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Title:Vpliv v gojišče dodanega 6-benzilaminopurina (BAP) na rast in razraščanje marelice v tkivni kulturi
Authors:ID Hribernik, Tamara (Author)
ID Šiško, Metka (Mentor) More about this mentor... New window
ID Kraner Šumenjak, Tadeja (Co-mentor)
Files:.pdf UN_Hribernik_Tamara_2019.pdf (1,89 MB)
MD5: 562F6491A30A7F21788C044EA7943CF6
Work type:Bachelor thesis/paper (mb11)
Typology:2.11 - Undergraduate Thesis
Organization:FKBV - Faculty of Agriculture and Life Sciences
Abstract:V letu 2019 smo na Fakulteti za kmetijstvo in biosistemske vede izvedli poskus pri katerem smo ugotavljali vpliv v gojišče dodanega citokinina 6-benzilaminopurina (BAP) na rast in razraščanje marelice v tkivni kulturi. V poskus so bili vključeni 103 poganjki marelice. Preizkušali smo tri različna gojišča. Gojišče G0 je predstavljalo kontrolo (brez dodanih hormonov), gojišču G1 smo dodali 0,01 mg/L NAA in 0,5 mg/L BAP, gojišču G2 pa smo dodali 0,01 mg/L NAA in 1 mg/L BAP. Ugotovljeno je bilo, da je dodani BAP statistično značilno vplival na prirast mase marelice. Pri G0 je bil po 9 tednih prirast mase 0,096 g ± 0,057, pri G1 1,073 g ± 0,838 in pri G2 2,257 g ± 1,140. Ugotovili smo tudi, da dodani BAP pozitivno vpliva na število novo nastalih poganjkov. Po 9 tednih namreč na gojišču G0 ni bilo opaženih novih poganjkov, na G1 je bilo 11,320 ± 5,881 poganjkov, na G2 pa 21,180 ± 9,612 poganjkov. Višja koncentracija BAP ni statistično značilno povečala števila vitrificiranih rastlin, se je pa z večjo koncentracijo BAP, povečal delež vitrificiranih rastlin. Delež vitrificiranih rastlin na G1 je znašal 20,6 %, na G2 35,3 %, na G0 (brez BAP) pa vitrificiranih rastlin ni bilo.
Keywords:marelica, Prunus armeniaca, mikropropagacija, BAP
Year of publishing:2019
Publication date in DKUM:22.08.2019
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License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:13.08.2019

Secondary language

Title:Effects of 6-benzylaminopurine (BAP) Added to the Culture Medium on Growth Characteristics of Apricot Tissue Cultures
Abstract:In the experiment, that was carried out in 2019, at the Faculty of Agriculture and Life Sciences, we studied the effects of 6-benzylaminopurine (BAP) added to the culture medium on growth characteristics of apricot tissue cultures. In the experiment we included 103 apricot shoots which were tested in three different media: G0, G1, G2. The G0 medium (the check medium) contained no plant growth regulators. To the G1 medium, we added 0.01 mg/L NAA and 0.5 mg/L BAP, while to the G2 medium we added 0.01 mg/L NAA and 1 mg/L BAP. The study showed that the added BAP had a significant impact on the increase of apricot plantlet weight. After 9 weeks, we documented the following increases of weights: 0.096 g ± 0.057 in the case of G0, 1.073 g ± 0.838 in the case of G1 and 2.257 g ± 1.140 in the case of G2. We also concluded that the added BAP had a positive effect on the number of newly formed shoots. After 9 weeks, no new shoots were noticed on G0, while on G1 we noticed 11.320 ± 5.881 shoots and on G2 21.180 ± 9.612 shoots. With higher concentrations of BAP, we did not significantly increase the number of vitrified plants, however, we were able to increase the percentages of vitrified plants. On G1 medium it was 20.6%, on G2 35.3% and on G0 (without BAP) 0%.
Keywords:apricot, Prunus armeniaca, micropropagation, BAP


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