztopinah so se nanovlakna tvorila, najdebelejša pri raztopini PAIP (0,8-1,6 µm), najtanjša pri PA (0,1-0,3 µm). Rezultati so pokazali, da kompozitni material PAP, PAIP, PAIPs ni antioksidativen. Liofiliziran probiotik L. paragasseri K7 sam po sebi je antioksidativen, saj je njegova IEt kar 100,0 %. Pri analizi sproščanja probiotika se je najbolje izkazal napreden material PAP z liofiliziranim probiotikom, vsi trije analizirani kompozitni materiali (PAP, PAIP, PAIPs) so v kratkem času sprostili ves ujet probiotik. Za raztopino PAIPs smo naredili še preizkus inhibicije rasti testnih bakterij (Escherichia coli ter Staphylococcus aureus), vendar nismo zaznali cone inhibicije. Iz rezultatov lahko sklepamo, da je bil razvoj nanovlaken uspešen. Uporaba nanovlaken s kapsuliranim probiotikom je bolj primerna za medicinske materiale s krajšim zahtevanim časovnim sproščanjem, do 24 ur. Izdelani produkti bi bili primerni za zdravljenje ran, tampone ter kot dostavni sistem v prehrani – zagotovi enostaven vnos polprodukta v končni izdelek.
Ključne besede:probiotiki, elektropredenje, nanovlakna, Lactobacillus paragasseri K7, antioksidativnost, inhibicija rasti testnih bakterij
Leto izida:2021
Kraj izvedbe:Maribor
Založnik:[Š. Slapničar]
Št. strani:XII, 61 str.
Izvor:Maribor
PID:20.500.12556/DKUM-80159 Novo okno
UDK:620.3:602.3(043.2)
COBISS.SI-ID:77459715 Novo okno
Datum objave v DKUM:22.09.2021
Število ogledov:429
Število prenosov:112
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
Področja:KTFMB - FKKT
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Licence

Licenca:CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
Opis:Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.
Začetek licenciranja:31.08.2021

Sekundarni jezik

Jezik:Angleški jezik
Naslov:Development and characterisation of nanofibers with encapsulated probiotics
Opis:Probiotics are live microorganisms which provide the host with beneficial effects on the inhibition of growth of potentially harmful bacteria and enhancement of the immune response. The development of nanofibers with capsulated probiotics is a relatively new procedure as they present a new and effective delivery system for probiotics. The experimental part encompassed the preparation and characterization of individual polymer solutions (PEO, NaALG) and selection of suitable probiotic concentration (fresh and lyophilized) which were then added to the polymer solution (NaAlg:PEO in 1:1 ratio, label PA). To determine optimal electrospinning parameters we measured the conductivity, viscosity, pH and surface tension of PEO, PA, PA with inulin (PAI), PA with lyophilized probiotic (PAP), PA with inulin and lyophilized probiotic (PAIP) and PA with inulin and fresh probiotic (PAIPs) solutions. The PAI (PA-inulin), PAIP (PA+inulin+lyophilized probiotic) and PAIPs (PA+inulin+fresh probiotic) solutions contain inulin, which acts as a prebiotic and was added to enhance the survival rate of probiotic Lactobacillus paragasseri K7. The effectiveness of forming nanofibers was studied with scanning electron microscope (SEM), the probiotic trapped in nanofibers were proven by Fourier infrared (FT-IR) and X-ray photoelectron spectroscopy (XPS). The antioxidative potential of probiotic built into nanofibers was studied by indirect determination method with ABTS radical ((2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)). The level of efficacy of releasing probiotic in different time intervals was studied by the method of releasing probiotics and the antimicrobial efficacy was examined with the zone of inhibition method. We have determined that the nanofibers did not form when adding 3*1010 lyophilized probiotic L. paragasseri K7 to 20 ml of polymer solution due to high conductivity. We have proven that the viscosity of lyophilized probiotic K7 polymer solution reduces with the lowering of pH. The nanofibers were not formed with added fresh probiotic into polymer solution NaALG:PEO and inulin solution (PAIPs). In the other polymer solutions the nanofibers had formed, the thickest in PAIP solution (0.8-1.6 µm) and the thinest in PA solution (0.1-0.3 µm). The results conclude that the composite material PAP, PAIP, PAIPs is not antioxidative. Lyophilized probiotic L. paragasseri K7 is antioxidative by itself as its IEt reaches 100,0%. In the analysis of releasing probiotic the advanced material PAP with lyophilized probiotic has proven to be the best, all three analysed composite materials (PAP, PAIP, PAIPs) have released all of the trapped probiotic in a short period of time. We also performed the growth inhibition test of testing bacteria (Escherichia coli and Staphylococcus aureus) for the PAIPs solution but the inhibition zone was not detected.   We can conclude from the results that the development of nanofibers was successful. The use of nanofibers with encapsulated probiotic is more suitable for medical materials with shorter required time release, up to 24 hours. The manufactured products would be more suitable for the treatment of wounds, tampons and as a nutritional delivery system– it ensures an easy intake of the intermediate good into the final product.
Ključne besede:probiotics, electrospinning, nanofibers, Lactobacillus paragasseri K7, antioxidative, growth inhibition test of testing bacteria


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