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Title:Critical and supercritical spatiotemporal calcium dynamics in beta cells
Authors:ID Gosak, Marko (Author)
ID Stožer, Andraž (Author)
ID Markovič, Rene (Author)
ID Dolenšek, Jurij (Author)
ID Perc, Matjaž (Author)
ID Rupnik, Marjan (Author)
ID Marhl, Marko (Author)
Files:.pdf Frontiers_in_Physiology_2017_Gosak_et_al._Critical_and_Supercritical_Spatiotemporal_Calcium_Dynamics_in_Beta_Cells.pdf (3,43 MB)
MD5: 4EA161605A3548418E7DCD104D7CA2CA
PID: 20.500.12556/dkum/541d2df0-2f56-4270-89c3-ce0c5d6aad34
URL http://journal.frontiersin.org/article/10.3389/fphys.2017.01106/full
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Abstract:A coordinated functioning of beta cells within pancreatic islets is mediated by oscillatory membrane depolarization and subsequent changes in cytoplasmic calcium concentration. While gap junctions allow for intraislet information exchange, beta cells within islets form complex syncytia that are intrinsically nonlinear and highly heterogeneous. To study spatiotemporal calcium dynamics within these syncytia, we make use of computational modeling and confocal high-speed functional multicellular imaging. We show that model predictions are in good agreement with experimental data, especially if a high degree of heterogeneity in the intercellular coupling term is assumed. In particular, during the first few minutes after stimulation, the probability distribution of calcium wave sizes is characterized by a power law, thus indicating critical behavior. After this period, the dynamics changes qualitatively such that the number of global intercellular calcium events increases to the point where the behavior becomes supercritical. To better mimic normal in vivo conditions, we compare the described behavior during supraphysiological non-oscillatory stimulation with the behavior during exposure to a slightly lower and oscillatory glucose challenge. In the case of this protocol, we observe only critical behavior in both experiment and model. Our results indicate that the loss of oscillatory changes, along with the rise in plasma glucose observed in diabetes, could be associated with a switch to supercritical calcium dynamics and loss of beta cell functionality.
Keywords:beta cells, islets of Langerhans, self-organized criticality, intercellular dynamics, calcium waves, glucose oscillations, computational model, confocal calcium imaging
Publication status:Published
Publication version:Version of Record
Year of publishing:2017
Number of pages:str. 1-17
Numbering:Letn. 8
PID:20.500.12556/DKUM-69461 New window
ISSN on article:1664-042X
COBISS.SI-ID:512760376 New window
DOI:10.3389/fphys.2017.01106 New window
Publication date in DKUM:23.01.2018
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Record is a part of a journal

Title:Frontiers in physiology
Shortened title:Front. physiol.
Publisher:Frontiers Research Foundation
COBISS.SI-ID:1218939 New window


License:CC BY 4.0, Creative Commons Attribution 4.0 International
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:23.01.2018

Secondary language

Keywords:beta celice, Langerhansovi otočki, samoorganizirana kritičnost, medcelična dinamika, kalcijevi valovi, oscilacije glukoze, računski model


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