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Title:Izolacija keratina iz odpadne volne s hidrotermičnimi postopki : magistrsko delo
Authors:Verdnik, Aleksandra (Author)
Škerget, Mojca (Mentor) More about this mentor... New window
Čolnik, Maja (Co-mentor)
Files:.pdf MAG_Verdnik_Aleksandra_2020.pdf (3,85 MB, This file will be accessible after 30.12.2021)
MD5: 5DCDF1A54CC454559746497C409753A7
 
Language:Slovenian
Work type:Master's thesis/paper (mb22)
Typology:2.09 - Master's Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Naraščajoča skrb za okolje in vse večje povpraševanje po varnih in trajnostnih bioloških materialih spodbujajo iskanje boljših in zelenih metod za izkoriščanje razpoložljivih naravnih stranskih proizvodov. Odpadna ovčja volna z visoko vsebnostjo keratina (95 %) je le eden izmed stranskih proizvodov, ki nastaja v tekstilni industriji. Smiselno bi bilo biorazgradljiv volneni keratin pretvoriti v komercialne biomateriale in jih uporabiti v nadaljnjih aplikacijah kot sekundarni proizvod. Pri tem ekstrakcija keratina iz odpadne volne s podkritično vodo predstavlja pomembno alternativo konvencionalnim procesom ekstrakcije. V okviru magistrskega dela smo iz odpadne volne izolirali keratin z uporabo podkritične vode. Izolacijo smo izvedli v visokotlačnem in visokotemperaturnem šaržnem reaktorju pri temperaturah med 150 °C in 250 °C in reakcijskem času od 5 min do 75 min. Najvišji izkoristek keratina smo dobili pri temperaturi 180 °C in času 60 min. S Fourierevo transformacijsko infrardečo spektroskopijo smo potrdili prisotnost keratina v dobljenih produktih izolacije. Izoliranemu keratinu smo nato določili molekulsko maso z dvema različnima metodama; s poliakrilamidno gelsko elektroforezo v prisotnosti natrijevega dodecil sulfata (SDS-PAGE elektroforeza) in z uporabo gelske kromatografije. Molekulska masa keratina, nastalega pri reakciji izolacije, je med 4 kDa in 13 kDa. Molekulske mase keratina padajo z naraščanjem temperature.
Keywords:odpadna volna, keratin, izolacija, podkritična voda, gelska kromatografija, elektroforeza
Year of publishing:2020
Place of performance:Maribor
Publisher:[A. Verdnik]
Number of pages:XII, 50 str.
Source:Maribor
UDC:66.061.34:[547.962.9:677.318](043.2)
COBISS_ID:32134659 New window
NUK URN:URN:SI:UM:DK:PZPG8PZB
Views:201
Downloads:0
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:KTFMB - FKKT
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:20.08.2020

Secondary language

Language:English
Title:Isolation of keratin from waste wool using hydrothermal processes
Abstract:The growing concern regarding the environment and the increasing demand for safe and sustainable biological materials are encouraging the search for better green methods of using available natural side products. Waste sheep wool with a high content of keratin (95 %) is just one of the side products that is created in the textile industry. It would be sensible to biodegrade the woollen keratin and transform it into commercial biomaterials which can be used in future applications as a secondary product. Through this the extraction of keratin from wastewater with sub-critical water represents an important alternative to the conventional processes of extraction. In the masters dissertation the keratin from the waste wool has been isolated by using sub-critical water. The isolation took place in a high-pressure and high-temperature reactor between the temperatures of 150 °C and 250 °C and a reaction time between 5 min to 75 min. The highest level of keratin was achieved at 180 °C and 60 min. with Fourjer’s transformation infrared spectroscopy we confirmed the presence of keratin in the obtained isolation products. The molecular mas of the obtained keratin was determined by using two different methods: with a polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE electrophoresis) and by using a gel-based chromatography. The molecular mass of keratin created by the isolation is between 4 kDa and 13 kDa. The molecular masses of keratin decrease with increasing of temperature.
Keywords:waste wool, keratin, isolation, subcritical water, gel chromatography, electrophoresis


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