|Abstract:||Phthalates are esters of 1,2-benzenedikarboxylic acid. The products, produced from phthalates, are most commonly softeners, that improve flexibility and usability of polymers with high molecular mass. Linear and branched phthalate esters are used in production of plastics. We can find them in inks, colors, glues, flooring, animal products, cosmetics and pharmaceutical products. Phthalates are endocrine disruptors, beacuse they affect on normal hormonal activity in human body. Exposure to some pthalates can among other things cause anomalies in development of fetus during pregnancy, preeclampsia and early labour, it is also conected with problems in neurological development, lower semen quality, abortion, asthma, allergy and obesity. Humans can be exposed to them with inhalation, absorption through skin and ingestion. Absorption, metabolism, distribution and elimination of chemicals are dependent on their entrance into body.
In diploma thesis, we have developed an effective and short method with adequate results for determination of diethyl and dibutyl phthalate in blood serum and urine. Diethyl phtalate is commonly found in products fot personal care (fragrances), tablet coatings in pharmacy, colors and insecticides. Dibutyl phtalate is used in cellulose-acetate plastics, products for personal care (polish, cosmetics), varnish and tablet coatings. For extraction of aforementioned phthalates from biological matrices we used liquid-liquid extraction and for determination gas chromatography with mass spectrometry (GC-MS).|