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Title:Študija učinkovitosti imobilizacije beta galaktozidaze na zvitke steklene tkanine
Authors:ID Zečević, Katja (Author)
ID Pečar, Darja (Mentor) More about this mentor... New window
ID Goršek, Andreja (Comentor)
Files:.pdf VS_Zecevic_Katja_2017.pdf (1,87 MB)
MD5: 6AA23120CA8F39F5123348AF14D63F6E
PID: 20.500.12556/dkum/29341ead-2226-4b38-9847-cea93cb991e5
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:V tej raziskavi smo razvili novo metodo imobilizacije β-galaktozidaze na zvitke steklene tkanine. Raziskali smo uporabnost steklene tkanine kot vlaknastega nosilca z visoko poroznostjo in odprto strukturo. Nov postopek zagotavlja preprosto, cenovno ugodno in industrijsko primerno metodo imobilizacije. Glutaraldehid smo uporabili kot nespecifično povezovalno sredstvo za imobilizacijo β-galaktozidaze na stekleno tkanino. Le-ta je omogočal kovalentno vezavo encima na modificiran nosilec. Učinkovitost imobilizacije encima smo testirali na dobro poznani reakciji hidrolize laktoze. Vse eksperimente smo izvajali v laboratorijskem kontinuirnem reaktorju. Preučevali smo vpliv temperature,  = (20, 25 in 30) °C, pretoka, qV = (1, 2 in 3) mL/min in pH = (6, 7 in 8) na stabilnost in aktivnost β-galaktozidaze med hidrolizo. Presnovo laktoze smo določali z merjenjem koncentracije glukoze. Dosegli smo visoko učinkovitost imobilizacije in končno aktivnost encima. Z večkratno zaporedno uporabo istega imobiliziranega encima je ostala presnova praktično nespremenjena, kar kaže na možno uporabo pri industrijskih aplikacijah.
Keywords:β-galaktozidaza, steklena tkanina, laktoza, imobilizacija
Place of publishing:Maribor
Publisher:[K. Zečević]
Year of publishing:2017
PID:20.500.12556/DKUM-67573 New window
UDC:637.345:544.478.3(043.2)
COBISS.SI-ID:21042966 New window
NUK URN:URN:SI:UM:DK:9J1CFT5P
Publication date in DKUM:14.09.2017
Views:1371
Downloads:263
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:23.08.2017

Secondary language

Language:English
Title:Immobilization of beta galactosidase on glass cloth rolls - efficiency study
Abstract:In this study we developed a method for β-galactosidase immobilization using special glass cloth rolls as a new designed support. The effectiveness of glass cloth as a fibrous matrix with high porosity and open structure was explored. The new procedure provides simple, inexpensive and industrially applicable method and also support for active and stable immobilized biocatalyst. Glutaraldehyde was used as a non-specific cross-linking agent for fixing the β-galactosidase on the glass cloth. Enzyme was covalently bonded on modified support. We tasted the efficiency- of bonding on well-known hydrolysis of lactose. All experiments were carried out in the laboratory continuous reactor. The influence of reaction temperature (20, 25 and 30 °C), substrate flow rate (1, 2 and 3 mL/min) and pH (6, 7, 8) on reaction conversion were studied. Reaction efficiency was monitored by measuring the glucose concentration using spectrophotometer. We reached high immobilization efficiency and final enzyme activity. The optimum reaction temperature, substrate flow rate and pH were found. β-galactosidase immobilized on glass cloth rolls was successfully used in continuous biocatalyst reactor for hydrolysis of lactose. Activity and stability during the reuse of enzyme entrapped on the glass cloth roll remained almost unchanged, showing high perspective for possible industrial applications.
Keywords:β-galactosidase, glass cloth, lactose, immobilization


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