| Title: | Simultaneous GC-MS determination of free and bound phenolic acids in Slovenian red wines and chemometric characterization |
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| Authors: | ID Ivanović, Milena (Author)
ID Islamčević Razboršek, Maša (Author)
ID Kolar, Mitja (Author) |
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| Files: |  Acta_Chimica_Slovenica_2016_Ivanovic,_Islamcevic_Razborsek,_Kolar_Simultaneous_GC-MS_Determination_of_Free_and_Bound_Phenolic_Acids_in_S.pdf (222,27 KB)
MD5: 4737ED92FC39808498566BF744EAE062
PID: 20.500.12556/dkum/74c16350-14a1-4185-89e4-7fe0f9b35545
 https://journals.matheo.si/index.php/ACSi/article/view/2534
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
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| Abstract: | Several phenolic acids (PAs), caffeic, vanillic, syringic, p-coumaric and ferulic acid, found in Slovenian red wines were studied using gas chromatography and mass spectrometry (GC-MS). For isolation of the PAs from wine samples, solid phase extraction (SPE) using hydrophilic modified styrene - HLB cartridges was used. The bound PAs were extracted after basic hydrolysis and o-coumaric acid was used as the internal standard (ISTD). The method developed was validated and the linear concentration range for all analytes was from 1 to 100 mg L$^{-1}$ with correlation coefficients above 0.999. We show that the method is repeatable (RSD<2%), recoveries were above 96%, and LOD and LOQ values were acceptable.
In all of the wine samples tested, caffeic and p-coumaric acid were determined to be the predominant PAs (17-72 mg L$^{-1}$), while other compounds were found in lower concentrations. Principal Component Analysis (PCA) and Cluster Analysis (CLU) were used to study differences between wines related towards varieties and Slovenian wine regions. The results demonstrate that variety has more influence on PAs content than wine regions in Slovenian red wines. |
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| Keywords: | phenolic acids, Slovenian red wines, gas chromatography, mass spectrometry, PCA, CLU |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2016 |
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| Number of pages: | str. 661-669 |
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| Numbering: | Letn. 63, št. 3 |
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| PID: | 20.500.12556/DKUM-67640  |
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| ISSN: | 1318-0207 |
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| UDC: | 66 |
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| ISSN on article: | 1318-0207 |
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| COBISS.SI-ID: | 19728662 |
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| DOI: | 10.17344/acsi.2016.2534 |
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| NUK URN: | URN:SI:UM:DK:UMTW2EKZ |
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| Publication date in DKUM: | 25.08.2017 |
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| Views: | 2231 |
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| Downloads: | 388 |
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| Metadata: |    |
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| Categories: | Misc.
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