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Title:Sinteza s siliko funkcionaliziranih magnetnih nanodelcev za imobilizacijo proteinov : magistrsko delo
Authors:Degen, Aljaž (Author)
Ban, Irena (Mentor) More about this mentor... New window
Stergar, Janja (Co-mentor)
Files:.pdf MAG_Degen_Aljaz_2021.pdf (1,48 MB)
MD5: DC68E97433012608EEBFBBEE8BE5389B
 
Language:Slovenian
Work type:Master's thesis/paper (mb22)
Typology:2.09 - Master's Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Pri tem magistrskem delu smo pripravljali funkcionalizirane magnetitne (Fe3O4) nanodelce, ki bi lahko bili uporabljeni za imobilizacijo encimov tekom procesa čiščenja odpadne vode. Sintetizirane magnetitne nanodelce smo najprej funkcionalizirali s silikatno prevleko (SiO2) in (3-aminopropil) trietoksisilanom (APTES), na koncu je sledila še aktivacija z glutaraldehidom. Za karakterizacijo sintetiziranih magnetitnih nanodelcev in njihovih derivatov smo uporabili več različnih metod. Sestavo magnetitnih nanodelcev smo določali z rentgensko praškovno difrakcijo (RTG); za določitev čistosti pripravljenih nanodelcev in vsebnosti vlage na njihovi površini smo uporabili termogravimetrično analizo (TGA); velikost nanodelcev smo določili s pomočjo rentgenske praškovne difrakcije, z dinamičnim sipanjem svetlobe (DLS) in s transmisijsko elektronsko mikroskopijo (TEM); uspešno funkcionalizacijo derivatov magnetitnih nanodelcev smo določali z meritvami zeta potenciala, infrardečo spektroskopijo s Fourierjevo transformacijo (FTIR) in transmisijsko elektronsko mikroskopijo. Tekom magistrskega dela smo preverjali tudi, ali je potrebno za vezavo APTES-a na magnetitne nanodelce uporabiti silikatno prevleko (posredna vezava), ali lahko dobimo zadovoljive rezultate brez njene uporabe (direktna vezava). Uspeli smo uspešno sintetizirati in funkcionalizirati magnetitne nanodelce ustrezne velikosti s silikatno prevleko in APTES-om. Razlika med vzorcema, ki sta imela direktno in posredno vezan APTES, je minimalna, zato lahko korak funkcionalizacije nanodelcev s silikatno prevleko izpustimo. Uspela je tudi aktivacija nanodelcev z glutaraldehidom, torej so takšni nanodelci pripravljeni za imobilizacijo encimov.
Keywords:Fe3O4 nanodelci, silikatna prevleka, (3-aminopropil) trietoksisilan (APTES), glutaraldehid, imobilizacija encimov
Year of publishing:2021
Place of performance:Maribor
Publisher:[A. Degen]
Number of pages:XII, 55 f.
Source:Maribor
UDC:620.179.141:620.3
COBISS_ID:66729987 New window
Views:103
Downloads:17
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:KTFMB - FKKT
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:20.04.2021

Secondary language

Language:English
Title:Synthesis of silica functionalized magnetic nanoparticles for protein immobilization
Abstract:In this master's thesis, we prepared functionalized magnetite (Fe3O4) nanoparticles that could be used for enzyme immobilization during the wastewater treatment process. The synthesized magnetite nanoparticles were first functionalized with a silicate coating (SiO2) and (3-aminopropyl) triethoxysilane (APTES), followed by activation with glutaraldehyde. Several different methods were used to characterize the synthesized magnetite nanoparticles and their derivatives. The composition of magnetite nanoparticles was determined by X-ray powder diffraction (RTG); thermogravimetric analysis (TGA) was used to determine the purity of the prepared nanoparticles and the moisture content on their surface; the nanoparticle size was determined by X-ray powder diffraction, dynamic light scattering (DLS), and transmission electron microscopy (TEM); the successful functionalization of magnetite nanoparticle derivatives was determined by zeta potential measurements, Fourier-transform infrared spectroscopy (FTIR) and transmission electron microscopy. During the master's thesis, we also checked whether it is necessary to use a silicate coating to bind APTES to the magnetite nanoparticles (indirect bonding), or if satisfactory results can be obtained without using it (direct bonding). We were able to successfully synthesize and functionalize magnetite nanoparticles of appropriate size with a silicate coating and APTES. The difference between the samples that had directly and indirectly bound APTES is minimal, so the step of functionalization of nanoparticles with a silicate coating can be omitted. Activation of nanoparticles with glutaraldehyde was also successful, so such nanoparticles are ready to be used for enzyme immobilization.
Keywords:Fe3O4 nanoparticles, silicate coating, (3-Aminopropyl) triethoxysilane (APTES), glutaraldehyde, enzyme immobilization


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