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Title:Simultaneous GC-MS determination of free and bound phenolic acids in Slovenian red wines and chemometric characterization
Authors:ID Ivanović, Milena (Author)
ID Islamčević Razboršek, Maša (Author)
ID Kolar, Mitja (Author)
Files:.pdf Acta_Chimica_Slovenica_2016_Ivanovic,_Islamcevic_Razborsek,_Kolar_Simultaneous_GC-MS_Determination_of_Free_and_Bound_Phenolic_Acids_in_S.pdf (222,27 KB)
MD5: 4737ED92FC39808498566BF744EAE062
PID: 20.500.12556/dkum/74c16350-14a1-4185-89e4-7fe0f9b35545
 
URL https://journals.matheo.si/index.php/ACSi/article/view/2534
 
Language:English
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Several phenolic acids (PAs), caffeic, vanillic, syringic, p-coumaric and ferulic acid, found in Slovenian red wines were studied using gas chromatography and mass spectrometry (GC-MS). For isolation of the PAs from wine samples, solid phase extraction (SPE) using hydrophilic modified styrene - HLB cartridges was used. The bound PAs were extracted after basic hydrolysis and o-coumaric acid was used as the internal standard (ISTD). The method developed was validated and the linear concentration range for all analytes was from 1 to 100 mg L$^{-1}$ with correlation coefficients above 0.999. We show that the method is repeatable (RSD<2%), recoveries were above 96%, and LOD and LOQ values were acceptable. In all of the wine samples tested, caffeic and p-coumaric acid were determined to be the predominant PAs (17-72 mg L$^{-1}$), while other compounds were found in lower concentrations. Principal Component Analysis (PCA) and Cluster Analysis (CLU) were used to study differences between wines related towards varieties and Slovenian wine regions. The results demonstrate that variety has more influence on PAs content than wine regions in Slovenian red wines.
Keywords:phenolic acids, Slovenian red wines, gas chromatography, mass spectrometry, PCA, CLU
Publication status:Published
Publication version:Version of Record
Year of publishing:2016
Number of pages:str. 661-669
Numbering:Letn. 63, št. 3
PID:20.500.12556/DKUM-67640 New window
ISSN:1318-0207
UDC:66
ISSN on article:1318-0207
COBISS.SI-ID:19728662 New window
DOI:10.17344/acsi.2016.2534 New window
NUK URN:URN:SI:UM:DK:UMTW2EKZ
Publication date in DKUM:25.08.2017
Views:1972
Downloads:372
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Categories:Misc.
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Record is a part of a journal

Title:Acta Chimica Slovenica
Shortened title:Acta Chim. Slov.
Publisher:Slovensko kemijsko društvo
ISSN:1318-0207
COBISS.SI-ID:14086149 New window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:25.08.2017

Secondary language

Language:Slovenian
Abstract:Izbrane fenolne kisline: kavno, vanilinsko, siringinsko, kumarinsko in ferulno smo v različnih rdečih slovenskih vinih analizirali z uporabo GC-MS. Za izolacijo smo uporabljali ekstrakcijski postopek na HLB SPE kolonicah, ki vsebuje hidrofilno modificiran stiren. Pri delu smo vezane fenolne kisline hidrolizirali, kot interni standard pa smo uporabljali orto-kumarinsko kislino. Razvito metodo smo validirali: linearno koncentracijsko območje fenolnih kislin je med 1 in 100 mg L$^{-1}$, korelacijski koeficienti so bili nad 0,999. Potrdili smo dobro ponovljivost, RSD je znašal pod 2%, izkoristke nad 96% in sprejemljive vrednosti LOD ter LOQ. Ugotovili smo, da sta v vzorcih slovenskih vin prevladujoči fenolni spojini kavna in para-kumarna kislina (17–72 mg L$^{-1}$), medtem ko ostale spojine najdemo v nižjem koncentracijskem območju. Metodo glavnih osi (PCA) in analizo klastrov (CLU) smo uporabili za študij podobnosti in razlik med vzorci glede vsebnosti in deležev fenolnih kislin. Potrdili smo korelacijo in večji vpliv sorte grozdja kot vinorodne dežele oziroma regije, iz katere vzorci vin izvirajo.
Keywords:fenolni acidi, slovenska rdeča vina, plinska kromatografija, masna spektrometrija


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