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Title:Osamitev in identifikacija psihrofilnih in psihrotolerantnih bakterij in gliv iz Triglavskega ledenika in ledenika pod Skuto
Authors:Jarčič, Maša (Author)
Gunde-Cimerman, Nina (Mentor) More about this mentor... New window
Trček, Janja (Co-mentor)
Files:.pdf MAG_Jarcic_Masa_2021.pdf (3,15 MB)
MD5: A5E5044D37D42CF3484121E7C3E4E5E1
 
Language:Slovenian
Work type:Master's thesis/paper (mb22)
Typology:2.09 - Master's Thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:Ledeniki so dolgo veljali za abiotska okolja. Novejše raziskave pa kažejo, da so ledeniki posebni in dinamični mikrobni habitati, kjer uspevajo različni psihrofilni in psihotolerantni mikroorganizmi. Zaradi globalnega segrevanja se visokogorski ledeniki pospešeno talijo. S tem izginjajo okolja, v katerih so se preko tisočletij uspešno razvijale mikrobne združbe v relativno stabilnih pogojih. Mikroorganizmi, ki naseljujejo ledenike, so razvili vrsto prilagoditev na življenje pri nizkih temperaturah in na ciklična zamrzovanja in odtaljevanja. V Sloveniji trenutno obstajata dva ledenika. Triglavski ledenik leži v Julijskih Alpah, na severovzhodnem pobočju najvišjega vrha v državi, Triglava. Ledenik pod Skuto leži dobrih petdeset kilometrov vzhodneje ter nekaj 100 m južneje od Triglavskega ledenika. Vzorčenje na obeh ledenikih je potekalo v septembru 2018. Iz obeh ledenikov smo aseptično odvzeli tri vzorce ledu iz treh različnih lokacij, jih stalili in filtrirali ter filtre inkubirali na štirih različnih gojiščih primernih za rast gliv ter na enem gojišču za bakterije pri temperaturi 15 °C. Skupno smo osamili 181 glivnih izolatov, ki se uvrščajo v 19 rodov ter 66 bakterijskih izolatov, ki se uvrščajo v 22 rodov.
Keywords:Triglavski ledenik, ledenik pod Skuto, ledeniki, psihrofili, psihrotoleranti, mikroorganizmi, bakterije, glive, izolacija, identifikacija, PCR, ITS.
Year of publishing:2021
Publisher:[M. Jarčič]
Source:Maribor
UDC:579.24/26:577.2(497.4)(043.2)
COBISS_ID:56658947 New window
NUK URN:URN:SI:UM:DK:ZV9GGODM
Views:65
Downloads:6
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:FNM
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:12.02.2021

Secondary language

Language:English
Title:Isolation and identification of psychrophilic and psychrotolerant bacteria and fungi from triglav and skuta glacier
Abstract:Glaciers were long known as abiotic environments, but latest reseach shows that they are special and dynamic habitats where various psychrofilic and psychrotolerant organisms can thrive. Due to global warming highland glaciers are rapidly thawing, releasing enclosed cold-adapted microbial communities into the environment. Since glacial microbial communities were growing in relatively stable conditions, characterized by low temperature, few nutrients, surface exposure to UV-radiation, increased salinity in brine channels around ice crystals, many can't survive these changes. In Slovenia, only two glaciers (glacierets) remain. Triglav glacier is located in Julian Alps, on the northeast slope of the country's highest peak, Mt. Triglav. Skuta glacier, a bit further to the east is located in Kamnik-Savinja Alps. Sampling of ice was conducted on both glaciers in September 2018. Three samples on different locations on each glacier were taken and brought to the laboratory for further analyses. The samples were aseptically thawed and filtrated through membrane filters and incubated on different selective microbial culture media with temperature of 15 °C. We succsefully isolated and identificated 181 strains of fungi belonging to 19 genera and 66 bacterial strains belonging to 22 genera.
Keywords:Triglav glacier, Skuta glacier, glaciers, psychrophilic microorganisms, psychrotolerant microorganisms, bacteria, fungi, isolation, identification, PCR, ITS.


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