|Abstract:||The purpose of the study was to carry out pre-treatment of chicken manure with bedding which is from a mixture of straw and sawdust. In order to improve the C/N ratio, straw has been added to the mixture of chicken manure with bedding in ratios 80:20 and 60:40 (except 100:0 mixture, which was without added straw). Pre-treatment was carried out with two types of white rot fungi, Bjerkandera adusta (BJA), commonly known as the smoky polypore or smoky bracket and Flammulina velutipes (FL), also known by the name golden needle mushroom, futu mushroom or lily mushroom. Throughout the study, the amounts of CO₂ released was measured in various fungus pre-treated mixtures at different pre-treatment times and with various proportions of manure and straw. In well-overgrown mixtures by fungi, the proportions of lignin, carbohydrates and extracted material were monitored. As it is stated in the literature that fungi degrade lignocelluloses into monosaccharides glucose, xylose, mannose, galactose, ramnose and arabinose, the presence of monosaccharides was also monitored for non-hydrolysed and hydrolysed material overgrown by fungus BJA. To monitor the amounts of released CO₂ and consumed oxygen, CO₂ and O₂ sensors and a gas chromatograph were used.
It was discovered that CO₂ was released, but the growth of fungi was uneven, and consequently also the production of CO₂ was uneven. More CO₂ was released by the BJA fungus, which has overgrown the material better. The best fungal overgrowth of the substrate was for a mixture with a ratio of 60:40. The fungi grew also over mixtures which had no addition of straw (mixture 100: 0), but much worse than for mixtures with addition of straw. The amount of lignin was decreased with the time of overgrowth because the fungi decompose lignin, while the proportion of carbohydrates has increased. Using the GC-MS method, the proof that fungi would degrade lignocelluloses into monosaccharides was not successful or the presence of monosaccharides was below the detection limit for analysis with such method.|